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#DAGD-100 QuantiChrom™ á_Glucosidase Assay Kit
Product name : QuantiChrom™ á_Glucosidase Assay Kit
Catalog number : DAGD-100
Supplier name : Bioassays
Data sheet: Ask more or other datasheet now !
About this Product :QuantiChrom™ á_Glucosidase Assay Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ á_Glucosidase Assay Kit.
More Details about
For quantitative determination of α-glucosidase activity and evaluation of drug effects on its metabolism.
Procedure: 20 min.
Size: 100 tests.
Detection limit: 2 U/L.
α-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked α-Dglucose
residues with release of α-D-glucose. α-Glucosidase is needed by
all animals to hydrolyze maltose to glucose for use as a food. Aberrant
activities have been implicated in diseases such as diabetes and Pompe
Simple, direct and automation-ready procedures for measuring α-
glucosidase activity are becoming popular in Research and Drug Discovery.
BioAssay Systems' QuantiChromTM α-Glucosidase Assay Kit is designed to
measure α-glucosidase activity directly in biological samples without
pretreatment. The improved method utilizes p-nitrophenyl-α-Dglucopyranoside
that is hydrolyzed specifically by α-glucosidase into a
yellow colored product (maximal absorbance at 405nm). The rate of the
reaction is directly proportional to the enzyme activity.
High sensitivity and wide linear range. Use 20 μL sample. The detection
limit is 2 U/L, linear up to 250 U/L.
Homogeneous and simple procedure. Simple “mix-and-measure”
procedure allows reliable quantitation of α-glucosidase activity within 20
Robust and amenable to HTS. All reagents are compatible with highthroughput
liquid handling instruments.
Direct Assays: α-glucosidase activity in biological samples.
Characterization and Quality Control for α-glucosidase production.
Drug Discovery: high-throughput screen and evaluation of α-glucosidase
KIT CONTENTS (100 tests in 96-well plates)
Assay Buffer: 24 mL (pH 7.0)
a-NPG Substrate: 1 mL
Calibrator: 10 mL (equivalent to 250 U/L)
Storage conditions. The kit is shipped at room temperature. Store all
components at -20°C. Shelf life of at least 6 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
MATERIALS REQUIRED, BUT NOT PROVIDED
Pipeting devices and accessories (e.g. multi-channel pipettor). Clear
bottom 96-well plates (e.g. Corning Costar) and plate reader.
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