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#DICU-250 QuantiChrom™ Copper Assay Kit
Product name : QuantiChrom™ Copper Assay Kit
Catalog number : DICU-250
Supplier name : Bioassays
Data sheet: Ask more or other datasheet now !
About this Product :QuantiChrom™ Copper Assay Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ Copper Assay Kit.
More Details about
Copper is an essential trace element. Copper-containing enzymes play
important roles in iron and catecholamine metabolism, free radical
scavenging, and in the synthesis of hemoglobin, elastin and collagen. Copper
is mainly present in caeruloplasmin in the liver. Low levels of copper have
been associated with mental retardation, depigmentation, anaemia,
hypotonia and scorbutic changes in bone. Levels of copper are key
diagnostic indicator of diseases such as Wilson's disease, microcytic
hypochromic anaemia and bone disease due to reduced collagen synthesis.
Simple, direct and automation-ready procedures for measuring copper
concentrations find wide applications in research, drug discovery and
environmental monitoring. BioAssay Systems' copper assay kit is
designed to measure copper with no or minimal sample treatment. The
improved method utilizes a chromogen that forms a colored complex
specifically with copper ions. The intensity of the color, measured at
359nm, is directly proportional to copper concentration in the sample.
The optimized formulation substantially reduces interference by
substances in the raw samples.
Sensitive and accurate. Linear detection range 7 μg/dL (1.0 μM) to 300
μg/dL (47 μM) copper in 96-well plate assay.
Simple and high-throughput. The simple procedure can be readily
automated as a high-throughput assay in 96-well plates for thousands of
samples per day.
Improved reagent stability and versatility. The optimized formulation has
greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
Direct Assays: biological, environmental, food and beverage samples.
Drug Discovery/Pharmacology: effects of drugs on Cu metabolism.
KIT CONTENTS (250 tests in 96-well plates)
Reagent A: 10 mL Reagent B: 1.5 mL Reagent C: 40 mL
Copper Standard: 1 mL 1.5 mg/dL Cu2+
Storage conditions. The kit is shipped at room temperature. Store all
reagents at 4 °C. Shelf life: 12 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
Please refer to Material Safety Data Sheet for detailed information.
Note: metal chelators (e.g. EDTA) interfere with this assay and should be
avoided in sample preparation.
Procedure using 96-well plate:
1. Standards: transfer 100 μL dH2O into one Eppendorf tube labeled
“Blank”. Into another tube labeled “Standard”, mix 20 μL 1.5 mg/dL
Standard and 80 μL dH2O (final 300 μg/dL Cu2+).
Samples: transfer 100 μL samples into separate tubes.
Add 35 μL Reagent A (trichloroacetic acid) to each tube and mix by
vortexing. If samples contain protein (e.g. serum/plasma), precipitates
form. Centrifuge tubes for 2 min at 14,000 rpm and use clear
supernatant for assay. For samples that do not contain protein, the
mixture remains clear and centrifugation is not necessary.
Transfer 100 μL Blank, Standard and Sample into separate wells of a
clear flat-bottom 96-well plate.
2. For each assay well, prepare Working Reagent by mixing 5 μL Reagent
B and 150 μL Reagent C. Transfer 150 μL Working Reagent to each
well and tap plate to mix thoroughly.
3. Incubate 5 min at room temperature and read optical density at 356-
362nm (peak absorbance at 359nm).
Note: if sample OD values are higher than the OD value for the 300μg/dL
Standard, dilute sample in dH2O and repeat assay. Multiply the results by the
Procedure using cuvette:
Prepare standards and samples as for 96-well assay procedure.
1. Transfer 400 μL Standards and Samples into separate cuvets.
2. Add 600 μL Working Reagent. Mix by pipetting.
3. Incubate 5 min at room temperature and read optical density at
356-362nm (peak absorbance at 359nm).
The copper concentration of Sample is calculated as
ODSAMPLE – ODBLANK
ODSTANDARD – ODBLANK
× 300 (μg/dL)
ODSAMPLE, ODBLANK and ODSTANDARD are optical density values of the
Sample, Blank and the 300 μg/dL Standard, respectively.
Conversions: 100 μg/dL Cu equals 15.5 μM, 0.0001% or 1 ppm.
MATERIALS REQUIRED, BUT NOT PROVIDED
Pipeting devices and accessories. For 96-well plate assays: clear
flat-bottom 96-well plates and plate reader. For cuvet assays:
spectrophotometer and cuvets for measuring OD at 356-362nm.
For scarce samples (e.g. mice serum or plasma), mix sample with
dH2O to a total of 100 μL, e.g. 50 μL serum + 50 μL dH2O. Multiply
the results by the dilution factor (2 fold).
Human serum, rat plasma, rat serum, and bovine serum were
assayed in duplicate using the 96-well plate assay protocol. The
copper concentrations were 97 ± 1, 104 ± 1, 101 ± 2, 78 ± 1 μg/dL,
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