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#DIHM-250 QuantiChrom™ Heme Assay Kit
Product name : QuantiChrom™ Heme Assay Kit
Catalog number : DIHM-250
Supplier name : Bioassays
Data sheet: Ask more or other datasheet now !
About this Product :QuantiChrom™ Heme Assay Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ Heme Assay Kit.
More Details about
Heme is one important member of the porphyrin family. It is synthesized
in both mitochondria and cytoplasm, and is a key prosthetic group for
various essential proteins such as hemoglobin, cytochromes, catalases
and peroxidases. Heme determination is widely practiced by researchers
of various blood diseases.
Simple, direct and automation-ready procedures for measuring heme
concentration are becoming popular in Research and Drug Discovery.
BioAssay Systems' QuantiChromTM Heme Assay Kit is based on an
improved aqueous alkaline solution method, in which the heme is
converted into a uniform colored form. The intensity of color, measured
at 400 nm, is directly proportional to the heme concentration in the
sample. The optimized formulation substantially reduces interference by
substances in the raw samples and exhibits high sensitivity.
Direct Assays: total heme in blood, serum, plasma, urine, hemecarrying
Pharmacology: effects of drugs on heme metabolism.
Drug Discovery: HTS for drugs that modulate heme levels.
Sensitive and accurate. Linear detection range 0.6 – 125 μM heme in
96-well plate assay.
Simple and high-throughput. The “mix-and-read” procedure involves
addition of a single working reagent and reading the optical density. Can
be readily automated as a high-throughput assay in 96-well plates for
thousands of samples per day.
Safety. Reagents are non-toxic.
Versatility. Assays can be executed in 96-well plate or cuvet.
KIT CONTENTS (250 tests in 96-well plates)
Reagent: 50 mL
Calibrator: 10 mL (equivalent to 62.5 μM heme)
Storage conditions. The kit is shipped room temperature. Store reagent
and Calibrator at 4°C. Shelf life: 12 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
Please refer to Material Safety Data Sheet for detailed information.
MATERIALS REQUIRED, BUT NOT PROVIDED
Pipeting devices and accessories.
Procedure using 96-well plate:
Clear-bottom 96-well plates (e.g. Corning Costar) and plate reader.
Procedure using cuvette:
Cuvets and spectrophotometer.
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WP1004: Kit Receptor Signaling Pathway
WP102: Heme Biosynthesis
WP1086: Heme Biosynthesis
WP1121: Kit Receptor Signaling Pathway
WP1221: Heme Biosynthesis
WP1314: Heme Biosynthesis
WP1341: Kit Receptor Signaling Pathway
WP147: Kit Receptor Signaling Pathway
WP1689: Porphyrin and chlorophyll metabolism
WP18: Heme Biosynthesis
WP269: Heme Biosynthesis
WP407: Kit Receptor Signaling Pathway
WP548: neural crest development
WP561: Heme Biosynthesis
WP774: Kit Receptor Signaling Pathway
WP848: Heme Biosynthesis
WP86: Heme Biosynthesis
WP886: Kit Receptor Signaling Pathway
WP967: Heme Biosynthesis
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