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#DIOX-250 QuantiChrom™ Peroxide Assay Kit
Product name : QuantiChrom™ Peroxide Assay Kit
Catalog number : DIOX-250
Supplier name : Bioassays
Data sheet: Ask more or other datasheet now !
About this Product :QuantiChrom™ Peroxide Assay Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ Peroxide Assay Kit.
More Details about
Peroxide (e.g. hydrogen peroxide H2O2) is one of the key reactive
oxygen species formed under oxidative stress conditions. High levels of
peroxide formation have been linked to pathological conditions such as
ageing, asthma, diabetes, atherosclerosis, cataract, inflammatory
arthritis and neurodegenerative diseases.
Simple, direct and automation-ready procedures for quantitative
determination of peroxide find wide applications in research and drug
discovery. BioAssay Systems' peroxide assay kit is designed to measure
peroxide concentration in biological samples without any pretreatment.
The improved method utilizes the chromogenic Fe3+-xylenol orange
reaction, in which a purple complex is formed when Fe2+ provided in the
reagent is oxidized to Fe3+ by peroxides present in the sample. The
intensity of the color, measured at 540-610nm, is an accurate measure
of the peroxide level in the sample. The optimized formulation
substantially reduces interference by substances in the raw samples.
Sensitive and accurate. Enhanced color intensity using sorbitol.
Detection range 0.2 μM (7 ng/mL) to 30 μM (1,020 ng/mL) H2O2 in 96-well
Simple and high-throughput. The procedure involves addition of a
single detection reagent and incubation for 30 min. Can be readily
automated as a high-throughput assay in 96-well plates for thousands of
samples per day.
Direct Assays: H2O2 in biological samples (e.g. serum, citrate-plasma,
urine, cell lysate, culture medium).
Pharmacology: effects of drugs on peroxide metabolism.
KIT CONTENTS (250 tests in 96-well plates)
Reagent A: 1 mL
Reagent B: 50 mL
Standard: 100 μL 3% stabilized H2O2.
Kit shipped at room temperature.
Storage conditions. The kit is shipped at room temperature. Store all
reagents at 4 °C. Shelf life: 6 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
Please refer to Material Safety Data Sheet for detailed information.
MATERIALS REQUIRED, BUT NOT PROVIDED
Pipetting devices and accessories, 96-well plates and plate reader.
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Support Karolina Elandt
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Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
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