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#ECLC-100 EnzyChrom™ Lactate Assay Kit
Product name : EnzyChrom™ Lactate Assay Kit
Catalog number : ECLC-100
Supplier name : Bioassays
Data sheet: Ask more or other datasheet now !
More Details aboutThe EnzyChrom™ L-Lactate Assay Kit is made for quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. The EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.#2#http://biotech-map.com/aa_search/table_sample.html?q=EnzyChrom%E2%84%A2%20L-Lactate%20Assay%20Kit&filter=
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