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#EDLC-100 EnzyChrom™ D_Lactate Assay Kit
Product name : EnzyChrom™ D_Lactate Assay Kit
Catalog number : EDLC-100
Supplier name : Bioassays
Data sheet: Ask more or other datasheet now !
More Details aboutThe EnzyChrom™ D-Lactate Assay Kit is made for quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. The EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.#2#http://biotech-map.com/aa_search/table_sample.html?q=EnzyLight%E2%84%A2%20Cytotoxicity%20Assay%20Kit&filter=
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