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Index / Bioassays / pNPP Phosphatase Assay Kit / Product Detail : POPN-01K pNPP Phosphatase Assay Kit
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Jul
29th

#POPN-01K pNPP Phosphatase Assay Kit

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  Price : 360   EUR
409   USD
279   GBP
1514   Zloty
48238   JPY
2780   NOK
2979   SEK
407   CHF

Product name : pNPP Phosphatase Assay Kit

Catalog number : POPN-01K

Quantity: 1000

Availability: Yes

Supplier name : Bioassays

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More Details about

For quantitative determination of phosphatase activity.
Method: OD405nm.
Samples: protein phosphatases.
Species: all.
Procedure: 30 min.
Size: 1000 tests.
Detection limit: 3 ng.

DESCRIPTION
Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most
phosphatases such as alkaline phosphatases, acid phosphatases, protein
tyrosine phosphatases and serine/threonine phosphatases. The reaction
yields para-nitrophenol, which becomes an intense yellow soluble product
under alkaline conditions and can be conveniently measured at 405 nm on
a spectrophotometer.
phosphatase
p-Nitrophenyl phosphate p-nitrophenol + phosphate
This homogeneous "mix-and-measure" assay involves simply adding a
single reagent to the phosphatase and measuring the product formation
on an absorbance reader. The assay can be conveniently performed in
cuvettes, tubes or multi-well plates at either room temperature or 37°C.
KEY FEATURES
High sensitivity and wide linear range. The detection limit is generally 3
ng phosphatase or below.
Homogeneous and simple procedure. No wash or reagent transfer
steps are involved. The assay can be completed within 30 minutes.
Robust and amenable to HTS. All reagents are compatible with highthroughput
liquid handling instruments.
APPLICATIONS
Enzyme Activity Assay and Quality Control for phosphatase
production.
Characterization of Kinetics of phosphatase reaction.
Drug Discovery: high-throughput screen for phosphatase inhibitors.
KIT CONTENTS
Catalog # Size (assays) Reagent Assay Buffer Stop Solution
POPN-500 500 280 μL 25 mL 25 mL
POPN-01K 1,000 550 μL 50 mL 50 mL
POPN-HTS >10k customized customized customized
Storage conditions. The kit is shipped at ambient temperature. Store
Reagent at -20°C and other components at 4°C. Shelf life: 12 months after
receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents. Please
refer to Material Safety Data Sheet for detailed information.

 

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