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Index / BPS Bioscience / Fast, Simple & Efficient Cloning Kit without competent cell / Product Detail : 20100 Fast, Simple & Efficient Cloning Kit without competent cell
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#20100 Fast, Simple & Efficient Cloning Kit without competent cell

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  Price : 1170   EUR
1328   USD
908   GBP
4916   Zloty
156595   JPY
9025   NOK
9670   SEK
1323   CHF

Product name : Fast, Simple & Efficient Cloning Kit without competent cell

Catalog number : 20100

Quantity: 100 reactions

Availability: Yes

Supplier name : BPS Bioscience

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More Details about

Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. The activation of caspase 3/7 is important for the initiation of apoptosis. It has been proven that caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses FAM-DEVD-FMK as a fluorescent indicator for caspase 3/7 activity. FAM-DEVD-FMK irreversibly binds to activated caspase 3/7 in apoptotic cells. Once bound to caspase 3/7, the fluorescent reagent is retained inside the cell. The binding event inhibits caspase 3/7 but will not stop apoptosis from proceeding. There are a variety of parameters that can be used for monitoring cell apoptosis. This Cell Meter™ Live Cell Caspase 3/7 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 3/7 activation in live cells. It is used for the quantification of activated caspase 3/7 activities in apoptotic cells, or for screening caspase 3/7 inhibitors. FAM-DEVD-FMK, the green label reagent, allows for direct detection of activated caspase 3/7 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.

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