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#1216-100 TMB, High Kinetics
Product name : TMB, High Kinetics
Catalog number : 1216-100
Quantity: 100 ml
Supplier name : Biovis
Data sheet: Ask more or other datasheet now !
More Details about
Alternate Name/Synonyms: 3,3',5,5'-Tetramethylbenzidine; TMBHK
Peptide sequence: N/A
Formulation: 2.5 mMol L⁻¹ TMB and Hydrogen Peroxide in a proprietary buffer at pH 3.6.
CAS Number: 54827-17-7
Molecular Formula: C₁₆H₂₀N₂
Molecular Weight: 240.35
Storage Temp.: +4°C
Shipping Conditions: RT
Description: 3,3',5,5'-Tetramethylbenzidine (TMB) has been shown to be a safe, sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum at 653 nm. Further reaction with HRP/H₂O₂ or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 3.9 x 10⁴ mol⁻¹ cm⁻¹) and diimine (E450 nm = 5.9 x 10⁴ mol⁻¹ cm⁻¹ ) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMBHK, available from BioVision Inc., is a highly sensitive single component reagent that is ready to use for the quantitative detection of HRP bound to a solid phase or in free solution. TMBHK is stable at room temperature for six months and is not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP commonly employed in immunologic assays.
Handling: Protect from air and moisture
USAGE: For Research Use Only! Not For Use in Humans.
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[mutM fpg Thimo_3629] Formamidopyrimidine-DNA glycosylase (Fapy-DNA glycosylase) (EC 220.127.116.11) (DNA-(apurinic or apyrimidinic site) lyase MutM) (AP lyase MutM) (EC 18.104.22.168)
[nth Thimo_1808] Endonuclease III (EC 22.214.171.124) (DNA-(apurinic or apyrimidinic site) lyase)
[lpxC Thimo_3104] UDP-3-O-acyl-N-acetylglucosamine deacetylase (UDP-3-O-acyl-GlcNAc deacetylase) (EC 126.96.36.199) (UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase)
[plsY Thimo_0558] Glycerol-3-phosphate acyltransferase (Acyl-PO4 G3P acyltransferase) (Acyl-phosphate--glycerol-3-phosphate acyltransferase) (G3P acyltransferase) (GPAT) (EC 2.3.1.n3) (Lysophosphatidic acid synthase) (LPA synthase)
[plsX Thimo_2383] Phosphate acyltransferase (EC 2.3.1.n2) (Acyl-ACP phosphotransacylase) (Acyl-[acyl-carrier-protein]--phosphate acyltransferase) (Phosphate-acyl-ACP acyltransferase)
[nnrE nnrD Thimo_1694] ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC 188.8.131.52) (EC 184.108.40.206) (ADP-dependent NAD(P)HX dehydratase) (NAD(P)H-hydrate epimerase) (NAD(P)HX epimerase)
[cca Thimo_3398] Multifunctional CCA protein [Includes: Phosphatase (EC 3.1.3.-); 2',3'-cyclic phosphodiesterase (EC 3.1.4.-); 2'-nucleotidase; CCA-adding enzyme (EC 220.127.116.11) (CCA tRNA nucleotidyltransferase) (tRNA CCA-pyrophosphorylase) (tRNA adenylyl-/cytidylyl-transferase) (tRNA nucleotidyltransferase) (tRNA-NT)]
[pyrG Thimo_1321] CTP synthase (EC 18.104.22.168) (Cytidine 5'-triphosphate synthase) (Cytidine triphosphate synthetase) (CTP synthetase) (CTPS) (UTP--ammonia ligase)
[leuB Thimo_2654] 3-isopropylmalate dehydrogenase (EC 22.214.171.124) (3-IPM-DH) (Beta-IPM dehydrogenase) (IMDH)
[Thimo_0751] Non-canonical purine NTP pyrophosphatase (EC 126.96.36.199) (Non-standard purine NTP pyrophosphatase) (Nucleoside-triphosphate diphosphatase) (Nucleoside-triphosphate pyrophosphatase) (NTPase) (Nucleotide diphosphatase)
[dapB Thimo_2534] 4-hydroxy-tetrahydrodipicolinate reductase (HTPA reductase) (EC 188.8.131.52)
[ribB Thimo_0212] 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase) (EC 184.108.40.206)
[coaX Thimo_1347] Type III pantothenate kinase (EC 220.127.116.11) (PanK-III) (Pantothenic acid kinase)
[Thimo_0502] Bifunctional adenosylcobalamin biosynthesis protein (EC 18.104.22.168) (EC 22.214.171.124)
[dusA Thimo_2204] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 126.96.36.199) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[katG Thimo_3545] Catalase-peroxidase (CP) (EC 188.8.131.52) (Peroxidase/catalase)
[dapE Thimo_2017] Succinyl-diaminopimelate desuccinylase (SDAP desuccinylase) (EC 184.108.40.206) (N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase)
[pdxH Thimo_2905] Pyridoxine/pyridoxamine 5'-phosphate oxidase (EC 220.127.116.11) (PNP/PMP oxidase) (PNPOx) (Pyridoxal 5'-phosphate synthase)
[recB Thimo_2316] RecBCD enzyme subunit RecB (EC 18.104.22.168) (Exonuclease V subunit RecB) (ExoV subunit RecB) (Helicase/nuclease RecBCD subunit RecB)
[mobA Thimo_0419] Molybdenum cofactor guanylyltransferase (MoCo guanylyltransferase) (EC 22.214.171.124) (GTP:molybdopterin guanylyltransferase) (Mo-MPT guanylyltransferase) (Molybdopterin guanylyltransferase) (Molybdopterin-guanine dinucleotide synthase) (MGD synthase)
[birA Thimo_0106] Bifunctional ligase/repressor BirA (Biotin operon repressor) (Biotin--[acetyl-CoA-carboxylase] ligase) (EC 126.96.36.199) (Biotin--protein ligase) (Biotin-[acetyl-CoA carboxylase] synthetase)
[lon Thimo_2889] Lon protease (EC 188.8.131.52) (ATP-dependent protease La)
[sucC Thimo_0241] Succinate--CoA ligase [ADP-forming] subunit beta (EC 184.108.40.206) (Succinyl-CoA synthetase subunit beta) (SCS-beta)
[ileS Thimo_1139] Isoleucine--tRNA ligase (EC 220.127.116.11) (Isoleucyl-tRNA synthetase) (IleRS)
[carB Thimo_0297] Carbamoyl-phosphate synthase large chain (EC 18.104.22.168) (Carbamoyl-phosphate synthetase ammonia chain)
[carA Thimo_0298] Carbamoyl-phosphate synthase small chain (EC 22.214.171.124) (Carbamoyl-phosphate synthetase glutamine chain)
[pepA Thimo_2320] Probable cytosol aminopeptidase (EC 126.96.36.199) (Leucine aminopeptidase) (LAP) (EC 188.8.131.52) (Leucyl aminopeptidase)
[lon Thimo_0432] Lon protease (EC 184.108.40.206) (ATP-dependent protease La)
[dinB Thimo_2363] DNA polymerase IV (Pol IV) (EC 220.127.116.11)
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