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Index / SBI / miRZip_21 anti_miR_21 microRNA construct / Product Detail : MZIP21-PA-1 miRZip_21 anti_miR_21 microRNA construct
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Dec
8th

#MZIP21-PA-1 miRZip_21 anti_miR_21 microRNA construct

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  Price : 676   EUR
767   USD
524   GBP
2840   Zloty
90464   JPY
5214   NOK
5586   SEK
764   CHF

Product name : miRZip_21 anti_miR_21 microRNA construct

Catalog number : MZIP21-PA-1

Quantity: Bacterial streak

Availability: Yes

Supplier name : SBI

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About this Product :

miRZip_21 anti_miR_21 microRNA construct antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of miRZip_21 anti_miR_21 microRNA construct.
http://mybiofast.com/ver.php?search=anti-&submit=Search

miRZip_21 anti_miR_21 microRNA construct rna research rna is not so stable and very sticky. Rnases are abundand on hans and everywhere. SoreRNA at -75C and use rnase free.
http://gentaur-search.com/save_search/rna.htm

More Details about

Suppression of cell invasion and tumor metastasis by miRZip-21 and increased metastasis using miRZip-145

To investigate the roles of miR-21 (oncogenic microRNA) and miR-145 (tumor suppressor microRNA) in breast cancer, cell invasion assays of MDA-MB-231 breast cancer cells were performed after lentiviral transduction with control vector lentivirus (Control) or miRZip-21 or miRZip-145 lentivirus. Matrigel chamber assays were then used to screen for invasive phenotypes.

After 20 h, invasion cells attached to the lower surface were fixed, followed by staining with 0.05% crystal violet. The number of invaded cells on the membrane was then counted under a microscope. Representative fields of invasive cells on the membrane are shown at the bottom for the Control and miRZip-21 or miRZip-145 treated cells.

The miRZip-21 lentivirus expressing anti-mir-21 small RNAs inhibited metastasis by 80% and the miRZip-145 lentivirus expressing anti-miR-145 RNAs increased metastasis by 60%. Additionally, the miRZip-145 lentivirus inhibited endogenous miR-145 and elevated protein expression levels of the miR-145 target oncogene c-Myc.

• List of miRZip clones

 

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