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Index / Gene Link / Random Hexamers / Product Detail : 26-4000-03 Random Hexamers
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Aug
31th

#26-4000-03 Random Hexamers

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  Price : 131   EUR
149   USD
102   GBP
552   Zloty
17606   JPY
1014   NOK
1087   SEK
148   CHF

Product name : Random Hexamers

Catalog number : 26-4000-03

Quantity: 100ug

Availability: Yes

Supplier name : Gene Link

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More Details about

Random Hexamers; 100 ug
 
Random Primers are a mixture of oligonucleotides representing all possible sequence for that size.
 
Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5’ end cDNA sequence. 
 
Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. 
 
The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA. 
 
References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5.

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