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Index / Signosis 2011 / Cancer miRNA Profiling Plate Assay I / Product Detail : MA--0103 Cancer miRNA Profiling Plate Assay I
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#MA--0103 Cancer miRNA Profiling Plate Assay I

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  Price : 559   EUR
635   USD
434   GBP
2350   Zloty
74862   JPY
4314   NOK
4623   SEK
632   CHF

Product name : Cancer miRNA Profiling Plate Assay I

Catalog number : MA--0103

Quantity: 12

Availability: Yes

Supplier name : Signosis 2011

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About this Product :

Cancer miRNA Profiling Plate Assay I rna research rna is not so stable and very sticky. Rnases are abundand on hans and everywhere. SoreRNA at -75C and use rnase free.
http://gentaur-search.com/save_search/rna.htm

Cancer miRNA Profiling Plate Assay I exosomes mirnas are available in and out of exosomes. However exosomes are the perfect and easier way to obtain most pure exosomes except the argonauts
http://openwetware.org/wiki/Brown_BIOL1220:Notebook/SynBio_in_Theory_and_Practice/Genes/Argonauts(miRNA_pathway)

More Details about

 


miRNA Plate Assay Kit

MicroRNAs (miRNAs) are small noncoding RNAs that control gene expression at the posttranscriptional level through selectively binding to complementary messenger RNA sequences.  Approximately 30% of mammalian genes are regulated by these small RNA molecules, which affect a variety of biological functions, including development, cell differentiation, proliferation, apoptosis, and maintenance of stemness and imprinting. 
Northern blotting has been the most commonly used method for analyzing individual miRNAs, although recently a number of new approaches have been developed, such as the related real-time PCR and invader assays. Low sensitivity and poor throughput are the main issues of Northern blot analysis. To resolve this problem,  Signosis has developed the miRNA plate assay kits. The kits are highly sensitive, and the biochemical conversion of miRNA molecules into cDNAs is not needed. The procedure of the assay is as simple as incubation and wash.

To analyze a  miRNA with the plate assay, you need to purchase the common component miRNA plate assay kit (MA-0101) and  a specific oligo mix (MO-XXXX) corresponding to the miRNA.

Oligos avaible for miRNA Plate Assay Kit:

let-7a miR-22 miR-99a miR-135b miR-181a miR-198 miR-223 miR-720
let-7b miR-23a miR-100 miR-136 miR-181b miR-199a miR-224 miR-760
let-7c miR-24 miR-101-1 miR-137 miR-181c miR-199a* miR-296  
let-7d miR-25 miR-103 miR-140 miR-181d miR-199b miR-329  
let-7e miR-26a miR-106a miR-141 miR-182 miR-200a miR-337-3p  
let-7f miR-27a miR-106b miR-142-3p miR-183 miR-200c miR-338  
let-7g miR-28 miR-107 miR-142-5p miR-184 miR-202 miR-342  
let-7i miR-29a miR-122a miR-143 miR-185 miR-203 miR-345  
miR-1 miR-29b miR-124a miR-144 miR-186 miR-204 miR-368  
miR-7 miR-29c miR-125a miR-145 miR-188 miR-205 miR-369-3p  
miR-9 miR-30a-3p miR-125b miR-146a miR-190 miR-206 miR-369-5p  
miR-10a miR-30a-5p miR-126 miR-146b miR-191 miR-210 miR-371  
miR-15a miR-30b miR-127 miR-148a miR-192 miR-213 (miR-181a) miR-372  
miR-16 miR-30c miR-128a miR-149 miR-193a-3p miR-214 miR-373  
miR-17-3p miR-31 miR-128b miR-150 miR-193a-5p miR-215 miR-375  
miR-17-5p miR-92 miR-131 miR-151 miR-194 miR-216 miR-488  
miR-18a miR-92b miR-132 miR-153 miR-195 miR-218 miR-497  
miR-19a miR-93 miR-133a miR-152 miR-196a miR-219 miR-499-5p  
miR-20a miR-95 miR-133b miR-154 miR-196b miR-221 miR-507  
miR-21 miR-96 miR-134 miR-155 miR-197 miR-222 miR-609  

 




miR-21 Plate Assay Kit

miR-21 is overexpressed in a wide variety of cancers. It is believed to be an oncogene by downregulating the expression of many tumor suppressors, including programmed cell death 4 (PDCD4), tumor suppressor Pdcd4, the PTEN tumor suppressor gene, and the tumor suppressor gene tropomyosin 1 (TPM1). miR-21 is also found to be an important regulator of cancer-cell survival. Different from the survival pathway mediated by IL-6/Stat3 activation through the expression of antiapoptotic proteins, miR-21 is upregulated by Stat3, which mediates the suppression of apoptosis possibly through the inhibition of TPM1 and/or other proteins. Signosis’ miR-21 plate assay kit provides a simple and quick method to analyze this miRNA molecule.




miR-17-92 Cluster Plate Assay Kit

A polycistronic miRNA cluster miR-17-92 plays a role in the control of cell proliferation and angiogenesis. This cluster consists of seven miRNAs: miR-17-5p, miR-17-3p, miR-18a, miR-19a, miR-19b, miR-20a, and miR-92. Like E2F1 gene, the cluster is transcriptionally activated by the proto-oncogene c-Myc. Because two of the expressed miRNAs, miR-17-5p and miR-20a, repress the translation of E2F1, the level of E2F1 protein is modulated by this feedback mechanism.  Therefore, the accumulation of excessive amounts of E2F1 can be prevented and the states of cells towards apoptosis or cell division can be controlled. In addition, c-MYC-mediated induction of the miR-17-92 cluster results in down-regulation of the anti-angiogenic thrombospondin-1 and related proteins, such as connective tissue growth factor 2; Therefore, it promotes angiogenesis.  Furthermore, a recent study indicates that the cluster is significantly up-regulated at the clonal expansion stage of adipocyte differentiation. The miR-17-92 cluster has been implicated as the oncogenes in numerous tumor types.  Effective monitoring of the expression of the clustered miRNAs can help us better understand how the alteration of the cluster contributes to development of cancers and shed light on the molecular mechanisms of miRNA function.




Stem Cell-Specific miRNA Plate Assay Kit

Some of the miRNAs appear to be stem cell specific, and microarray analysis of miRNA expression can profile a set of unique miRNAs in stem cells. Signosis developed a panel of miRNA plate assay kits to analyze these miRNA molecules. Each kit can be used to profile 8 stem cell related miRNAs.

List of Applicable miRNAs:

Product name  miRNAS
Stem Cell-Specific miRNA Plate Assay Kit I Let7, miR-1, miR-9, miR-21, miR-24, miR-26a, miR-26b, RNU 48
Stem Cell-Specific miRNA Plate Assay Kit II miR-124, miR-125b, miR-129, miR-130a, miR-133, miR-134, miR-155, U6
Stem Cell-Specific miRNA Plate Assay Kit III  miR-17-92 cluster
Stem Cell-Specific miRNA Plate Assay Kit IV  miR-290 cluster



Cancer miRNA Profiling Plate Assay I

miRNAs have been implicated in the regulation of various biological processes such as cell death and cell proliferation. They can act as oncogenes and tumor suppressor genes, playing a crucial role in tumorogenesis. The change in the expression level of miRNAs is associated with dysfunction of their corresponding protein-coding gene targets, many of which are involved in the initiation and progression of cancer. For example, BCL2 oncogene is targeted by miR-15a and miR-16, while PTEN tumor suppressor is targeted by miR-21, and HOXD10, Pak, MYOD1 and ER, and Her2 are targeted by miR-10b, miR-7, miR-206, and miR-125a, respectively. 
Signosis developed cancer miRNA plate assay kit I for quantitatively profiling the expression of seven well-known cancer related miRNAs, which are miRNA-15a, miR-16, miR-21, miR10b, miR-7, miR-125a and miR206. RNU48 is included for normalization.

 

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