Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :11322385
Publication Date : //

Alternative activation of extracellular signal-regulated protein kinases in curcumin and arsenite-induced HSP70 gene expression in human colorectal carcinoma cells.


We have investigated the regulation mechanism of chemical stress-induced HSP70 gene expression in human colorectal carcinoma cells (COLO205 and HT29). Our data show that chemical treatments including sodium arsenite and curcumin, induced significant synthesis of HSP70 and its mRNA. The induced HSP70 gene expression appears to be increased at the transcriptional level. The increase in HSP70 gene expression by both chemicals is associated with an increase in HSF binding to HSE and induction of HSF1 di- or trimerization. Phosphorylation and activation of extracellular signal-regulated proteins (ERK1/2) were detected in sodium arsenite-treated COLO205 and HT29 cells, and the free radical scavenger N-acetyl-L-cysteine (NAC) was able to inhibit this ERK1/2 activation and HSP70 gene expression. MAPK blockade by the specific MEK1 inhibitor (PD98059) decreased the ability of sodium arsenite to increase HSP70 gene expression in a dose-dependent manner along with dephosphorylation of ERK1/2 proteins. In contrast to arsenite treatment, activation of ERK1/2 was not detected in curcumin-treated colorectal carcinoma cells, and NAC and PD98059 did not show any inhibitory effect on HSP70 gene expression induced by curcumin. Overexpression of a dominant negative mutant of mitogen-activated protein kinase kinase kinase 1 (MEKK1-DN) prevents arsenite-induced ERK1/2 phosphorylation and HSP70 protein synthesis. These results indicated that the ERK signaling pathway can participate in HSP70 gene expression induced by the prooxidant sodium arsenite, but not by the antioxidant curcumin.

Authors : Chen Y C , Tsai S H , Shen S C , Lin J K , Lee W R ,

Related products :

Catalog number Product name Quantity
32-203 The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks). The sequential activation of individual members of this family and 0.1 mL
32-202 The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks). The sequential activation of individual members of this family and 0.1 mL
EIAAB24877 ERK7,ERK-7,ERK8,ERK-8,Extracellular signal-regulated kinase 7,Extracellular signal-regulated kinase 8,Homo sapiens,Human,MAP kinase 15,MAPK 15,MAPK15,Mitogen-activated protein kinase 15
27-769 PIAS4 is an inhibitor of TRIF-induced ISRE and NF-kappaB activation. Its mRNA is selectively expressed in endothelial cells, and its expression can be regulated by angiogenic growth factors 0.05 mg
18-003-42916 Small inducible cytokine A18 - CCL18; Macrophage inflammatory protein 4; MIP-4; Pulmonary and activation-regulated chemokine; CC chemokine PARC; Alternative macrophage activation-associated CC chemoki 0.05 mg Aff Pur
10-663-45029 Macrophage Inflammatory Protein-4 (MIP-4) Human - CCL18; Macrophage inflammatory protein 4; MIP-4; Pulmonary and activation-regulated chemokine; CC chemokine PARC; Alternative macrophage activation-as 0.002 mg
10-663-45029 Macrophage Inflammatory Protein-4 (MIP-4) Human - CCL18; Macrophage inflammatory protein 4; MIP-4; Pulmonary and activation-regulated chemokine; CC chemokine PARC; Alternative macrophage activation-as 0.01 mg
10-663-45029 Macrophage Inflammatory Protein-4 (MIP-4) Human - CCL18; Macrophage inflammatory protein 4; MIP-4; Pulmonary and activation-regulated chemokine; CC chemokine PARC; Alternative macrophage activation-as 1 mg
EIAAB27645 Homo sapiens,Human,Nasopharyngeal carcinoma down-regulated gene protein 1,Nasopharyngeal carcinoma-related gene protein,NPCDR1,NPCDRG,NPCR
PY-10188 Signal transduction related antibodies: Anti- Extracellular signal-regulated kinase 3(ERK3),Chicken-Poly 100μg
PY-10201 Signal transduction related antibodies: Anti- Extracellular signal-regulated kinase 1(ERK1),Chicken-Poly 100μg
E01E0171 Human Extracellular Signal Regulated Kinase 96 Tests/kit
27-335 Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The MXI1 gene encodes a transcripti 0.05 mg
31-254 Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The MXI1 gene encodes a transcripti 0.05 mg
bs-0996P Peptides: ERK(Extracellular signal-regulated kinase) Protein Length:12-25 amino acids. 200ug lyophilized
E01E0058 Human Extracellular Signal Regulated Kinase 1 ELISA 96T/kit
201-12-0841 Human extracellular signal-regulated kinase(ERK)ELISA Kit 96T
YHB1166Hu Human extracellular signal-regulated kinase,ERK ELISA Kit 96T
E01E0171 Human Extracellular Signal Regulated Kinase ELISA 96T/kit
E01E0062 Human Extracellular signal Regulated Kinase1 2 ELISA 96T/kit
E01E0059 Human Extracellular Signal Regulated Kinase 2 ELISA 96T/kit
E01E0171 Human Extracellular Signal Regulated Kinase ELISA, ERK
E8989h Human Extracellular Signal Regulated Kinase 2 ELIS 96T
E9174h Human Extracellular Signal Regulated Kinase 1 ELIS 96T
201-12-0841 Human extracellular signal-regulated kinase,ERK ELISA Kit 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur