Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :11591788
Publication Date : //

High-dose leptin activates human leukocytes via receptor expression on monocytes.


Leptin is capable of modulating the immune response. Proinflammatory cytokines induce leptin production, and we now demonstrate that leptin can directly activate the inflammatory response. RNA expression for the leptin receptor (Ob-R) was detectable in human PBMCs. Ob-R expression was examined at the protein level by whole blood flow cytometry using an anti-human Ob-R mAb 9F8. The percentage of cells expressing leptin receptor was 25 +/- 5% for monocytes, 12 +/- 4% for neutrophils, and 5 +/- 1% for lymphocytes (only B lymphocytes). Incubation of resting PBMCs with leptin induced rapid expression of TNF-alpha and IL-6 mRNA and a dose-dependent production of TNF-alpha and IL-6 by monocytes. Incubation of resting PBMCs with high-dose leptin (250 ng/ml, 3-5 days) induced proliferation of resting cultured PBMCs and their secretion of TNF-alpha (5-fold), IL-6 (19-fold), and IFN-gamma (2.5-fold), but had no effect on IL-4 secretion. The effect of leptin was distinct from, and additive to, that seen after exposure to endotoxin or activation by the mixed lymphocyte reaction. In conclusion, Ob-R is expressed on human circulating leukocytes, predominantly on monocytes. At high doses, leptin induces proinflammatory cytokine production by resting human PBMCs and augments the release of these cytokines from activated PBMCs in a pattern compatible with the induction of Th1 cytokines. These results demonstrate that leptin has a direct effect on the generation of an inflammatory response. This is of relevance when considering leptin therapy and may partly explain the relationship among leptin, proinflammatory cytokines, insulin resistance, and obesity.

Authors : Zarkesh-Esfahani H , Pockley G , Metcalfe R A , Bidlingmaier M , Wu Z , Ajami A , Weetman A P , Strasburger C J , Ross R J ,

Related products :

Catalog number Product name Quantity
orb80514 Human LeukinFeron protein A single dose of Human LeukinFeron is prodced from 1million leukocytes isolated from doner's blood contains natural IFN-alpha 10,000IU and complex of cytokines from the first 10,000 IU
K2-BFU2-4HH HALO-Tox HT, GFS: EPO (high dose), Population: BFU-E 2, Human 4 Plate Kits
K2-BFU2-2HH HALO-Tox HT, GFS: EPO (high dose), Population: BFU-E 2, Human 2 Plate Kits
25-938 The erythropoietin receptor is a member of the cytokine receptor family. Upon erythropoietin binding, the erythropoietin receptor activates Jak2 tyrosine kinase which activates different intracellular 0.05 mg
K3-BFU2-2HH HALO-384 Tox Assay Kits, GFS: EPO (high dose), Population: BFU-E 2, Human 2 Plate Kits
K3-BFU2-4HH HALO-384 Tox Assay Kits, GFS: EPO (high dose), Population: BFU-E 2, Human 4 Plate Kits
K2-BFU2-2CH HALO-Tox HT, GFS: EPO (high dose), Population: BFU-E 2, Dog 2 Plate Kits
K2-BFU2-4CH HALO-Tox HT, GFS: EPO (high dose), Population: BFU-E 2, Dog 4 Plate Kits
K2-BFU2-4RH HALO-Tox HT, GFS: EPO (high dose), Population: BFU-E 2, Rat 4 Plate Kits
K2-BFU2-2RH HALO-Tox HT, GFS: EPO (high dose), Population: BFU-E 2, Rat 2 Plate Kits
K2-BFU2-4PrH HALO-Tox HT, GFS: EPO (high dose), Population: BFU-E 2,NHP 4 Plate Kits
K2-BFU2-4MH HALO-Tox HT, GFS: EPO (high dose), Population: BFU-E 2, Mouse 4 Plate Kits
K2-BFU2-2MH HALO-Tox HT, GFS: EPO (high dose), Population: BFU-E 2, Mouse 2 Plate Kits
K2-BFU2-2PrH HALO-Tox HT, GFS: EPO (high dose), Population: BFU-E 2,NHP 2 Plate Kits
K3-BFU2-2RH HALO-384 Tox Assay Kits, GFS: EPO (high dose), Population: BFU-E 2, Rat 2 Plate Kits
K3-BFU2-4CH HALO-384 Tox Assay Kits, GFS: EPO (high dose), Population: BFU-E 2, Dog 4 Plate Kits
K3-BFU2-4RH HALO-384 Tox Assay Kits, GFS: EPO (high dose), Population: BFU-E 2, Rat 4 Plate Kits
K3-BFU2-2CH HALO-384 Tox Assay Kits, GFS: EPO (high dose), Population: BFU-E 2, Dog 2 Plate Kits
K3-BFU2-2MH HALO-384 Tox Assay Kits, GFS: EPO (high dose), Population: BFU-E 2, Mouse 2 Plate Kits
K3-BFU2-2PrH HALO-384 Tox Assay Kits, GFS: EPO (high dose), Population: BFU-E 2, NHP 2 Plate Kits
K3-BFU2-4PrH HALO-384 Tox Assay Kits, GFS: EPO (high dose), Population: BFU-E 2, NHP 4 Plate Kits
K3-BFU2-4MH HALO-384 Tox Assay Kits, GFS: EPO (high dose), Population: BFU-E 2, Mouse 4 Plate Kits
27-929 NFE2L3 activates erythroid-specific, globin gene expression. 0.05 mg
K1007-1 Leptin Receptor ELISA Kit, Human Detect total serum leptin receptor using a highly specific and sensitive monoclonal antibody. Detection is performed with a horseradish peroxidase (HRP)_conjugated sec 1 kit
orb80512 Human Leptin Binding Domain protein Leptin Binding Domain Human Recombinant also called Leptin soluble receptor produced in E.coli is a single, non-glycosylated, polypeptide chain containing 208 amino 10


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur