Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :11941469
Publication Date : //

Daylength and spatial expression of a gibberellin 20-oxidase isolated from hybrid aspen (Populus tremula L. x P. tremuloides Michx.).


Physiologically active gibberellins (GAs) are key regulators of shoot growth in trees. To investigate this mechanism of GA-controlled growth in hybrid aspen, we cloned cDNAs encoding gibberellin 20-oxidase (GA 20-oxidase), a key, highly regulated enzyme in the biosynthesis of GAs. Clones were isolated from leaf and cambium cDNA libraries using probes generated by polymerase chain reaction, based on conserved domains of GA 20-oxidases. Upon expression in Escherichia coli, the GST-fusion protein was shown to oxidise GA12 as well as oxidising the 13-hydroxylated substrate GA53, successively to GA9 and GA20, respectively. The gene PttGA20ox1 was expressed in meristematic cells and growing tissues such as expanding internodes, leaves and roots. The expression was negatively regulated by both GA4 and overexpression of phytochrome A. RNA analysis also showed that the expression was down-regulated in late-expanding leaf tissue in response to short days (SDs). Actively growing tissues such as early elongating internodes, petioles and leaf blades had the highest levels of C19-GAs. Upon transfer to SDs an accumulation of GA19 was observed in early elongating internodes and leaf blades. The levels of C19-GAs were also to some extent changed upon transfer to SDs. The levels of GA20 were down-regulated in internodes, and those of GA1 were significantly reduced in early expanding leaf blades. In roots the metabolites GA19 and GA8 decreased upon shifts to SDs, while GA20 accumulated slightly. The down-regulation of GA 20-oxidase activity in response to SDs was further indicated by studies of [14C]GA12 metabolism in shoots, demonstrating that the substrate for GA 20-oxidase, [14C]GA53, accumulates in SDs.

Authors : Eriksson Maria E , Moritz Thomas ,

Related products :

Catalog number Product name Quantity
AS05 080 Antibody: Xet | xyloglucan endotransglucosylase, Immunogen: recombinant Xet protein derived from Pupulus tremula x tremuloides sequence A2TEJ3, Host: rabbit, polyclonal, Confirmed reactivity: Populus 200
SPATL_HUMAN ELISA Kit FOR Protein SPATIAL; organism: Human; gene name: SPATIAL 96T
SPATL_MOUSE ELISA Kit FOR Protein SPATIAL; organism: Mouse; gene name: Spatial 96T
EIAAB39422 C10orf27,Homo sapiens,Human,Protein SPATIAL,SPATIAL,Stromal protein associated with thymii and lymph node homolog
EIAAB39423 Bos taurus,Bovine,Protein SPATIAL,SPATIAL,Stromal protein associated with thymii and lymph node homolog
EIAAB39421 Mouse,Mus musculus,Protein SPATIAL,Spatial,Stromal protein associated with thymii and lymph node,Tbata
27-341 The POU6F1 gene encodes a protein that is part of a family of transcription factors which exhibit distinct temporal and spatial patterns of expression. 0.05 mg
30-298 The POU6F1 gene encodes a protein that is part of a family of transcription factors which exhibit distinct temporal and spatial patterns of expression. 0.05 mg
26-241 The POU6F1 gene encodes a protein that is part of a family of transcription factors which exhibit distinct temporal and spatial patterns of expression. 0.05 mg
301-992 Spin column for Hybrid-R (type B) w_ 2ml collection tube, Accessories for Hybrid-R 10ea x 10
301-992 Spin column for Hybrid-R (type B) w 2ml collection tube, Accessories for Hybrid-R 10ea x 10
32-166 Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part 0.1 mg
303-990 Spin column for Hybrid-R Blood RNA (type W) w_ 2ml collection tube, Accessories for Hybrid-R Blood RNA 10ea x 5
303-990 Spin column for Hybrid-R Blood RNA (type W) w 2ml collection tube, Accessories for Hybrid-R Blood RNA 10ea x 5
306-990 Spin column for Hybrid-R miRNA (type B) w_ 2ml collection tube, Accessories for Hybrid-R miRNA 10ea x 5
306-990 Spin column for Hybrid-R miRNA (type B) w 2ml collection tube, Accessories for Hybrid-R miRNA 10ea x 5
306-990 Spin column for Hybrid-R miRNA (type W) w_ 2ml collection tube, Accessories for Hybrid-R miRNA 10ea x 5
306-990 Spin column for Hybrid-R miRNA (type W) w 2ml collection tube, Accessories for Hybrid-R miRNA 10ea x 5
30-228 Sterol-C4-mehtyl oxidase-like protein was isolated based on its similarity to the yeast ERG25 protein. It contains a set of putative metal binding motifs with similarity to that seen in a family of me 0.1 mg
100-150 Exprep Hybrid-Q Plasmid , ExprepTM Hybrid-Q Plasmid Rapidprep, mini 1 kit, 50
468-44-0 Gibberellin A4 GA4 1g
510-75-8 Gibberellin A7 GA7 1g
16627-04 Gibberellin A3 100MG
16627-91 Gibberellin A3 CAS: 77-06-5 1G
16627-04 Gibberellin A3 CAS: 77-06-5 100MG


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur