Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :12957182
Publication Date : //

Development of a measles specific IgM ELISA for use with serum and oral fluid samples using recombinant measles nucleoprotein produced in Saccharomyces cerevisiae.


In order to develop sensitive assays for detecting measles antibodies in oral fluid specimens, we have produced recombinant measles virus nucleoprotein (rMVN) in a yeast expression system and prepared monoclonal antibodies to the protein. Measles nucleoprotein gene from the Schwarz vaccine strain was cloned into a yeast expression vector, pFX7 under the control of the hybrid GAL10-PYK1 promoter. High levels of rMVN (20 mg/litre of yeast culture) were generated. Electron microscopy showed that the purified rMVN assembled into typical herring-bone structures. Monoclonal antibodies produced to the rMVN also reacted with native measles virus N in immunofluorescence tests. The purified rMVN and a monoclonal antibody to the rMVN conjugated to horseradish peroxidase were used to develop a measles specific IgM capture EIA (MACEIA) in both serum and oral fluid specimens. Evaluations of the MACEIA were performed by testing a) serum samples (n=80) and b) paired oral fluid/serum samples from measles cases (n=50, representing 16 cases) and oral fluids from controls with non-measles rash (n=59, representing 48 cases). The samples were also tested for measles IgM, using a reference radioimmunoassay (MACRIA). The sensitivity and specificity of the MACEIA compared with MACRIA for a) the serum samples were 100 and 96.6% respectively and b) for paired serum/oral fluids samples 100 and 100%, respectively.

Authors : Samuel Dhanraj , Sasnauskas Kestutis , Jin Li , Gedvilaite Alma , Slibinskas Rimas , Beard Stuart , Zvirbliene Aurelija , Oliveira Solange Artimos , Staniulis Juozas , Cohen Bernard , Brown David ,

Related products :

Catalog number Product name Quantity
RP-1613 Recombinant (E. Coli) purified Measles virus Large Polymerase (58-149) Species Reactivity: Measles 100 ug Product tipe: Pure protein
RP-1611 Recombinant (E. Coli) purified Measles virus nucleocapsid protein (89-165) Species Reactivity: Measles 100 ug Product tipe: Pure protein
RP-1609 Recombinant (E. Coli) purified Measles virus hemagglutinin immunodominant mosaic (106-114+519-550) protein Species Reactivity: Measles 100 ug Product tipe: Pure protein
RP-1612 Recombinant (E. Coli) purified Measles virus Large Polymerase (2059-2183) Species Reactivity: Measles 100 ug Product tipe: Pure protein
RP-1610 Recombinant (E. Coli) purified Measles virus hemagglutinin immunodominant region (399-525) protein Species Reactivity: Measles 100 ug Product tipe: Pure protein
mev-002 Recombinant Measles Virus Non-Structural C-Protein (1-51) Measles (1-51) 1000
mev-002 Recombinant Measles Virus Non-Structural C-Protein (1-51) Measles (1-51) 500
mev-002 Recombinant Measles Virus Non-Structural C-Protein (1-51) Measles (1-51) 100
18-70000 Antigens Measles Virus Nucleoprotein, Recombinant 20
RP-1614 Recombinant (E. Coli) purified Measles virus Non-Structural C-Protein immunodominant region (1-51aa) Species Reactivity: Measles 100 ug Product tipe: Pure protein
EV9298 Rubeola (measles) Virus Nucleoprotein, Recombinant host: sf9 Insect Cells 1mg
mev-003 Recombinant Measles Virus Large Polymerase (2059-2183) VIRAL ANTIGENS- MEASLES VIRUS 100
mev-005 Recombinant Measles Virus Hemagglutinin Mosaic (1-30115-150379-410) VIRAL ANTIGENS- MEASLES VIRUS 1mg
mev-003 Recombinant Measles Virus Large Polymerase (2059-2183) VIRAL ANTIGENS- MEASLES VIRUS 0.5mg
mev-005 Recombinant Measles Virus Hemagglutinin Mosaic (1-30115-150379-410) VIRAL ANTIGENS- MEASLES VIRUS 0.5mg
mev-005 Recombinant Measles Virus Hemagglutinin Mosaic (1-30115-150379-410) VIRAL ANTIGENS- MEASLES VIRUS 100
mev-003 Recombinant Measles Virus Large Polymerase (2059-2183) VIRAL ANTIGENS- MEASLES VIRUS 1mg
mev-006 Recombinant Measles Virus (399-525) Measles (399-525) 1000
mev-006 Recombinant Measles Virus (399-525) Measles (399-525) 500
mev-006 Recombinant Measles Virus (399-525) Measles (399-525) 100
mev-007 Recombinant Measles Virus Fusion Mosaic (106-114519-550) VIRAL ANTIGENS- MEASLES VIRUS 0.5mg
mev-007 Recombinant Measles Virus Fusion Mosaic (106-114519-550) VIRAL ANTIGENS- MEASLES VIRUS 100
mev-007 Recombinant Measles Virus Fusion Mosaic (106-114519-550) VIRAL ANTIGENS- MEASLES VIRUS 1mg
mev-001 Recombinant Measles Virus Large Polymerase (58-149) VIRAL ANTIGENS- MEASLES VIRUS 1mg
mev-002 Recombinant Measles Virus Non-Structural C-Protein (1-51) VIRAL ANTIGENS- MEASLES VIRUS 1mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur