Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :12966098
Publication Date : //

Defective human MutY phosphorylation exists in colorectal cancer cell lines with wild-type MutY alleles.

Oxidative DNA damage can generate a variety of cytotoxic DNA lesions such as 8-oxoguanine (8-oxoG), which is one of the most mutagenic bases formed from oxidation of genomic DNA because 8-oxoG can readily mispair with either cytosine or adenine. If unrepaired, further replication of A.8-oxoG mispairs results in C:G to A:T transversions, a form of genomic instability. We reported previously that repair of A.8-oxoG mispairs was defective and that 8-oxoG levels were elevated in several microsatellite stable human colorectal cancer cell lines lacking MutY mutations (human MutY homolog gene, hmyh, MYH MutY homolog protein). In this report, we provide biochemical evidence that the defective repair of A.8-oxoG may be due, at least in part, to defective phosphorylation of the MutY protein in these cell lines. In MutY-defective cell extracts, but not extracts with functional MutY, A.8-oxoG repair was increased by incubation with protein kinases A and C (PKA and PKC) and caesin kinase II. Treatment of these defective cells, but not cells with functional MutY, with phorbol-12-myristate-13-acetate also increased the cellular A.8-oxoG repair activity and decreased the elevated 8-oxoG levels. We show that MutY is serine-phosphorylated in vitro by the action of PKC and in the MutY-defective cells by phorbol-12-myristate-13-acetate but that MutY is already phosphorylated at baseline in proficient cell lines. Finally, using antibody-isolated MutY protein, we show that MutY can be directly phosphorylated by PKC that directly increases the level of MutY catalyzed A.8-oxoG repair.

Authors : Parker Antony R , O'Meally Robert N , Sahin Fikret , Su Gloria H , Racke Frederick K , Nelson William G , DeWeese Theodore L , Eshleman James R ,

Related products :

Catalog number Product name Quantity
CSB-EL015245HU Human mutY homolog (E. coli) (MUTYH) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL015245RA Rat mutY homolog (E. coli) (MUTYH) ELISA kit, Species Rat, Sample Type serum, plasma 96T
MUTY11-S Anti-Human MutY Homolog ab #1 100 ul
MUTY11-A Anti-Human MutY IgG #1, aff pure 100 ug
MUTY11-P Human MutY Control_blocking peptide 1 100 ug
E14239h Human MutY Homolog ELISA Kit 96T
201-12-2943 Human MutY Homolog (MUTYH)ELISA Kit 96T
YHB2077Hu Human MutY Homolog (MUTYH)ELISA Kit 48T
QY-E04792 Human MutY Homolog(MUTYH)ELISA Kit 96T
MUTY12-P Human MutY homolog Control_blocking peptide 2 100 ug
E2942Hu Human MutY Homolog (MUTYH)ELISA Kit 96T
20389 Human MutY Homolog (MUTYH) ELISA Kit 96T
MUTY11-P Human MutY Control blocking peptide #1 100 ug
MUTY12-S Anti-Human MutY homolog peptide ab #2 100 ul
UT-E04792 Human MutY Homolog (MUTYH) ELISA Kit 96T
E2941Hu Human MutY Homolog (MUTYH)ELISA Kit 48T
201-12-2943 Human MutY Homolog(MUTYH)ELISA Kit 96T
YHB2077Hu Human MutY Homolog (MUTYH)ELISA Kit 96T
E2942Hu Human MutY Homolog (MUTYH)ELISA Kit 48T
UB-E04792 Human MutY Homolog(MUTYH)ELISA Kit 96T
201-12-2943 Human MutY Homolog (MUTYH)ELISA Kit 48T
E13651256 Human MutY Homolog (MUTYH) ELISA Kit 1
MUTY12-A Anti-Human MutY homolog peptide IgG #2, aff. Pure 100 ug
MUTY12-P Human MutY homolog Control blocking peptide #2 100 ug
MUTY11-C Recombinant Human MutY Protein for Western blot 100 ul


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017


france@gentaur.com | Gentaur

Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

spain@gentaur.com | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur