Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :1347664
Publication Date : //

A single base change at a splice acceptor site leads to a truncated CAD protein in Urd-A mutant Chinese hamster ovary cells.


We have previously reported the isolation and characterization of mutant Chinese hamster ovary (CHO-K1) cells of the Urd-A complementation group, which require uridine for growth, are deficient in the activities of the first three enzymes of de novo UMP biosynthesis, and produce markedly reduced amounts of a truncated form of the multifunctional protein CAD, which contains these three enzyme activities. We report here that a single base change of G to A at a highly conserved RNA splice acceptor site is responsible for the phenotype of this mutant. In addition to a small amount of apparently normal CAD mRNA, this mutation causes production of two alternative forms of CAD mRNA in the mutant, one that includes the intron just prior to the mutation and one that excludes the exon just after the mutation. The affected splice site is located at the intron-exon boundary just preceding the exon that encodes the beginning of the aspartate transcarbamylase (ATCase) domain of the CAD protein. Both intron inclusion and exon exclusion during RNA processing introduce a translation stop codon upstream of the region encoding this domain, resulting in the production of the truncated CAD protein seen in the Urd-A mutant. This mutation also results in markedly decreased levels of CAD mRNA and protein in the mutant.

Authors : Patterson D , Berger R , Bleskan J , Vannais D , Davidson J ,

Related products :

Catalog number Product name Quantity
32-195 The TUG protein contains a UBX domain, for GLUT4. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 0.1 mL
PR-694 BMP-7 CHO Bone Morphogenetic Protein-7 human, recombinant, Chinese hamster ovary (CHO) cells 10
PR-694 BMP-7 CHO Bone Morphogenetic Protein-7human, recombinant, Chinese hamster ovary (CHO) cells 10
PR-694 Proteins: BMP-7 CHO Bone Morphogenetic Protein-7human, recombinant, Chinese hamster ovary (CHO) cells 10
PR-402 EPO alpha Erythropoietin alphahuman, recombinant, chinese hamster ovary cells (CHO cells) 50
PR-402 Proteins: EPO alpha Erythropoietin alphahuman, recombinant, chinese hamster ovary cells (CHO cells) 50
orb80019 Human Erythropoietin-alpha protein Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 165 a 500 IU
PR-470 IL-10 Interleukin 10human, recombinant, Chinese Hamster Ovary (CHO) cells 10
PR-234 Il-12 Interleukin 12 human, recombinant, Chinese Hamster Ovary (CHO) cells 10
PR-470 IL-10 Interleukin 10 human, recombinant, Chinese Hamster Ovary (CHO) cells 10
PR-234 Il-12 Interleukin 12human, recombinant, Chinese Hamster Ovary (CHO) cells 10
PR-234 Il_12 Interleukin 12 human, recombinant, Chinese Hamster Ovary (CHO) cells 10 µg
PR-470 IL_10 Interleukin 10 human, recombinant, Chinese Hamster Ovary (CHO) cells 10 µg
PR-470 Proteins: IL-10 Interleukin 10human, recombinant, Chinese Hamster Ovary (CHO) cells 10
PR-234 Il_12 Interleukin 12 human, recombinant, Chinese Hamster Ovary (CHO) cells 10µg
PR-470 IL_10 Interleukin 10 human, recombinant, Chinese Hamster Ovary (CHO) cells 10µg
PR-234 Proteins: Il-12 Interleukin 12human, recombinant, Chinese Hamster Ovary (CHO) cells 10
PR-424 G-CSF Granulocyte-Colony Stimulating Factorhuman, recombinant, Chinese Hamster Ovary (CHO) cells 10
PR-694 BMP_7 CHO Bone Morphogenetic Protein_7 human, recombinant, Chinese hamster ovary (CHO) cells 10µg
PR-694 BMP_7 CHO Bone Morphogenetic Protein_7 human, recombinant, Chinese hamster ovary (CHO) cells 10 µg
PR-424 G-CSF Granulocyte-Colony Stimulating Factor human, recombinant, Chinese Hamster Ovary (CHO) cells 10
PR-424 G_CSF Granulocyte_Colony Stimulating Factor human, recombinant, Chinese Hamster Ovary (CHO) cells 10 µg
PR-424 Proteins: G-CSF Granulocyte-Colony Stimulating Factorhuman, recombinant, Chinese Hamster Ovary (CHO) cells 10
PR-424 G_CSF Granulocyte_Colony Stimulating Factor human, recombinant, Chinese Hamster Ovary (CHO) cells 10µg
PR-409 NGF beta Nerve Growth Factor betahuman, recombinant, Chinese hamster ovary (CHO) cells 20


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur