Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :14662315
Publication Date : //

Purification and properties of an aminopeptidase from the mid-gut gland of scallop (Patinopecten yessoensis).


An aminopeptidase was isolated from the mid-gut gland of Patinopecten yessoensis. The enzyme was purified from an acetone-dried preparation by extracting, ammonium sulfate precipitation, Hi-Load Q column chromatography, isoelectric focusing, and POROS HP2 and HQ column chromatography. The molecular weight of the enzyme was estimated to be 61 kDa by SDS-polyacrylamide gel electrophoresis and 59 kDa by gel permeation chromatography. The isoelectric point of the enzyme was 5.2 and the optimum pH was 7.0 toward leucine p-nitroanilide (Leu-pNA). The enzyme was inhibited by o-phenanthroline. The activity of the enzyme treated with o-phenanthroline was completely recovered by adding excess Zn(2+). Relative hydrolysis rates of amino acid-pNAs and amino acid-4-methylcoumaryl-7-amides (amino acid-MCAs) indicated that the enzyme preferred substrates having Ala or Met as an amino acid residue. The enzyme had a K(m) of 32.2 microM and k(cat) of 29.5 s(-1) with Ala-pNA and a K(m) of 11.1 microM and k(cat) of 9.49 s(-1) with Ala-MCA. The enzyme sequentially liberated amino acids from the amino-termini of Ala-Phe-Tyr-Glu.

Authors : Umetsu Hironori , Arai Mito , Ota Toshinori , Kudo Ryohei , Sugiura Hitomi , Ishiyama Hiroyuki , Sasaki Kazuo ,

Related products :

Catalog number Product name Quantity
EIAAB46562 Aminoacylproline aminopeptidase,App,Cytosolic aminopeptidase P,Rat,Rattus norvegicus,sAmp,Soluble aminopeptidase P,Xaa-Pro aminopeptidase 1,Xpnpep1,X-Pro aminopeptidase 1,X-prolyl aminopeptidase 1, so
E0536b ELISA Bos taurus,Bovine,Cytosol aminopeptidase,LAP3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Peptidase S,Proline aminopeptidase,Prolyl aminopeptidase 96T
E0536m ELISA Cytosol aminopeptidase,Lap3,LAP-3,Lapep,Leucine aminopeptidase 3,Leucyl aminopeptidase,Mouse,Mus musculus,Proline aminopeptidase,Prolyl aminopeptidase 96T
U0536m CLIA Cytosol aminopeptidase,Lap3,LAP-3,Lapep,Leucine aminopeptidase 3,Leucyl aminopeptidase,Mouse,Mus musculus,Proline aminopeptidase,Prolyl aminopeptidase 96T
U0536b CLIA Bos taurus,Bovine,Cytosol aminopeptidase,LAP3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Peptidase S,Proline aminopeptidase,Prolyl aminopeptidase 96T
E0536b ELISA kit Bos taurus,Bovine,Cytosol aminopeptidase,LAP3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Peptidase S,Proline aminopeptidase,Prolyl aminopeptidase 96T
E0536m ELISA kit Cytosol aminopeptidase,Lap3,LAP-3,Lapep,Leucine aminopeptidase 3,Leucyl aminopeptidase,Mouse,Mus musculus,Proline aminopeptidase,Prolyl aminopeptidase 96T
E0536r ELISA Cytosol aminopeptidase,Lap3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Proline aminopeptidase,Prolyl aminopeptidase,Rat,Rattus norvegicus 96T
E0536r ELISA kit Cytosol aminopeptidase,Lap3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Proline aminopeptidase,Prolyl aminopeptidase,Rat,Rattus norvegicus 96T
U0536r CLIA Cytosol aminopeptidase,Lap3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Proline aminopeptidase,Prolyl aminopeptidase,Rat,Rattus norvegicus 96T
E0536p ELISA Cytosol aminopeptidase,LAP3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Pig,Proline aminopeptidase,Prolyl aminopeptidase,Sus scrofa 96T
E0536p ELISA kit Cytosol aminopeptidase,LAP3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Pig,Proline aminopeptidase,Prolyl aminopeptidase,Sus scrofa 96T
U0536p CLIA Cytosol aminopeptidase,LAP3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Pig,Proline aminopeptidase,Prolyl aminopeptidase,Sus scrofa 96T
EIAAB46563 Aminoacylproline aminopeptidase,Cytosolic aminopeptidase P,Homo sapiens,Human,sAmp,Soluble aminopeptidase P,Xaa-Pro aminopeptidase 1,XPNPEP1,XPNPEPL,XPNPEPL1,X-Pro aminopeptidase 1,X-prolyl aminopepti
E0536h ELISA Cytosol aminopeptidase,Homo sapiens,Human,LAP3,LAP-3,LAPEP,Leucine aminopeptidase 3,Leucyl aminopeptidase,PEPS,Peptidase S,Proline aminopeptidase,Prolyl aminopeptidase 96T
U0536h CLIA Cytosol aminopeptidase,Homo sapiens,Human,LAP3,LAP-3,LAPEP,Leucine aminopeptidase 3,Leucyl aminopeptidase,PEPS,Peptidase S,Proline aminopeptidase,Prolyl aminopeptidase 96T
E0536h ELISA kit Cytosol aminopeptidase,Homo sapiens,Human,LAP3,LAP-3,LAPEP,Leucine aminopeptidase 3,Leucyl aminopeptidase,PEPS,Peptidase S,Proline aminopeptidase,Prolyl aminopeptidase 96T
EIAAB46565 Aminoacylproline aminopeptidase,Cytosolic aminopeptidase P,Mouse,Mus musculus,sAmp,Soluble aminopeptidase P,Xaa-Pro aminopeptidase 1,Xpnpep1,X-Pro aminopeptidase 1,X-prolyl aminopeptidase 1, soluble
EIAAB46564 Aminoacylproline aminopeptidase,Bos taurus,Bovine,Cytosolic aminopeptidase P,sAmp,Soluble aminopeptidase P,Xaa-Pro aminopeptidase 1,XPNPEP1,X-Pro aminopeptidase 1,X-prolyl aminopeptidase 1, soluble
15-288-22303B Xaa-Pro aminopeptidase 1 - EC 3.4.11.9; X-Pro aminopeptidase 1; X-prolyl aminopeptidase 1. soluble; Cytosolic aminopeptidase P; Soluble aminopeptidase P; sAmp; Aminoacylproline aminopeptidase Polyclon 0.1 mg
15-288-22303B Xaa-Pro aminopeptidase 1 - EC 3.4.11.9; X-Pro aminopeptidase 1; X-prolyl aminopeptidase 1. soluble; Cytosolic aminopeptidase P; Soluble aminopeptidase P; sAmp; Aminoacylproline aminopeptidase Polyclon 0.05 mg
EIAAB13157 Adipocyte-derived leucine aminopeptidase,A-LAP,Aminopeptidase PILS,Appils,Arts1,ARTS-1,Endoplasmic reticulum aminopeptidase 1,Erap1,PILS-AP,Puromycin-insensitive leucyl-specific aminopeptidase,Rat,Rat
104-M01 Aminopeptidase P , Host: Mouse, Species: Anti-Rat, Synonyms: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble 100 ug
EIAAB46570 Aminopeptidase P3,APP3,Homo sapiens,Human,Probable Xaa-Pro aminopeptidase 3,XPNPEP3,X-Pro aminopeptidase 3
EIAAB46568 Aminopeptidase P3,APP3,Mouse,Mus musculus,Probable Xaa-Pro aminopeptidase 3,Xpnpep3,X-Pro aminopeptidase 3


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur