Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :14733593
Publication Date : //

Synthesis, characterization, and evaluation of ferrocene-theophylline conjugates for use in electrochemical enzyme immunoassay.


A series of 8-(ferrocenylalkyl)theophylline conjugates were synthesized for evaluation in a homogeneous, competitive electrochemical immunoassay for theophylline with amperometric detection of the ferrocene label at +320 mV. The electrical signal was amplified via redox cycling with the glucose oxidase/glucose system. The resulting catalytic current was strongly inhibited upon binding of the conjugates to anti-theophylline antibodies such that a large excess of theophylline was required to achieve complete reversal leading to an assay with poor sensitivity in the clinical range. A study of the nonspecific interaction of the antibodies with various ferrocene derivatives indicated that this was reduced when a charged functional group was present on the metallocene ring. Consequently, a conjugate was synthesized with a quaternary ammonium group which when incorporated into the assay resulted in improved sensitivity.

Authors : Forrow Nigel J , Foulds Nicola C , Frew Jane E , Law John T ,

Related products :

Catalog number Product name Quantity
HPT1002-0005 Ferrocene NHS Ester (Amine Reactive Electrochemical Reporter: No. HPT1002) - 50 mg
HPT1002-0700 Ferrocene NHS Ester (Amine Reactive Electrochemical Reporter: No. HPT1002)- 5 x 10 mg
HPT1002-0100 Ferrocene NHS Ester (Amine Reactive Electrochemical Reporter: No. HPT1002)- 1g
HPT1002-0500 Ferrocene NHS Ester (Amine Reactive Electrochemical Reporter: No. HPT1002) - 500 mg
HPT1002-0010 Ferrocene NHS Ester (Amine Reactive Electrochemical Reporter: No. HPT1002) - 100 mg
HPT1003 Ferrocene phosphoramidite (Electrochemical Oligonucleotide Label: HPT1003)
0384301000 FERROCENE 98 percent FOR SYNTHESIS (DICYCLOPENTADIENYLIRON), CAS: 102-54-5 1KGS
F-04068 FERROCENE 98 percent (For Synthesis) (Dicylopentadienyliron) CAS: 102-54-5 100 gm
F-04068 FERROCENE 98 percent (For Synthesis) (Dicylopentadienyliron) CAS: 102-54-5 500 gm
0384300100 FERROCENE 98 percent FOR SYNTHESIS (DICYCLOPENTADIENYLIRON), CAS: 102-54-5 5x100GM
30-374 ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosph 0.1 mg
30-375 ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosph 0.1 mg
26-294 ATP5F1 is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosp 0.05 mg
80-IT20 Theophylline 8-BSA, Hapten Conjugates 10 mg
AF-0403-D005.0-001 Ferrocene-amidopentyl carboxylic acid NHS ester (Ferrocene-C6-SE) 5 mg
F-0403-10 Ferrocene-amidopentyl carboxylic acid (Ferrocene-C6), Redox _ Electron Transfer Dyes 10 mg
AF-0402-5 Ferrocene carboxylic acid NHS ester (Ferrocene-SE), Redox _ Electron Transfer Dyes 5 mg
AF-0403-5 Ferrocene-amidopentyl carboxylic acid NHS ester (Ferrocene-C6-SE), Redox _ Electron Transfer Dyes 5 mg
AF-0402-D005.0-001 Ferrocene carboxylic acid NHS ester (Ferrocene-SE) 5 mg
F-0403-D010.0-001 Ferrocene-amidopentyl carboxylic acid (Ferrocene- C6) 10 mg
K3018-HS PGE2 Enzyme Immunoassay High Sensitivity kit (One Plate) The Prostaglandin E2 (PGE2) Immunoassay kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, saliva and tissue cultu 96 rxns
K3003-1 Cortisol Enzyme Immunoassay kit _ 1 Strip plate The Cortisol Immunoassay kit is designed to quantitatively measure cortisol present in dried fecal extracts and in saliva, urine, serum, plasma and tiss 96 rxns
K3018-1 PGE2 Enzyme Immunoassay kit (One Plate) The new Prostaglandin E2 (PGE2) Immunoassay kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, saliva and tissue culture media samp 96 rxns
26-904 Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit a 0.05 mg
K3003-W1 Cortisol Enzyme Immunoassay kit 96 rxns


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur