Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :14963134
Publication Date : //

The matrix protein of Marburg virus is transported to the plasma membrane along cellular membranes: exploiting the retrograde late endosomal pathway.


VP40, the matrix protein of Marburg virus, is a peripheral membrane protein that has been shown to associate with membranes of multivesicular bodies (MVBs) (L. Kolesnikova, H. Bugany, H.-D. Klenk, and S. Becker, J. Virol. 76:1825-1838, 2002). The present study revealed that VP40 is bound to cellular membranes rapidly after synthesis. Time course studies were performed to trace the distribution of VP40 during the course of expression. First, VP40 was homogenously distributed throughout the cytoplasm, although the majority of protein (70%) was already membrane associated. Next, VP40 accumulated in MVBs and in tubular protrusions emerging from MVBs. Finally, VP40 appeared in a patch-like pattern beneath the plasma membrane. These morphological results were supported by iodixanol density gradient analyses. The majority of VP40-positive membranes were first detected comigrating with small vesicles. VP40 was then shifted to fractions containing endosomal marker proteins, and later, to fractions containing plasma membrane marker proteins. Blocking of protein synthesis by use of cycloheximide at the time when VP40 was mainly associated with the small vesicles did not prevent the redistribution of VP40 to the late endosomes and further to the plasma membrane. The inhibition of intracellular vesicular trafficking by monensin significantly reduced the appearance of VP40 at the plasma membrane. In conclusion, we suggest that the transport of the Marburg virus matrix protein VP40 involves its accumulation in MVBs followed by the redistribution of VP40-enriched membrane clusters to the plasma membrane.

Authors : Kolesnikova Larissa , Bamberg Sandra , Berghöfer Beate , Becker Stephan ,

Related products :

Catalog number Product name Quantity
26-028 Various cellular organelles and vesicles are transported along the microtubules in the cytoplasm. Likewise, membrane recycling of the endoplasmic reticulum (ER), Golgi assembly at the microtubule orga 0.05 mg
31-136 Localized to biosynthetic and endosomal compartments, Rab14 is involved in the biosynthetic_recycling pathway between the Golgi and endosomal compartments. 0.05 mg
25-886 The membrane-associated protein ABCB8 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. AB 0.05 mg
25-919 SLC25A21 is a homolog of the S. cerevisiae ODC proteins, mitochondrial carriers that transport C5-C7 oxodicarboxylates across inner mitochondrial membranes. One of the species transported by ODC is 2- 0.05 mg
Y090699 Anti_p80 endosomal membrane protein Monoclonal 100ul
Y090699 Anti-p80 endosomal membrane protein Monoclonal Antibody 100ul
30-105 GPT participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. 0.1 mg
30-104 GPT participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. 0.1 mg
30-103 GPT participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. 0.1 mg
27-225 KIF20A interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B.It may act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles 0.05 mg
SCH-2470-5004 GOAT ANTI CYTOMEGALOVIRUS LATE MATRIX ANTIGEN, Product Type Polyclonal Antibody, Specificity CYTOMEGALOVIRUS LATE MATRIX ANTIGEN, Target Species Viral, Host Goat, Format Purified, Isotypes Polyc 1 ml
2470-5004 GOAT ANTI CYTOMEGALOVIRUS LATE MATRIX ANTIGEN, Product Type Polyclonal Antibody, Specificity CYTOMEGALOVIRUS LATE MATRIX ANTIGEN, Target Species Viral, Host Goat, Format Purified, Isotypes Polyc 1 ml
SCH-OBT1828 MOUSE ANTI MARBURG VIRUS 30kDa, Product Type Monoclonal Antibody, Specificity MARBURG VIRUS, Target Species Viral, Host Mouse, Format Purified, Isotypes IgG1, Applications E, WB, Clone 3541 0.1 mg
OBT1828 MOUSE ANTI MARBURG VIRUS 30kDa, Product Type Monoclonal Antibody, Specificity MARBURG VIRUS, Target Species Viral, Host Mouse, Format Purified, Isotypes IgG1, Applications E, WB, Clone 3541 0.1 mg
MEDCLA298-1 Epstein Barr Virus (EBV), Late Membrane Prot (LMP_1), 60kD, Cls CS1, CS2, CS3, & CS4, Mab anti_; frzn_prfn, IH 1 ml.
MEDCLA298-01 Epstein Barr Virus (EBV), Late Membrane Prot (LMP_1), 60kD, Cls CS1, CS2, CS3, & CS4, Mab anti_; frzn_prfn, IH 0.1 ml.
EIAAB42457 Havcr2,HAVcr-2,Hepatitis A virus cellular receptor 2 homolog,Rat,Rattus norvegicus,T-cell immunoglobulin and mucin domain-containing protein 3,T-cell membrane protein 3,Tim3,TIM-3,Timd3,TIMD-3
20-272-190001 Marburg Virus - Mouse monoclonal [FM214] to Marburg Virus Monoclonal 0.1 mg
20-272-190005 Marburg Virus - Mouse monoclonal [FM46] to Marburg Virus Monoclonal 0.1 mg
20-272-190004 Marburg Virus - Mouse monoclonal [FM44] to Marburg Virus Monoclonal 0.1 mg
20-272-190003 Marburg Virus - Mouse monoclonal [FM32] to Marburg Virus Monoclonal 0.1 mg
20-272-192335 Marburg Virus - Mouse monoclonal [FM213] to Marburg Virus Monoclonal 0.1 mg
20-272-192334 Marburg Virus - Mouse monoclonal [FM12] to Marburg Virus Monoclonal 0.1 mg
20-272-190002 Marburg Virus - Mouse monoclonal [FM215] to Marburg Virus Monoclonal 0.1 mg
20-272-192333 Marburg Virus - Mouse monoclonal [FM11] to Marburg Virus Monoclonal 0.1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur