Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :14991738
Publication Date : 2004/03/01

GPI-anchored aminopeptidase is involved in the acrosome reaction in sperm of the mussel mytilusedulis.


The sperm of the mussel Mytilus had hydrolytic activities against substrates for aminopeptidase. Acrosome reaction (AR) was suppressed in the presence of aminopeptidase substrate, Phe-4-methylcoumaryl-7-amide (MCA), and an aminopeptidase inhibitor, bestatin. Treatment of sperm with phosphatidylinositol-specific phospholipase C (PI-PLC) released aminopeptidase activity from sperm and suppressed AR. These results suggest that the enzyme is located on the sperm surface via glycosylphosphatidylinositol (GPI)-anchor and is involved in the AR. Immunoblot analysis showed that tyrosine residues of 40, 59, 68, and 72 kDa proteins were phosphorylated during induction of the AR. The 40 kDa protein was also recognized by anti-c-Src antibody by immunoblotting. The tyrosine phosphorylation of these proteins was inhibited when sperm were inseminated in the presence of Phe-MCA, and by PI-PLC treatment. Treatment of sperm with tyrosine kinase activator, 9,10-dimethyl-1,2-benzanthracene, induced AR, and its inhibitor, genistein, suppressed AR. These results suggest that tyrosine phosphorylation of 40, 59, 68, and 72 kDa proteins, induced by the interaction of GPI-anchored aminopeptidase with oocyte surface, triggers AR in Mytilus sperm.

Authors : Togo Tatsuru , Morisawa Masaaki ,

Related products :

Catalog number Product name Quantity
27-206 SPATA16 is involved in the formation of sperm acrosome, which implicated its potential role in spermatogenesis and sperm-egg fusion. Defects in SPATA16 are a cause of globozoospermia; also called Roun 0.05 mg
EIAAB37192 Homo sapiens,Human,SAMP14,SPACA4,Sperm acrosomal membrane-associated protein 14,Sperm acrosome membrane-associated protein 4,UNQ3046_PRO9862
EIAAB37184 Homo sapiens,Human,SAMP32,SPACA1,Sperm acrosomal membrane-associated protein 32,Sperm acrosome membrane-associated protein 1
EIAAB37186 Canis familiaris,Canis lupus familiaris,Dog,SPACA3,Sperm acrosome membrane-associated protein 3,Sperm protein reactive with antisperm antibodies,Sperm protein reactive with ASA,SPRASA
EIAAB37187 Cancer_testis antigen 54,CT54,Homo sapiens,Human,LYC3,Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17,Lysozyme-like protein 3,LYZL3,SLLP1,SPACA3,Sperm acrosome membrane-associated pro
PM-305S Polymerase chain reaction PCR or reverse transcriptase polymerase chain reaction RT PCR primers: Anchored Oligo (dT)20, S pack 100 200
PM-305L Polymerase chain reaction PCR or reverse transcriptase polymerase chain reaction RT PCR primers: Anchored Oligo (dT)20, L pack 100 1ml
EIAAB37188 Lyc3,Lysozyme-like protein 3,Lyzl3,Mouse,mSLLP1,Mus musculus,Sllp1,Spaca3,Sperm acrosome membrane-associated protein 3,Sperm lysozyme-like protein 1
EIAAB46562 Aminoacylproline aminopeptidase,App,Cytosolic aminopeptidase P,Rat,Rattus norvegicus,sAmp,Soluble aminopeptidase P,Xaa-Pro aminopeptidase 1,Xpnpep1,X-Pro aminopeptidase 1,X-prolyl aminopeptidase 1, so
E13121h Human Sperm Acrosome Associated Protein 5 ELISA Ki 96T
E13122h Human Sperm Acrosome Associated Protein 1 ELISA Ki 96T
E13123h Human Sperm Acrosome Associated Protein 4 ELISA Ki 96T
E13124h Human Sperm Acrosome Associated Protein 3 ELISA Ki 96T
H2597 Sperm acrosome-associated protein 5 (SPACA5), Human, ELISA Kit 96T
CSB-EL022454DO Dog Sperm acrosome membrane-associated protein 3(SPACA3) ELISA kit 96T
H2598 Sperm acrosome-associated protein 5 (SPACA5), Mouse, ELISA Kit 96T
CSB-EL022456HU Human Sperm acrosome-associated protein 5(SPACA5) ELISA kit 96T
H2596 Sperm acrosome-associated protein 5 (SPACA5), Bovine, ELISA Kit 96T
CC105_MOUSE Mouse ELISA Kit FOR Sperm acrosome membrane-associated protein 1 96T
CSB-EL022456BO Bovine Sperm acrosome-associated protein 5(SPACA5) ELISA kit 96T
CSB-EL022456MO Mouse Sperm acrosome-associated protein 5(SPACA5) ELISA kit 96T
TMED5_BOVIN Mouse ELISA Kit FOR Sperm acrosome membrane-associated protein 1 96T
E0040h Mouse ELISA Kit FOR Sperm acrosome membrane-associated protein 1 96T
H2590 Sperm acrosome membrane-associated protein 3 (SPACA3), Dog, ELISA Kit 96T
EIAAB37189 Bos taurus,Bovine,SPACA3,Sperm acrosome membrane-associated protein 3


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur