Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :1506668
Publication Date : //

Stereo views and immunogold labeling of the pellicular microtubules at the inner surface of the plasma membrane of Leishmania as revealed by fracture-flip.


We used a modification of fracture-flip to reveal the nanoanatomy of the inner surface of the plasma membrane in promastigotes of Leishmania. After freeze-fracture, lightly fixed promastigotes were coated with a stabilizing layer of carbon evaporated from an electron gun, thawed, and washed. Fractured promastigotes attached to the carbon casts by the protoplasmic (i.e., inner) halves of their plasma membranes were treated with Triton X-100, followed by exposure to low concentrations of trypsin and thorough washing. This was followed by picking up and flipping of the replicas, followed by air-drying. The actual inner surfaces of the plasma membrane were then imaged by platinum shadowing. Extended, three-dimensional, high-resolution views of the inner surface of the plasma membrane showed parallel arrays of microtubules (average spacing 47 nm) closely apposed to the inner surface. Cytochemical labeling confirmed the morphological identification of both subpellicular and flagellar microtubules, as determined by treatment with mouse monoclonal anti-alpha- or anti-beta-tubulin, followed by labeling with goat anti-mouse IgG adsorbed to colloidal gold. Removal of the microtubules revealed parallel arrays of particles (average diameter 17 nm). We hypothesize that these particles represent the cytoplasmic portion of proteins that link the microtubules to the plasma membrane.

Authors : Hou W Y , Pimenta P F , Shen R L , Da Silva P P ,

Related products :

Catalog number Product name Quantity
OBT2004 MOUSE ANTI LEISHMANIA MAJOR SURFACE PROTEASE gp63, Product Type Monoclonal Antibody, Specificity LEISHMANIA MAJOR SURFACE PROTEASE gp63, Target Species Protozoan, Host Mouse, Format Ascites, Isot 0.5 ml
SCH-OBT2004 MOUSE ANTI LEISHMANIA MAJOR SURFACE PROTEASE gp63, Product Type Monoclonal Antibody, Specificity LEISHMANIA MAJOR SURFACE PROTEASE gp63, Target Species Protozoan, Host Mouse, Format Ascites, Isot 0.5 ml
234191 Chiffres & Lettres Noir_Blanc NL-W05-FRC- Fracture - Fraction _ Cijfers & Letters Zwart_Wit NL-W05-FRC - Fracture - Breuken AB Labels;Pack-CD; 78 Pcs Per Pack
74750 IgG,LEISHMANIA MAJOR SURFACE PROTEASE gp63 0.5 ml
AM01176SU-N Leishmania Major Surface Protease (gp63) 0.5 ml
AM01176SU-N Leishmania Major Surface Protease (gp63) 0.5 ml
27-052 EML3 may modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic. 0.05 mg
GWB-B316A1 LEISHMANIA MAJOR SURFACE PROTEASE gp63, Antibody
DS-MB-01777 Mouse Anti-Leishmania Major Surface Protease gp63 500
20-783-74750 MOUSE ANTI LEISHMANIA MAJOR SURFACE PROTEASE GP-63 - Monoclonal 0.5 ml
ABS0185 MOUSE ANTI LEISHMANIA MAJOR SURFACE PROTEASE gp63 0.5 ml
26-116 NUSAP1 is a microtubule-associated protein with the capacity to bundle and stabilize microtubules. It may associate with chromosomes and promote the organization of mitotic spindle microtubules around 0.05 mg
OBT2004 MOUSE ANTI LEISHMANIA MAJOR SURFACE PROTEASE gp63-MONOCLONAL ANTIBODY 0.5 ml
OBT2004 Leishmania Major Surface Protease gp63, Clone 96_126, Mab anti_; IF,ELISA 0.5 ml.
ACLP005A Leishmania major surface protease, GP_63, Clone 96_26, Mab anti_, IF (live & fixed parasites), ELISA, NO WB 0.5 ml.
EIAAB42452 Fracture callus protein 1,FxC1,Fxc1,Mitochondrial import inner membrane translocase subunit Tim9 B,Rat,Rattus norvegicus,Tim10b,Tim9b,TIMM10B,Timm9b
24029 Primary antibody FLIP Antibody, Source Rabbit, Species Reactivity FLIP recognizes all FLIP splice variants including FLIPa, b, and y. 100ug
CSB-EL009085RA Rat fracture callus 1 homolog (rat) (FXC1) ELISA kit, Species Rat, Sample Type serum, plasma 96T
EIAAB42454 Fracture callus protein 1,FxC1,FXC1,Homo sapiens,Human,Mitochondrial import inner membrane translocase subunit Tim9 B,Tim10b,TIM9B,TIMM10B,TIMM9B
HP005 Leishmania Ab Method: Leishmania Ab (different species) Elisa: Parasitic Line 96 t
26-028 Various cellular organelles and vesicles are transported along the microtubules in the cytoplasm. Likewise, membrane recycling of the endoplasmic reticulum (ER), Golgi assembly at the microtubule orga 0.05 mg
OBT2004 MOUSE ANTI LEISHMANIA MAJOR SURFACE PROTEASE gp63 Monoclonal Antibody Host: Mouse IgG2a 0.5 ml
32-114 AURKB (aurora kinase B, AIK2 or aurora-B), with 344-amino acid protein(about 39kDa),localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. AURKB is 0.1 mL
27-223 CAV2 is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism 0.05 mg
25-704 PDZK1 is a scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. It may be involved in the coordina 0.05 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur