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Presence of a novel phosphopentomutase and a 2-deoxyribose 5-phosphate aldolase reveals a metabolic link between pentoses and central carbon metabolism in the hyperthermophilic archaeon Thermococcus kodakaraensis.
Numerous bacteria and mammalian cells harbor two enzymes, phosphopentomutase (PPM) and 2-deoxyribose 5-phosphate aldolase (DERA), involved in the interconversion between nucleosides and central carbon metabolism. In this study, we have examined the presence of this metabolic link in the hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1. A search of the genome sequence of this strain revealed the presence of a closely related orthologue (TK2104) of bacterial DERA genes while no orthologue related to previously characterized PPM genes could be detected. Expression, purification, and characterization of the TK2104 protein product revealed that this gene actually encoded a DERA, catalyzing the reaction through a class I aldolase mechanism. As PPM activity was detected in T. kodakaraensis cells, we partially purified the protein to examine its N-terminal amino acid sequence. The sequence corresponded to a gene (TK1777) similar to phosphomannomutases within COG1109 but not COG1015, which includes all previously identified PPMs. Heterologous gene expression of TK1777 and characterization of the purified recombinant protein clearly revealed that the gene indeed encoded a PPM. Both enzyme activities could be observed in T. kodakaraensis cells under glycolytic and gluconeogenic growth conditions, whereas the addition of ribose, 2-deoxyribose, and 2'-deoxynucleosides in the medium did not lead to a significant induction of these activities. Our results clearly indicate the presence of a metabolic link between pentoses and central carbon metabolism in T. kodakaraensis, providing an alternative route for pentose biosynthesis through the functions of DERA and a structurally novel PPM.
Authors : Rashid Naeem , Imanaka Hiroyuki , Fukui Toshiaki , Atomi Haruyuki , Imanaka Tadayuki ,
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Pathways :
WP1493: Carbon assimilation C4 pathway
WP220: Ribose and Deoxyribose Phosphate Metabolism
WP228: Deoxyribose phosphate metabolism
WP1026: One Carbon Metabolism
WP112: Principle Pathways of Carbon Metabolism
WP1145: One Carbon Metabolism
WP1203: One Carbon Metabolism
WP1230: One Carbon Metabolism
WP1292: One Carbon Metabolism
WP1355: One Carbon Metabolism
WP1657: Glycerolipid metabolism
WP1658: Glycerophospholipid metabolism
WP1664: Inositol phosphate metabolism
WP1671: Methane metabolism
WP1686: Phenylalanine metabolism
WP1770: One carbon metabolism and related pathways
WP2185: Purine metabolism
WP241: One Carbon Metabolism
WP253: Glycolysis
WP260: Glucose-1-phosphate metabolism
WP435: One Carbon Metabolism
WP730: Glutathione and one carbon metabolism
WP796: One Carbon Metabolism
WP909: One Carbon Metabolism
WP100: Glutathione metabolism
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