GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Progestin stimulates the biosynthesis of fibronectin and accumulation of fibronectin mRNA in human endometrial stromal cells.

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :1533646
Publication Date : //

Progestin stimulates the biosynthesis of fibronectin and accumulation of fibronectin mRNA in human endometrial stromal cells.

Fibronectin is a major component of decidual basement membrane. In the present study, we have investigated the effect of progestin on the synthesis and secretion of fibronectin in human endometrial stromal cells. Stromal cells were isolated during the menstrual cycle and cultured in RPMI-1640 with 2% fetal calf serum supplemented with progesterone or medroxyprogesterone acetate (MPA) in a long-term culture system. Indirect immunofluorescent staining showed that fibronectin was uniformly distributed in the intracellular and extracellular regions of stromal cells treated with MPA for 14 days. The biosynthesis and secretion of this protein and the accumulation of cellular fibronectin mRNA were studied after various culture periods. Cells were pulse-labelled with [35S]methionine to determine the amount of newly synthesized fibronectin secreted into the culture medium. A monoclonal antibody (Mab) identified human fibronectin on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), showing a predominant band (Mr 230-250 kDa) which migrated with authentic fibronectin run in parallel. In six endometrial specimens, the amount of radioactivity incorporated as [35S]fibronectin was increased by progestin. Maximal stimulation occurred after 6 days treatment with MPA. Culture beyond 16 days reduced the rate of synthesis and secretion to 40% of the maximum. The effect of progestin was dose dependent with 0.02, 0.2 and 1 microM progesterone, producing 2.0, 3.8 and 11-fold increases respectively, over the control. Medroxyprogesterone acetate was more effective than progesterone, the maximal response (10-fold increase) being achieved at 0.02 microM MPA.(ABSTRACT TRUNCATED AT 250 WORDS)

Authors : Zhu H H , Huang J R , Mazela J , Elias J , Tseng L ,

Related products :

Catalog number	Product name	Quantity

orb81121	Human Fibronectin protein Plasma fibronectin level is elevated in severe coronary artery disease. Increased plasma fibronectin levels are related with venous thromboembolism (VTE) particularly in male	200
IQ417	Mouse Anti-Human Fibronectin Target Antigen Fibronectin Host Isotype Mouse IgG1 Application IHC (P); Immunohistochemical staining of matrix fibronectin in normal and malignant tissues on frozen sec	0.1ml (1mg/ml)
5-81009	Human endometrial stromal cells, female, 43, Irregular bleeding	vial
815	Fibronectin heparin binding 40 kDa fragment from chymotryptic digest of human plasma fibronectin	0.5 mg
815	Fibronectin heparin binding 40 kDa fragment from chymotryptic digest of human plasma fibronectin	5 mg
orb82181	Human Fibronectin protein Fibronectin is a purified protein_bioactive peptide. For research use only.	1 mg
A 4291AG.2	Fibronectin antigen: human cellular Fibronectin	1 mg
A 4291AG.1	Fibronectin antigen: human cellular Fibronectin	250
1107NF-V7079	Monoclonal Antibodies: Fibronectin; reactive species: Human; Clone: : B476; Isotype: IgG1; Specificity: Fibronectin	0.5 ml
A 4290AG.1	Fibronectin antigen: human Fibronectin	250
A 4290AG.2	Fibronectin antigen: human Fibronectin	1 mg
ASHFBN-GF-BIO	FIBRONECTIN POLYCLONAL ANTIBODIES (HOST RABBIT) Rabbit anti human fibronectin IgG fraction, biotin labeled	1.0 mg
ASHFBN-GF-BIO	FIBRONECTIN POLYCLONAL ANTIBODIES (HOST RABBIT) Rabbit anti human fibronectin IgG fraction, biotin labeled	10 mg
ASHFBN-GF-HT	FIBRONECTIN POLYCLONAL ANTIBODIES (HOST RABBIT) Rabbit anti human fibronectin, affinity purified	1.0 mg
ASHFBN-GF-HRP	FIBRONECTIN POLYCLONAL ANTIBODIES (HOST RABBIT) Rabbit anti human fibronectin IgG fraction, HRP labeled	10 mg
ASHFBN-GF-HRP	FIBRONECTIN POLYCLONAL ANTIBODIES (HOST RABBIT) Rabbit anti human fibronectin IgG fraction, HRP labeled	1.0 mg
ASHFBN-GF-HT	FIBRONECTIN POLYCLONAL ANTIBODIES (HOST RABBIT) Rabbit anti human fibronectin, affinity purified	0.1 mg
ASHFBN-GF	FIBRONECTIN POLYCLONAL ANTIBODIES (HOST RABBIT) Rabbit anti human fibronectin IgG fraction	1.0 mg
ASHFBN	FIBRONECTIN POLYCLONAL ANTIBODIES (HOST RABBIT) Rabbit anti human fibronectin antiserum	1.0 ml
ASHFBN-GF	FIBRONECTIN POLYCLONAL ANTIBODIES (HOST RABBIT) Rabbit anti human fibronectin IgG fraction	10 mg
ASHFBN	FIBRONECTIN POLYCLONAL ANTIBODIES (HOST RABBIT) Rabbit anti human fibronectin antiserum	10 ml
HFBN	FIBRONECTIN Human Fibronectin	1.0 mg
HFBN	FIBRONECTIN Human Fibronectin	10 mg
BMDV7079	Fibronectin, connective tissue (NO X w_plasma fibronectin), Clone B476, Mab anti_Human, Mouse; frozen, IH	0.5 ml.
10314-H08H	Fibronectin _ Fibronectin Fragment 2 Protein (His Tag)	200

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel: 0208-080893 Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62 SWIFT RABONL2U

GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur

ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF