Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :15641692
Publication Date : //

Effects of dendritic cells transfected with full length wild type P53 and modified by gastric cancer lysate on immune response.


To investigate the effects of dendritic cells (DCs) transfected with full length wild type p53 and modified by gastric cancer lysates on immune response, the wild type P53 was transducted to DCs with adenovirus, and the DCs were modified by gastric cancer lysates (Lywt-P53DC). The concentration of the surface molecules (B7-1, B7-2, MHC-I , MHC-II) of all DCs was determined by FACS, and the ability of the DCs to induce efficient and specific immunological response in anti-51Cr-labeled target cells studied. BALB/c mice model infected with DCs and Mk28 was established. CTL response in mice immunized with Lywt-p53DC and the effectiveness of Lywt-p53DC in the treatment of tumor-bearing mice was assayed. FACS revealed that the surface molecules of Ly-wt-P53 DC had a high expression: for B7-1 86.70% +/- 0.07%, B7-2 18.77% +/- 0.08%, MHC-I 87.20% +/- 0.05%, MHC-II 56.70%+/-0.07%; The T lymphocytes had a specific CTL lysing ability induced by Lywt-P53DC with the CTL lysis rate being 81%. The immune protective effect of Lywt-p53DC group was more obvious than any other groups (P<0.05). The tumor diameter in Lywt-p53DC group was 3.10+/-0.31 mm, 2.73+/-0.23 mm, 3.70+/-0.07 mm on the day 13, 16 and 19, smaller than DC, wtp53DC and LyDC groups (P<0.05). On the other hand, the growth rate of tumor in Lywt-p53DC group was slower than any other groups (P<0.05). It was suggested that DCs transfected with wild type P53 and modified by gastric cancer lysates had specific CTL killing capability.

Authors : Sun Huawen , Tang Qibing , Chen Yongjun , Tang Cong , Zou ShengQian , Qiu Fazu ,

Related products :

Catalog number Product name Quantity
18-003-43148 NOD-derived CD11c +ve dendritic cells cDNA. RIKEN full-length enriched library. clone F630206P17 product class II transactivator - N_A Polyclonal 0.05 mg Aff Pur
18-003-44305 NOD-derived CD11c +ve dendritic cells cDNA. RIKEN full-length enriched library. clone F630026L17 product regulator of G-protein - N_A Polyclonal 0.1 mg Protein A
5-81011 Human gastric smooth muscle cells (tumor), male, 70, gastric cancer vial
SYN11-R-100 Recombinant purified Human Alpha Synuclein protein (140 aa full length, wild type) 100 ug
SYN12-R-100 Recombinant purified Mouse Alpha Synuclein protein (140 aa full length, wild type) 20 ug
26-760 DCAL1 is a type II transmembrane, C-type lectin-like protein expressed on dendritic cells (DCs) and B cells. It interacts with subsets of T cells as a costimulatory molecule that enhances interleukin- 0.05 mg
26-759 DCAL1 is a type II transmembrane, C-type lectin-like protein expressed on dendritic cells (DCs) and B cells. It interacts with subsets of T cells as a costimulatory molecule that enhances interleukin- 0.05 mg
SCH-MCA1121 MOUSE ANTI HUMAN DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity DENDRITIC CELLS_B CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applications C, Clone 0.2 mg
MCA1121 MOUSE ANTI HUMAN DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity DENDRITIC CELLS_B CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applications C, Clone 0.2 mg
orb82622 Rat IL21 protein IL21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological 2
orb84796 IL21 protein IL21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigenspecific responses of immune cells. The biological effec 10
MCA2494T MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applicatio 25 µg
MCA2494 MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applicatio 0.2 mg
SCH-MCA2494 MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applicatio 0.2 mg
SCH-MCA2494T MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applicatio 25 µg
4562-0408 MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applicati 0.2 mg
SCH-4562-0408 MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applicati 0.2 mg
X1708E Sphingosine Kinase 1 (Full Length) with N-Terminal HIS tag type: Active Enzyme host: Sf9 cells Isotype: N_A 10
X1708E Sphingosine Kinase 1 (Full Length) with N-Terminal HIS tag type: Active Enzyme host: Sf9 cells Isotype: N A 10
orb60893 Matrine((+)-Matrine) Matrine((+)-Matrine) is an alkaloid found in plants from the Sophora family. It has a variety of pharmacological effects, including anti-cancer effects, and action as a kappa opio 50 mg
18-003-44046 ES cells cDNA. RIKEN full-length enriched library. clone 2410149J18 product Kruppel-like factor 2 (lung). full insert sequence - N_A Polyclonal 0.1 mg Protein A
X2565 Influenza A, H1N1 New Caledonia 20 99, Hemagglutinin, Recombinant (Full Length) type: Recombinant Protein host: Sf9 cells 10
X2565 Influenza A, H1N1 New Caledonia 20_99, Hemagglutinin, Recombinant (Full Length) type: Recombinant Protein host: Sf9 cells 10
orb82810 Mouse MDC protein CCL22_MDC is a CC chemokine that is produced in B cells, macrophages, monocytederived dendritic cells, activated NK cells and CD4 T cells. It signals through the CCR4 receptor. MDC c 1 mg
orb82743 Human MDC protein MDC is a CC chemokine that is produced in B cells, macrophages, monocyte-derived dendritic cells, activated NK cells and CD4 T cells. It signals through the CCR4 receptor. MDC chemoa 1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur