Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :15767422
Publication Date : //

Amino acid preferences for a critical substrate binding subsite of retroviral proteases in type 1 cleavage sites.

The specificities of the proteases of 11 retroviruses representing each of the seven genera of the family Retroviridae were studied using a series of oligopeptides with amino acid substitutions in the P2 position of a naturally occurring type 1 cleavage site (Val-Ser-Gln-Asn-Tyr Pro-Ile-Val-Gln; the arrow indicates the site of cleavage) in human immunodeficiency virus type 1 (HIV-1). This position was previously found to be one of the most critical in determining the substrate specificity differences of retroviral proteases. Specificities at this position were compared for HIV-1, HIV-2, equine infectious anemia virus, avian myeloblastosis virus, Mason-Pfizer monkey virus, mouse mammary tumor virus, Moloney murine leukemia virus, human T-cell leukemia virus type 1, bovine leukemia virus, human foamy virus, and walleye dermal sarcoma virus proteases. Three types of P2 preferences were observed: a subgroup of proteases preferred small hydrophobic side chains (Ala and Cys), and another subgroup preferred large hydrophobic residues (Ile and Leu), while the protease of HIV-1 preferred an Asn residue. The specificity distinctions among the proteases correlated well with the phylogenetic tree of retroviruses prepared solely based on the protease sequences. Molecular models for all of the proteases studied were built, and they were used to interpret the results. While size complementarities appear to be the main specificity-determining features of the S2 subsite of retroviral proteases, electrostatic contributions may play a role only in the case of HIV proteases. In most cases the P2 residues of naturally occurring type 1 cleavage site sequences of the studied proteases agreed well with the observed P2 preferences.

Authors : Bagossi Péter , Sperka Tamás , Fehér Anita , Kádas János , Zahuczky Gábor , Miklóssy Gabriella , Boross Péter , Tözsér József ,

Related products :

Catalog number Product name Quantity
EIAAB46684 Homo sapiens,Human,Monocyte amino acid permease 2,MOP-2,SLC7A7,Solute carrier family 7 member 7,y(+)L-type amino acid transporter 1,Y+L amino acid transporter 1,Y+LAT1,y+LAT-1
orb81487 E.coli Dnak Substrate Binding Domain protein Recombinant DnaK Substrate Binding domain produced in E.coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecu 20
EIAAB46686 Cationic amino acid transporter, y+ system,Homo sapiens,Human,KIAA0245,SLC7A6,Solute carrier family 7 member 6,y(+)L-type amino acid transporter 2,Y+L amino acid transporter 2,Y+LAT2,y+LAT-2
29-709 BAG proteins compete with Hip for binding to the Hsc70_Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but di 0.1 mg
KP1879 Fluorescent Substrate for Glu-Specific Proteases
KP1878 Fluorescent Substrate for Asp-Specific Proteases
32-137 CK1 (also designated Cytokeratin 1 or KRT1 ),with 644-amino acid protein (about 70kDa), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specializatio 0.1 mL
orb81488 E.coli Dnak Substrate Binding Domain C-terminal protein Recombinant Dnak Substrate Binding Domain C-terminal produced in E.coli is a single, non-glycosylated polypeptide chain containing 255 amino aci 20
27-491 PCSK6 is a calcium-dependent serine endoprotease that can cleave precursor protein at their paired basic amino acid processing sites. This gene is thought to play a role in tumor progression. 0.1 mg
25-821 F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-med 0.05 mg
18-003-43732 Neutral amino acid transporter B(0) - ATB(0); Solute carrier family 1 member 5; Sodium-dependent neutral amino acid transporter type 2; RD114_simian type D retrovirus receptor; Baboon M7 virus recepto 0.1 mg Protein A
EIAAB46683 Rat,Rattus norvegicus,Slc7a7,Solute carrier family 7 member 7,y(+)L-type amino acid transporter 1,Y+L amino acid transporter 1,Y+LAT1,y+LAT-1
EIAAB46685 Mouse,Mus musculus,Slc7a7,Solute carrier family 7 member 7,y(+)L-type amino acid transporter 1,Y+L amino acid transporter 1,Y+LAT1,y+LAT-1
EIAAB46687 Kiaa0245,Mouse,Mus musculus,Slc7a6,Solute carrier family 7 member 6,y(+)L-type amino acid transporter 2,Y+L amino acid transporter 2,Y+LAT2,y+LAT-2
EIAAB36968 Amino acid transporter A1,MNat2,Mouse,Mus musculus,Nat2,N-system amino acid transporter 2,Slc38a1,Snat1,Sodium-coupled neutral amino acid transporter 1,Solute carrier family 38 member 1,System A amino
orb80793 Human EGF Receptor-Sf9 (ErbB1) Active protein EGFR Human Recombinant encoding a.a. 672-1210 expressed in Baculovirus infected Sf9 cells, fused with GST-tag at N-terminus with thrombin cleavage sites, 1
27-444 HCLS1 is a substrate for caspase cleavage during apoptosis. 0.05 mg
EIAAB25184 Aromatic amino acid transporter 1,MCT 10,Mct10,Monocarboxylate transporter 10,Rat,Rattus norvegicus,Slc16a10,Solute carrier family 16 member 10,Tat1,T-type amino acid transporter 1
EIAAB25183 Aromatic amino acid transporter 1,MCT 10,Mct10,Monocarboxylate transporter 10,Mouse,Mus musculus,Slc16a10,Solute carrier family 16 member 10,Tat1,T-type amino acid transporter 1
EIAAB36973 Amino acid transporter A2,ATA2,Homo sapiens,Human,KIAA1382,Protein 40-9-1,SAT2,SLC38A2,SNAT2,Sodium-coupled neutral amino acid transporter 2,Solute carrier family 38 member 2,System A amino acid trans
EIAAB25185 Aromatic amino acid transporter 1,Homo sapiens,Human,MCT 10,MCT10,Monocarboxylate transporter 10,SLC16A10,Solute carrier family 16 member 10,TAT1,T-type amino acid transporter 1
EIAAB37135 Amino acid transporter ATB0+,Colonic system B0+ amino acid transporter CATB0+,Mouse,Mus musculus,Slc6a14,Sodium- and chloride-dependent neutral and basic amino acid transporter B(0+),Solute carrier fa
EIAAB36958 Lyaat1,LYAAT-1,Lysosomal amino acid transporter 1,Neutral amino acid_proton symporter,Pat1,Proton_amino acid transporter 1,Proton-coupled amino acid transporter 1,Rat,Rattus norvegicus,Slc36a1,Solute
EIAAB38646 B(0,+)-type amino acid transport protein,D2,NAA-TR,Nbat,Neutral and basic amino acid transport protein rBAT,Rat,Rattus norvegicus,Slc3a1
EIAAB36967 Amino acid transporter A1,Ata1,Glnt,Glutamine transporter,N-system amino acid transporter 2,Rat,rATA1,Rattus norvegicus,Sa2,Sat1,Slc38a1,Snat1,Sodium-coupled neutral amino acid transporter 1,Solute ca

https://antibody-antibodies.com/ | https://gentaur.com/ | https://gen-script.com/ | https://diagenox.com/ | https://clonagen.com/ | http://gentaursearch.com/ | http://gentaurpub.com/ | https://gentaur-online.com/ | http://anti-anti-pdf.com/ | http://gentaur-worldwide.com/


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur

Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

spain@gentaur.com | Gentaur | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur