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[Separation of enantiomers of amino acid derivatives by high performance liquid chromatography on teicoplanin chiral stationary phase].
Direct enantioseparations of seven N-fluorenylmethoxycarbonyl-amino acids (Fmoc-Val, Fmoc-Ser, Fmoc-Leu, Fmoc-Ile, Fmoc-Met, Fmoc-Ala, and Fmoc-Phe) were achieved by high performance liquid chromatography using a 250 mm x 4. 6 mm i.d. column bonded teicoplanin on silica gel as chiral stationary phase and methanol-acetic acid-triethylamine mixtures as mobile phase. The comparison of retention behavior and enantioseparation of N-Fmoc-Val and its non-blocked analogue on a Chirobiotic column with different mobile phase systems is presented. The effects of the content of acetic acid and triethylamine and the content ratio of acetic acid and triethylamine in the mobile phase on the enantioseparation of some N-Fmoc-amino acids were examined. The results showed that variations of column temperatureand flow rate of mobile phase also had effects on enantioseparation of N-Fmoc-amino acids. The method had been successfully applied to the determination of optical purity of some derivatized amino acids.
Authors : Lin Lin , Xia Lijun , Xu Xu , Xu Hongyan ,
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WP1008: amino acid conjugation of benzoic acid
WP1127: amino acid conjugation of benzoic acid
WP1252: amino acid conjugation of benzoic acid
WP1287: amino acid conjugation of benzoic acid
WP1577: amino acid conjugation of benzoic acid
WP1621: Arginine and proline metabolism
WP1847: Metabolism of amino acids and derivatives
WP521: amino acid conjugation of benzoic acid
WP662: Amino Acid metabolism
WP7: Sulfur Amino Acid biosynthesis
WP715: amino acid conjugation
WP891: amino acid conjugation of benzoic acid
WP1020: Fatty Acid Biosynthesis
WP1021: Phase I, non P450
WP105: Fatty Acid Beta Oxidation 2
WP1061: Fatty Acid Beta Oxidation
WP1075: Folic Acid Network
WP1107: Mitochondrial LC-Fatty Acid Beta-Oxidation
WP1139: Fatty Acid Biosynthesis
WP1140: Phase I, non P450
WP1177: Fatty Acid Beta Oxidation
WP1192: Folic Acid Network
WP1207: Fatty Acid Beta Oxidation
WP121: Colanic Acid Building Blocks Biosynthesis
WP1213: Phase I, non P450
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