Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :16846028
Publication Date : //

Derivation and characterization of alveolar epithelial cells from murine embryonic stem cells in vitro.


We present a protocol that has been developed for induction of the differentiation of murine embryonic stem (ES) cells to alveolar type II cells. With this protocol, undifferentiated murine ES cells are induced to form embryoid bodies (EBs). The 10-d-old EBs are transferred to adherent culture conditions and are fed with high-glucose Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% v/v fetal bovine serum, 2 mM L-glutamine, and 0.1 mM 2-mercaptoethanol for 20 d without splitting. Then, the cells are fed with a medium designed for the maintenance and growth of mature distal airway epithelial cells (small airway growth medium, SAGM) for 3 d. Characterization of the alveolar type II cells was done using real-time reverse transcriptase polymerase chain reaction detection of surfactant protein C mRNA and immunocytochemical detection of prosurfactant protein C. Real-time reverse transcriptase polymerase chain reaction revealed that SAGM increases the mRNA expression level of SPC by a factor of 8 when compared to that of cells grown in supplemented high-glucose DMEM (p < 0.05, Student t-test). Immunocytochemistry revealed that proSPC-expressing cells comprised 2.8 +/- 0.23% of the total cell population in SAGM-treated samples and 0.5 +/- 0.1% in samples treated with supplemented high-glucose DMEM (p < 0.05, chi2 test).

Authors : Samadikuchaksaraei Ali , Bishop Anne E ,

Related products :

Catalog number Product name Quantity
MUBES-01001 C57BL_6 Mouse Embryonic Stem Cells Cells Differentiation_Stem Cells 1 x 10^6 cells/vial
MUBES-01101 C57BL_6 Mouse Embryonic Stem Cells with GFP Cells Differentiation_Stem Cells 1 x 10^6 cells/vial
MUBES-01201 C57BL_6 Mouse Embryonic Stem Cells with RFP Cells Differentiation_Stem Cells 1 x 10^6 cells/vial
MUAES-01001 129 Mouse Embryonic Stem Cells Cells Differentiation_Stem Cells 1 x 10^6 cells/vial
MUAES-01201 129 Mouse Embryonic Stem Cells with RFP Cells Differentiation_Stem Cells 1 x 10^6 cells/vial
MUAES-01101 129 Mouse Embryonic Stem Cells with GFP Cells Differentiation_Stem Cells 1 x 10^6 cells/vial
MUBES-01001 C57BL_6 Mouse Embryonic Stem Cells Cells 1 x 10^6 cells/vial
MUBES-01201 C57BL_6 Mouse Embryonic Stem Cells with RFP Cells 1 x 10^6 cells/vial
MUBES-01101 C57BL_6 Mouse Embryonic Stem Cells with GFP Cells 1 x 10^6 cells/vial
MUAES-01201 129 Mouse Embryonic Stem Cells with RFP Cells 1 x 10^6 cells/vial
MUAES-01101 129 Mouse Embryonic Stem Cells with GFP Cells 1 x 10^6 cells/vial
MUAES-01001 129 Mouse Embryonic Stem Cells Cells 1 x 10^6 cells/vial
SL100489-EPI Transfection Reagents and Kits, GenJet™ DNA In Vitro Tranfection Reagent for epithelial was pre_optimized for most efficiently transfecting epithelial cells. 1 ml
9-80323 Rat Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture 1 x 100 ml
9-25096 Rat Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture 5 x 100 ml
7-15293 Rat Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture Kit
2-82596 Rat Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture Kit
9-26096 Rat Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture set
4-28323 Rat Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture Kit
4-28315 Human Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture Kit
9-32101 Mouse Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture 5 x 100 ml
2-96122 Human Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture Kit
9-80307 Mouse Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture 1 x 100 ml
7-15277 Mouse Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture Kit
9-33101 Mouse Lung PrimaCell™ 7: Alveolar Epithelial Cells II , primary cell culture set


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur