Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :17658766
Publication Date : //

Automated MALDI matrix deposition method with inkjet printing for imaging mass spectrometry.


Careful matrix deposition on tissue samples for matrix-assisted laser desorption/ionization (MALDI) is critical for producing reproducible analyte ion signals. Traditional methods for matrix deposition are often considered an art rather than a science, with significant sample-to-sample variability. Here we report an automated method for matrix deposition, employing a desktop inkjet printer (<$200) with 5760 x 1440 dpi resolution and a six-channel piezoelectric head that delivers 3 pL/drop. The inkjet printer tray, designed to hold CDs and DVDs, was modified to hold microscope slides. Empty ink cartridges were filled with MALDI matrix solutions, including DHB in methanol/water (70:30) at concentrations up to 40 mg/mL. Various samples (including rat brain tissue sections and standards of small drug molecules) were prepared using three deposition methods (electrospray, airbrush, inkjet). A linear ion trap equipped with an intermediate-pressure MALDI source was used for analyses. Optical microscopic examination showed that matrix crystals were formed evenly across the sample. There was minimal background signal after storing the matrix in the cartridges over a 6-month period. Overall, the mass spectral images gathered from inkjet-printed tissue specimens were of better quality and more reproducible than from specimens prepared by the electrospray and airbrush methods.

Authors : Baluya Dodge L , Garrett Timothy J , Yost Richard A ,

Related products :

Catalog number Product name Quantity
S-0030.1 Protein Sequencing by Mass Spectrometry - Complex
S-0030 Protein Sequencing by Mass Spectrometry - Standard
S-0032 Post Translational Modification Mapping (PTM) by Mass Spectrometry
S-0030.2 Quantitative Mass Spectrometry using the Label free Quantitation protocol.
22110010-2 MALDI TOF mass spec analysis 1
22110010-1 MALDI TOF mass spec analysis 1
p9106-1gm MBT MALDI-MS Matrix CAS: 149-30-4 1 gram
p9101-1g DHB MALDI-MS Matrix CAS: 490-79-9 1 gram
p9102-1g SA MALDI-MS Matrix CAS: 530-59-6 1 gram
p9105-1g DHAP MALDI-MS Matrix CAS: 89-84-9 1 gram
p9106-4x25mg MBT MALDI-MS Matrix CAS: 149-30-4 4 x 25 mg
p9102-25mg SA MALDI-MS Matrix CAS: 530-59-6 25 mg
p9106-25mg MBT MALDI-MS Matrix CAS: 149-30-4 25 mg
p9104-1g THAP MALDI-MS Matrix CAS: 480-66-0 1 gram
p9102-4x25mg SA MALDI-MS Matrix CAS: 530-59-6 4 x 25 mg
p9101-4x25mg DHB MALDI-MS Matrix CAS: 490-79-9 4 x 25 mg
p9101-25mg DHB MALDI-MS Matrix CAS: 490-79-9 25 mg
p9103-1g HABA MALDI-MS Matrix CAS: 1634-82-8 1 gram
p9105-4x25mg DHAP MALDI-MS Matrix CAS: 89-84-9 4 x 25 mg
p9104-25mg THAP MALDI-MS Matrix CAS: 480-66-0 25 mg
p9105-25mg DHAP MALDI-MS Matrix CAS: 89-84-9 25 mg
p9104-4x25mg THAP MALDI-MS Matrix CAS: 480-66-0 4 x 25 mg
p9100-1g CHCA MALDI-MS Matrix CAS: 28166-41-8 1 gram
p9100-4x25mg CHCA MALDI-MS Matrix CAS: 28166-41-8 4 x 25 mg
p9103-25mg HABA MALDI-MS Matrix CAS: 1634-82-8 25 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur