Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :19953299
Publication Date : //

Pistil drip following pollination: a simple in planta Agrobacterium-mediated transformation in cotton.


Transgenic cotton plants were developed by pistil drip inoculation in a solution containing Agrobacterium carrying a gene for resistance to the herbicide Basta (bar), 10% (w/v) sucrose, 0.05% (v/v) Silwet L-77 and 40 mg acetosyringone l(-1). Pistil drip during 17:00-19:00 on the first day of flowering resulted in 0.07-0.17% Basta-resistant plants/number of viable seeds generated, and stigma excision prior to pistil drip during this time period gave rise to a transformation efficiency of 0.46-0.93%, in contrast with 0.04-0.06% generated from pistil drip during 9:00-11:00 on the second day of flowering. PCR and Southern blot analysis confirmed the integration of the bar gene into the cotton genome, and a T1 and T2 generation herbicide resistance test consistently revealed expression and stable heritability of the bar gene in the two generations.

Authors : Tianzi Chen , Shenjie Wu , Jun Zhao , Wangzhen Guo , Tianzhen Zhang ,

Related products :

Catalog number Product name Quantity
28-359 ZNF271 may be involved in transcriptional regulation. It plays a role in the EBV-mediated transformation process 0.05 mg
28-360 ZNF271 may be involved in transcriptional regulation. It plays a role in the EBV-mediated transformation processZinc finger proteins interact with nucleic acids and have diverse functions. The zinc fi 0.1 mg
30-667 LIN9 belongs to the lin-9 family and acts as a tumor suppressor. It inhibits DNA synthesis. Its ability to inhibit oncogenic transformation is mediated through its association with RB1. LIN9 plays a r 0.05 mg
30-666 LIN9 belongs to the lin-9 family and acts as a tumor suppressor. It inhibits DNA synthesis. Its ability to inhibit oncogenic transformation is mediated through its association with RB1. LIN9 plays a r 0.05 mg
CT331-100 100G Cotton Degreese Cotton & Adhesive Plaster 1x5
CT331-100 100G Cotton Degreese Cotton & Adhesive Plaster 1
CT331-500 500G Cotton Degreese Cotton & Adhesive Plaster 1
CT331-500 500G Cotton Degreese Cotton & Adhesive Plaster 1
CT331-100 100G Cotton Degreese Cotton & Adhesive Plaster 1
CT331-50 50G Cotton Degreese Cotton & Adhesive Plaster 1x10
CT331-50 50G Cotton Degreese Cotton & Adhesive Plaster 1
CT331-50 50G Cotton Degreese Cotton & Adhesive Plaster 1
BC001 Calcium Chloride E.coli Transformation Buffer, E.coli Transformation 5x250ml
29-961 Transport of folate compounds into mammalian cells can occur via receptor-mediated or carrier-mediated mechanisms. A functional coordination between these 2 mechanisms has been proposed to be the meth 0.1 mg
GRC-180 Cotton Rat Genomic DNA Species: Cotton Rat Control Genomic DNA 0.1mg
30627031-1 YEB Agrobacterium Growth Medium 500 g
953914-0006 DRIP TIP PTFE Case Qty: 1 x 10
CSB-RP039474Ba Recombinant Agrobacterium tumefaciens T-DNA border endonuclease virD2 500ug
CSB-RP039444Ba Recombinant Agrobacterium tumefaciens T-DNA border endonuclease virD2 500ug
CSB-RP039494Ba Recombinant Agrobacterium tumefaciens T-DNA border endonuclease virD2 500ug
ACCFL0017 FLOW CELL DRIP CHAMBER x 2
ACCFL0017 FLOW CELL DRIP CHAMBER
18140-400 CONDENSER,LIEBIG,DRIP,400MM Case Qty:1
18140-400 CONDENSER,LIEBIG,DRIP,400MM 1
PE039444Ba Recombinant Agrobacterium tumefaciens T-DNA border endonuclease virD2 protein 200ug


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur