Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :20162536
Publication Date : //

Reagents for investigating MAPK signalling in model yeast species.


Here we present a set of resources (bacterial expression plasmids and antibodies) for the interrogation of proteins involved in yeast MAPK signalling. We constructed bacterial protein expression plasmids for 25 proteins involved in MAPK signalling in budding yeast. From these constructs we expressed and purified proteins and generated rabbit polyclonal antibodies against 13 proteins in the pheromone MAPK pathway. We verified the specificity of the antibodies and employed them to follow pathway proteins in cells stimulated with pheromone. We show that these reagents can be used to detect pheromone-induced post-translational modifications and changes in the oligomeric state of pathway proteins. In addition to recognizing their target proteins in Saccharomyces cerevisiae, these antibodies allow the detection of predicted orthologues in the distant evolutionary relatives Kluyveromyces lactis and Schizosaccharomyces pombe. These antibodies are new tools for investigating MAPK signalling in model yeast species and may be useful for studying MAPK signalling in higher eukaryotes.

Authors : Pincus David , Benjamin Kirsten , Burbulis Ian , Tsong Annie E , Resnekov Orna ,

Related products :

Catalog number Product name Quantity
B366 *Yeast Nitrogen Base w_o Amino Acids USE For investigating carbon and nitrogen requirements of yeasts. Qty per Litre of Medium: 6,7
B366 Yeast Nitrogen Base w_o Amino Acids USE: For investigating carbon and nitrogen requirements of yeasts. 5x500gm
SCH-AHP905 RABBIT ANTI p38 MAPK (pThr180_Tyr182), Product Type Polyclonal Antibody, Specificity p38 MAPK , Target Species Rat, Host Rabbit, Format Purified, Isotypes Polyclonal IgG, Applications WB*, Clon 0.1 ml
AHP905 RABBIT ANTI p38 MAPK (pThr180_Tyr182), Product Type Polyclonal Antibody, Specificity p38 MAPK , Target Species Rat, Host Rabbit, Format Purified, Isotypes Polyclonal IgG, Applications WB*, Clon 0.1 ml
EST24-P Yeast Telomerase_EST2 Control_blocking peptide _ 4 Species Reactivity: yeast 100 ug Product tipe: Peptide
EST24-S Anti-Yeast Telomerase_EST2 antiserum _ 4 Species Reactivity: yeast 100 ul Product tipe: Antiserum
EST25-P Yeast Telomerase_EST2 Control_blocking peptide _ 5 Species Reactivity: yeast 100 ug Product tipe: Peptide
EST24-A Anti-Yeast Telomerase_EST2 IgG _ 4, aff pure Species Reactivity: yeast 100 ug Product tipe: Antibodies
EST25-S Anti-Yeast Telomerase_EST2 antiserum _ 5 Species Reactivity: yeast 100 ul Product tipe: Antiserum
SPR-314C Recombinant Yeast Aha1 Protein Species: Yeast
SPR-314B Recombinant Yeast Aha1 Protein Species: Yeast
SPR-314A Recombinant Yeast Aha1 Protein Species: Yeast
ANTY021245 Polyclonal Antibodies: p38 MAPK(Ab-182) ; Specificity: p38 MAPK(Ab-182) ; Application: IHC 100ug
E1206m ELISA kit Erk2,ERK-2,ERT1,Extracellular signal-regulated kinase 2,MAP kinase 1,MAP kinase 2,MAP kinase isoform p42,Mapk,MAPK 1,MAPK 2,Mapk1,Mitogen-activated protein kinase 1,Mitogen-activated protei 96T
E1206r ELISA kit Erk2,ERK-2,ERT1,Extracellular signal-regulated kinase 2,MAP kinase 1,MAP kinase 2,MAP kinase isoform p42,Mapk,MAPK 1,MAPK 2,Mapk1,Mitogen-activated protein kinase 1,Mitogen-activated protei 96T
0619-9010 SYNTHETIC YEAST ANTIPAIN, Product Type Purified Protein, Specificity ANTIPAIN, Target Species Yeast, Host N_A, Format Purified, Isotypes , Applications E, Clone 10 mg
SCH-0619-9010 SYNTHETIC YEAST ANTIPAIN, Product Type Purified Protein, Specificity ANTIPAIN, Target Species Yeast, Host N_A, Format Purified, Isotypes , Applications E, Clone 10 mg
U1206m CLIA Erk2,ERK-2,ERT1,Extracellular signal-regulated kinase 2,MAP kinase 1,MAP kinase 2,MAP kinase isoform p42,Mapk,MAPK 1,MAPK 2,Mapk1,Mitogen-activated protein kinase 1,Mitogen-activated protein kina 96T
U1206r CLIA Erk2,ERK-2,ERT1,Extracellular signal-regulated kinase 2,MAP kinase 1,MAP kinase 2,MAP kinase isoform p42,Mapk,MAPK 1,MAPK 2,Mapk1,Mitogen-activated protein kinase 1,Mitogen-activated protein kina 96T
E1206m ELISA Erk2,ERK-2,ERT1,Extracellular signal-regulated kinase 2,MAP kinase 1,MAP kinase 2,MAP kinase isoform p42,Mapk,MAPK 1,MAPK 2,Mapk1,Mitogen-activated protein kinase 1,Mitogen-activated protein kin 96T
E1206r ELISA Erk2,ERK-2,ERT1,Extracellular signal-regulated kinase 2,MAP kinase 1,MAP kinase 2,MAP kinase isoform p42,Mapk,MAPK 1,MAPK 2,Mapk1,Mitogen-activated protein kinase 1,Mitogen-activated protein kin 96T
AA0033 MAPK Phospho-Specific Array includes 185 highly specific and well-characterized phosphorylation antibodies in the MAPK pathway. 2 Pieces/Box
4670-1202 NATIVE YEAST GLUCOSE_6_PHOSPHATE DEHYDROGENASE, Product Type Purified Protein, Specificity GLUCOSE_6_PHOSPHATE DEHYDROGENASE, Target Species Yeast, Host N_A, Format Purified, Isotypes , Applicat 1 KU
SCH-4670-1202 NATIVE YEAST GLUCOSE_6_PHOSPHATE DEHYDROGENASE, Product Type Purified Protein, Specificity GLUCOSE_6_PHOSPHATE DEHYDROGENASE, Target Species Yeast, Host N_A, Format Purified, Isotypes , Applicat 1 KU
SCH-AHP1294 RABBIT ANTI YEAST RAD9, Product Type Polyclonal Antibody, Specificity RAD9, Target Species Yeast, Host Rabbit, Format Purified, Isotypes Polyclonal IgG, Applications E, WB, Clone 50 µg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur