Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :20417643
Publication Date : //

Mechanism of U-insertion RNA editing in trypanosome mitochondria: characterization of RET2 functional domains by mutational analysis.


3'-Terminal uridylyl transferases (TUTases) selectively bind uridine 5'-triphosphate (UTP) and catalyze the addition of uridine 5'-monophosphate to the 3'-hydroxyl of RNA substrates in a template-independent manner. RNA editing TUTase 1 and RNA editing TUTase 2 (RET2) play central roles in uridine insertion/deletion RNA editing, which is an essential part of mitochondrial RNA processing in trypanosomes. Although the conserved N-terminal (catalytic) domain and C-terminal (nucleotide base recognition) domain are readily distinguished in all known TUTases, nucleotide specificity, RNA substrate preference, processivity, quaternary structures, and auxiliary domains vary significantly among enzymes of divergent biological functions. RET2 acts as a subunit of the RNA editing core complex to carry out guide-RNA-dependent U-insertion into mitochondrial mRNA. By correlating mutational effects on RET2 activity as recombinant protein and as RNA editing core complex subunit with RNAi-based knock-in phenotypes, we have assessed the UTP and RNA binding sites in RET2. Here we demonstrate functional conservation of key UTP-binding and metal-ion-coordinating residues and identify amino acids involved in RNA substrate recognition. Invariant arginine residues 144 and 435 positioned in the vicinity of the UTP binding site are critical for RET2 activity on single-stranded and double-stranded RNAs, as well as function in vivo. Recognition of a double-stranded RNA, which resembles a guide RNA/mRNA duplex, is further facilitated by multipoint contacts across the RET2-specific middle domain.

Authors : Ringpis Gene-Errol , Stagno Jason , Aphasizhev Ruslan ,

Related products :

Catalog number Product name Quantity
XGC-BiRDS-MitoSeq-Denovo Bioinformatics of data for comprehensive mitochondria genome analysis Sample
40267 Ret2 10 µg
40267 Ret2, Active Human Recombinant Protein 10 µg
ABP-FP-WMIT Alleleustrious pWasabi-Mitochondria fusion vector Mitochondria 5 ug
ABP-FP-TMIT Alleleustrious pmTFP1-Mitochondria fusion vector Mitochondria 5 ug
EIAAB34197 Adarb1,Double-stranded RNA-specific editase 1,dsRNA adenosine deaminase,Rat,Rattus norvegicus,Red1,RNA-editing deaminase 1,RNA-editing enzyme 1
EIAAB34196 Adar2,Adarb1,Double-stranded RNA-specific editase 1,dsRNA adenosine deaminase,Mouse,Mus musculus,Red1,RNA-editing deaminase 1,RNA-editing enzyme 1
EIAAB34195 ADAR2,ADARB1,Double-stranded RNA-specific editase 1,DRADA2,dsRNA adenosine deaminase,Homo sapiens,Human,RED1,RNA-editing deaminase 1,RNA-editing enzyme 1
28-247 CLCNKA is a member of the CLC family of voltage-gated chloride channels. It is predicted to have 12 transmembrane domains, and requires a beta subunit called barttin to form a functional channel. It i 0.05 mg
M1722 Tetramethylrhodamine, ethyl ester (TMRE), Positively charged lipophilic red-orange fluorophore; rapidly accumulates in mitochondria due to relative negative charge of active mitochondria with respect
31-187 Tel encodes an ETS family transcription factor. The product of this gene contains two functional domains a N-terminal pointed (PNT) domain that is involved in the protein-protein interactions with it 0.1 mg
31-186 ETV6 (ETS-related protein Tel1, ETS translocation variant 6 , Tel) is an ETS family transcription factor. This protein contains two functional domains a N-terminal pointed (PNT) domain that is involv 0.05 mg
26-189 ABI3BP contains 2 fibronectin type-III domains. The loss of ABI3BP expression could play a functional role in thyroid tumorigenesis. It also presumably represents a trigger gene for evoking cellular s 0.05 mg
EIAAB34198 Adar3,Adarb2,Double-stranded RNA-specific editase B2,dsRNA adenosine deaminase B2,Mouse,Mus musculus,Red2,RNA-dependent adenosine deaminase 3,RNA-editing deaminase 2,RNA-editing enzyme 2
EIAAB34199 Adar3,Adarb2,Double-stranded RNA-specific editase B2,dsRNA adenosine deaminase B2,Rat,Rattus norvegicus,Red2,RNA-dependent adenosine deaminase 3,RNA-editing deaminase 2,RNA-editing enzyme 2
EIAAB34200 ADAR3,ADARB2,Double-stranded RNA-specific editase B2,dsRNA adenosine deaminase B2,Homo sapiens,Human,RED2,RNA-dependent adenosine deaminase 3,RNA-editing deaminase 2,RNA-editing enzyme 2
25-255 KCNK15 is one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. KCNK15 has not been shown to be a functional channel, however, it may require other 0.05 mg
25-654 ADARB2 is a member of the double-stranded RNA adenosine deaminase family of RNA-editing enzymes and may play a regulatory role in RNA editing. This gene encodes a member of the double-stranded RNA ade 0.05 mg
TotalLabQuant Base package*: 1D image analysis plus colony counting and array analysis
AS-MRA-P8 MARIA™ ANALYSIS SERVICE 8-plex analysis Environmental Testing Services 1 sample
XGC-BiRDS-MeDIP-Seq Bioinformatics analysis of Methylome data for Methylation analysis Sample
AS-MRA-P5 MARIA™ ANALYSIS SERVICE 5-plex analysis Environmental Testing Services 1 sample
XGC-BiRDS-WGBS Bioinformatics analysis of Whole Genome Bisulphite Sequencing data for Methylation analysis Sample
TotalLab Quant Base package* 1D image analysis plus colony counting and array analysis 1
TotalLab Quant Base package* 1D image analysis plus colony counting and array analysis


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur