Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :21660946
Publication Date : //

Adipogenic differentiation of human mesenchymal stromal cells is down-regulated by microRNA-369-5p and up-regulated by microRNA-371.


Long-term culture of human mesenchymal stromal cells (MSC) has implications on their proliferation and differentiation potential and we have demonstrated that this is associated with up-regulation of the five microRNAs miR-29c, miR-369-5p, miR-371, miR-499, and let-7f. In this study, we examined the role of these senescence-associated microRNAs for cellular aging and differentiation of MSC. Proliferation was reduced upon transfection with miR-369-5p, miR-371, and miR-499. Adipogenic differentiation was impaired by miR-369-5p whereas it was highly increased by miR-371. This was accompanied by respective gene expression changes of some adipogenic key molecules (adiponectin and fatty acid-binding protein 4 [FABP4]). Furthermore luciferase reporter assay indicated that FABP4 is a direct target of miR-369-5p. Microarray analysis upon adipogenic or osteogenic differentiation revealed down-regulation of several microRNAs albeit miR-369-5p and miR-371 were not affected. Expression of the de novo DNA methyltransferases DNMT3A and DNMT3B was up-regulated by transfection of miR-371 whereas expression of DNMT3A was down-regulated by miR-369-5p. In summary, we identified miR-369-5p and miR-371 as antagonistic up-stream regulators of adipogenic differentiation and this might be indirectly mediated by epigenetic modifications.

Authors : Bork Simone , Horn Patrick , Castoldi Mirco , Hellwig Isabelle , Ho Anthony D , Wagner Wolfgang ,

Related products :

Catalog number Product name Quantity
GUXMX-90031 Mesenchymal Stem Cell Adipogenic Differentiation Medium Cell Culture Media Cell Screening, Differentiation_Stem Cells 400 mL
5010-024-K Mesenchymal Stem Cell Adipogenic Differentiation Kit 24 wells
GUXMX-90031 Mesenchymal Stem Cell Adipogenic Differentiation Medium 400 mL
GUXMX-90031 Mesenchymal Stem Cell Adipogenic Differentiation Medium Cell Culture Media 400 mL
PMIRH1202PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1202 Bacterial Streak
PMIRH1200PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1200 Bacterial Streak
PMIRH1203PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1203 Bacterial Streak
PMIRH1197PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1197 Bacterial Streak
PMIRH1185-2PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1185-2 Bacterial Streak
PMIRH1204PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1204 Bacterial Streak
PMIRH1205PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1205 Bacterial Streak
PMIRH369PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-369+412 MI0002464 Bacterial Streak
PMIRH1185-1PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1185-1 Bacterial Streak
PMIRH1184PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1184 Bacterial Streak
PMIRH101-1PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-101-1 MI0000103 Bacterial Streak
PMIRH320d-2PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-320d-2 Bacterial Streak
PMIRH106aPA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-106a Bacterial Streak
PMIRH1179PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1179 Bacterial Streak
PMIRH1180PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1180 Bacterial Streak
PMIRH1181PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1181 Bacterial Streak
PMIRH1182PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1182 Bacterial Streak
PMIRH1183PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-1183 Bacterial Streak
PMIRH548i-3PA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-548i-3 Bacterial Streak
PMIRH892bPA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-892b Bacterial Streak
PMIRH892aPA-1 MicroRNA Tools Human pre-microRNA Expression Construct Lenti-miR-892a Bacterial Streak


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur