Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :22198332
Publication Date : //

Functional analysis of two lebocin-related proteins from Manduca sexta.


Insects produce a group of antimicrobial peptides (AMPs) in response to microbial infections. Most AMPs are synthesized as inactive precursors/pro-proteins and require proteolytic processing to generate small active peptides. Here we report identification and functional analysis of two lebocin-related proteins (Leb-B and Leb-C) from the tobacco hornworm, Manduca sexta. The mRNA levels of Leb-B and Leb-C increased significantly in larval fat body and hemocytes after injection of Escherichia coli, Micrococcus luteus and Saccharomyces cerevisiae. Western blotting using rabbit polyclonal antibody to Leb-B showed accumulation of large protein(s) and small peptide(s) in larval hemolymph after microbial injection. This result and the presence of RXXR motifs in the deduced amino acid sequences led to our postulation that Leb-B/C may be inactive precursors that are processed in larval hemolymph to generate short active peptides. To test this hypothesis, we expressed and purified full-length and various fragments of Leb-B and Leb-C as thioredoxin (TRX) fusion proteins. We found that fusion proteins could be cleaved by induced larval plasma, and the cleavage sites were determined by protein sequencing. Antibacterial activity of peptide fragments was also verified using synthetic peptides, and active M. sexta lebocin peptides were located at the N-termini of Leb-B/C, which are different from Bombyx mori lebocins 1-4 that are located close to the C-termini. In addition, we found that synthetic Leb-B(22-48) peptide not only had higher antibacterial activity but also caused agglutination of E. coli cells. Our results provide valuable information for studying processing of lebocin precursors in lepidopteran insects.

Authors : Rao Xiang-Jun , Xu Xiao-Xia , Yu Xiao-Qiang ,

Related products :

Catalog number Product name Quantity
H-5792.0001 Adipokinetic Hormone (Apis mellifera ligustica, Bombyx mori, Heliothis zea, Manduca sexta) Salt _ Binding _ Synonym AKH (Apis mellifera ligustica, Bombyx mori, Heliothis zea, Manduca sexta) SumForm 1.0 mg
H-5792.0001 Adipokinetic Hormone (Apis mellifera ligustica, Bombyx mori, Heliothis zea, Manduca sexta) Salt _ Binding _ Synonym AKH (Apis mellifera ligustica, Bombyx mori, Heliothis zea, Manduca sexta) SumForm 1.0 mg
A-0601 Adipokinetic Hormone (Apis mellifera ligustica, Bombyx mori, Heliothis zea, Manduca sexta) AKH (Apis mellifera ligustica, Bombyx mori, Heliothis zea, Manduca sexta) 98 percent C47H65N11O14 CAS: 99 5mg
orb71551 Ecdysis-Triggering Hormone (Manduca sexta) ; Mas-ETH peptide This is Ecdysis-Triggering Hormone (Manduca sexta) ; Mas-ETH peptide. For research use only. 1 mg
orb70403 Apis mellifera ligustica, Bombyx mori, Heliothis zea, Manduca sexta Adipokinetic Hormone peptide This is Apis mellifera ligustica, Bombyx mori, Heliothis zea, Manduca sexta Adipokinetic Hormone peptid 1 mg
047-28 Allatotropin (AT)(Manduca sexta) _ 500ug 500 µg
047-57 Allatotropin (6_13)(Manduca sexta) _ 500ug 500 µg
070-24 Diuretic Hormone (DH)(Manduca sexta) _ 200ug 200 µg
047-06 Adipokinetic Hormone (Heliothis zea and Manduca sexta) _ 1mg 1 mg
SP3524a Bombyx mori, Heliothis zea, Manduca sexta) 1mg
FC3-070-24 Diuretin Hormone (DH)(Manduca sexta) _ Cy3 Labeled _ 1 nmol 1 nmol
FG-070-24A Diuretin Hormone (DH)(Manduca sexta) _ FAM Labeled _ 1 nmol 1 nmol
SP3524b Bombyx mori, Heliothis zea, Manduca sexta) 5mg
SP-102038-1 Ecdysis_Triggering Hormone (Manduca sexta) ; Mas_ETH (AA Ser_Asn_Glu_Ala_Ile_Ser_Pro_Phe_Asp_Gln_Gly_Met_Met_Gly_Tyr_Val_Ile_Lys_Thr_Asn_Lys_Asn_Ile_Pro_Arg_Met_NH2) (MW 2941.45) 1 mg
B-070-24 Diuretin Hormone (DH)(Manduca sexta) _ Biotin Labeled _ 20ug 20 µg
Y090053 Anti_ecdysone receptor (EcR) (common), Manduca sexta Monoclonal 100ul
FR-070-24 Diuretin Hormone (DH)(Manduca sexta) _ Rhodamine Labeled _ 1 nmol 1 nmol
SP-102038-1 Ecdysis-Triggering Hormone (Manduca sexta) ; Mas-ETH (AA: Ser-Asn-Glu-Ala-Ile-Ser-Pro-Phe-Asp-Gln-Gly-Met-Met-Gly-Tyr-Val-Ile-Lys-Thr-Asn-Lys-Asn-Ile-Pro-Arg-Met-NH2) (MW: 2941.45) 1 mg
Y090080 Anti_ecdysone receptor (EcR) (common), Manduca sexta Monoclonal 100ul
T-070-24 Diuretin Hormone (DH)(Manduca sexta) _ Iodine 125 Labeled Tracer _ 10uCi 10 µCi
102038 Ecdysis_Triggering Hormone (Manduca sexta) ; Mas_ETH Formula C127H206N36O38S3 Sequence Ser_Asn_Glu_Ala_Ile_Ser_Pro_Phe_Asp_Gln_Gly_Met_Met_Gly_Tyr_Val_Ile_Lys_Thr_Asn_Lys_Asn_Ile_Pro_Arg_Met_NH2 1mg
Y090080 Anti-ecdysone receptor (EcR) (common), Manduca sexta Monoclonal Antibody 100ul
Y090053 Anti-ecdysone receptor (EcR) (common), Manduca sexta Monoclonal Antibody 100ul
SP-102038-1 Ecdysis-Triggering Hormone (Manduca sexta) ; Mas-ETH (AA: Ser-Asn-Glu-Ala-Ile-Ser-Pro-Phe-Asp-Gln-Gly-Met-Met-Gly-Tyr-Val-Ile-Lys-Thr-Asn-Lys-Asn-Ile-Pro-Arg-Met-NH2) (MW: 2941.45) Species Reactivity: 1 mg Product tipe: Pure Peptide
SP-100043-5 Adipokinetic Hormone (Apis mellifera ligustica, Bombyx mori, Heliothis zea, Manduca sexta) (AA: Glp-Leu-Thr-Phe-Thr-Ser-Ser-Trp-Gly-NH2) (MW: 921) 5 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur