Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :23513455
Publication Date : //

Development of indirect competitive fluorescence immunoassay for 2,2',4,4'-tetrabromodiphenyl ether using DNA/dye conjugate as antibody multiple labels.


An indirect competitive fluorescence immunoassay using a DNA/dye conjugate as antibody multiple labels was developed on 96-well plates for the identification and quantification of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) in aqueous samples. A hapten, 2,4,2'-tribromodiphenyl ether-4'-aldehyde, was synthesized, and was conjugated to bovine serum albumin to form a coating antigen. Specific recognition of the antigen by anti-PBDE antiserum was confirmed by a surface plasmon resonance measurement. In the immunoassay, the coating antigen was adsorbed on a 96-well plate first, and a sample, antiserum and biotinylated goat anti-rabbit secondary antibody were then added and reacted sequentially. A biotinylated, double-stranded DNA with 219 base pairs was attached to the secondary antibody by using streptavidin as a molecular bridge. In situ multiple labeling of the antibody was accomplished after addition of a DNA-binding fluorescent dye, SYBR Green I. The working range of the immunoassay for the BDE-47 standard was 3.1-390 microg/L, with an IC50 value of 15.6 microg/L. The calculated LOD of the immunoassay is 0.73 microg/L. The immunoassay demonstrated relatively high selectivity for BDE-47, showing very low cross-reactivity (< 3%) with BDE-15, BDE-153 and BDE-209. With a spiked river water sample containing 50 microg/L BDE-47, quantification by the immunoassay was 41.9 microg/L, which compared well with the standard GC-ECD method (45.7 microg/L). The developed immunoassay provides a rapid screening tool for polybrominated diphenyl ethers in environmental samples.

Authors : Fan Zi-Yan , Keum Young Soo , Li Qing-Xiao , Shelver Weilin L , Guo Liang-Hong ,

Related products :

Catalog number Product name Quantity
40088-47-9 Tetrabromodiphenyl ether diphenyl ether, tetrabro 1g
HRP9200 Creatinine(N)-HRP, Specificity: Each conjugate comprises antigen covalently bound to horseradish peroxide and is suitable as a tracer in immunoassay development. 0.5ml
HRP9608 Warfarin-HRP, Specificity: Each conjugate comprises antigen covalently bound to horseradish peroxide and is suitable as a tracer in immunoassay development. 0.5ml
HRP9371 Cyclic AMP-HRP, Specificity: Each conjugate comprises antigen covalently bound to horseradish peroxide and is suitable as a tracer in immunoassay development. 0.5ml
HRP9477 Creatinine-HRP, Specificity: Each conjugate comprises antigen covalently bound to horseradish peroxide and is suitable as a tracer in immunoassay development. 0.5ml
CUST-PROD Custom Immunoassay Development Program Assay Development
27-805 ODAG (Ocular development-associated gene), a novel transcription factor located on chromosome 7, encodes a protein that may play a role in eye development. mRNA profiling in multiple human tissue indi 0.05 mg
M0236 D_Luciferin, 6'_methyl ether, Competitive Inhibitor Of Firefly Luciferase, 5mg
M0236 D-Luciferin, 6'-methyl ether, Competitive Inhibitor Of Firefly Luciferase, 5mg
K40000 Surf\'s Up Surfactant Kit Surfactants for immunoassay development 1 kit
K40000 Surf's Up Surfactant Kit Surfactants for immunoassay development other 1 kit
29-175 TFAP2E belongs to AP-2 family of transcription factors which play an important role in regulating gene expression during development and differentiation of multiple organs and tissues. It may play an 0.1 mg
30-332 DISC1 is a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its inter 0.05 mg
629-64-1 n-Heptyl ether = Diheptyl ether = n-Hepta Enanthic ether = Di-n-h 1g
1579-40-4 Di_4_Tolyl Ether = Di_p_Tolyl Ether= Bis(4 Tolyl Ether 1g
11541 Amplite Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence* 1 kit
11540 Amplite™ Fluorimetric Goat Anti_Mouse IgG_HRP Conjugate ELISA Assay Kit *Red Fluorescence* 1 kit
11540 Amplite™ Fluorimetric Goat Anti_Mouse IgG_HRP Conjugate ELISA Assay Kit *Red Fluorescence* 1 kit
11541 Amplite™ Fluorimetric Goat Anti_Rabbit IgG_HRP Conjugate ELISA Assay Kit *Red Fluorescence* 1 kit
11541 Amplite™ Fluorimetric Goat Anti_Rabbit IgG_HRP Conjugate ELISA Assay Kit *Red Fluorescence* 1 kit
11540 Amplite Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence* 1 kit
A1113 A2460 A2330 K01 Amplite™ Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence* 1 kit
A1113 A2460 A2330 K01 Amplite™ Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence* 1 kit
MatrixAHGCrossmatchCard For Direct Coombs Test, Indirect Coombs test, Indirect Antibody test, Antibody Screening and identification. (6 test per card) 24 cards
27-629 Trans-acting T-cell specific transcription factor GATA-3 is a member of GATA family of transcription factors that regulates development of multiple tissues. It is an important transcription factor in 0.05 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur