Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :23986534
Publication Date : //

Differential response of regulatory and conventional CD4⁺ lymphocytes to CD3 engagement: clues to a possible mechanism of anti-CD3 action?


Several clinical trials have shown anti-CD3 treatment to be a promising therapy for autoimmune diabetes, but its mechanism of action remains unclear. Foxp3(+) regulatory T cells (Tregs) are likely to be involved, but through unknown mechanistic pathways. We profiled the transcriptional consequences in CD4(+) Tregs and conventional T cells (Tconvs) in the first hours and days after anti-CD3 treatment of NOD mice. Anti-CD3 treatment led to a transient transcriptional response, terminating faster than most Ag-induced responses. Most transcripts were similarly induced in Tregs and Tconvs, but several were differential, in particular, those encoding the IL-7R and transcription factors Id2/3 and Gfi1, upregulated in Tregs but repressed in Tconvs. Because IL-7R was a plausible candidate for driving the homeostatic response of Tregs to anti-CD3, we tested its relevance by supplementation of anti-CD3 treatment with IL-7/anti-IL-7 complexes. Although ineffective alone, IL-7 significantly improved the rate of remission induced by anti-CD3. Four anti-human CD3 mAbs exhibited the same differential effect on IL-7R expression in human as in mouse cells, suggesting that the mechanism also underlies therapeutic effect in human cells, and perhaps a rationale for testing a combination of anti-CD3 and IL-7 for the treatment of recent-onset human type 1 diabetes. Thus, systems-level analysis of the response to anti-CD3 in the early phase of the treatment demonstrates different responses in Tregs and Tconvs, and provides new leads to a mechanistic understanding of its mechanism of action in reverting recent-onset diabetes.

Authors : Li Li , Nishio Junko , van Maurik André , Mathis Diane , Benoist Christophe ,

Related products :

Catalog number Product name Quantity
orb61325 Docetaxel Docetaxel, a semisynthetic analog of paclitaxel, shares the latter's mechanism of action For research use only. 300 mg
orb82622 Rat IL21 protein IL21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological 2
orb84796 IL21 protein IL21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigenspecific responses of immune cells. The biological effec 10
27-567 SKIL belongs to the SKI family and may have regulatory role in cell division or differentiation in response to extracellular signals. 0.1 mg
27-665 IFN regulatory factor (IRF)-4 is a lymphoid_myeloid-restricted member of the IRF transcription factor family that plays an essential role in the homeostasis and function of mature lymphocytes. IRF-4 e 0.1 mg
28-806 IFN regulatory factor (IRF)-4 is a lymphoid_myeloid-restricted member of the IRF transcription factor family that plays an essential role in the homeostasis and function of mature lymphocytes. IRF-4 e 0.05 mg
EIAAB25653 Homo sapiens,hPCl2,Human,Metal regulatory transcription factor 2,Metal-response element DNA-binding protein M96,Metal-response element-binding transcription factor 2,MTF2,PCL2,Polycomb-like protein 2
EIAAB25652 Metal regulatory transcription factor 2,Metal-response element DNA-binding protein M96,Metal-response element-binding transcription factor 2,Mouse,mPCl2,Mtf2,Mus musculus,Pcl2,Polycomb-like protein 2,
18-003-42503 Metal-response element-binding transcription factor 2 - Metal-response element DNA-binding protein M96; Metal-regulatory transcription factor 2; PCL2 Polyclonal 0.05 mg Aff Pur
SCH-MCA751 MOUSE ANTI GUINEA PIG T LYMPHOCYTES, Product Type Monoclonal Antibody, Specificity T LYMPHOCYTES , Target Species Guinea Pig, Host Mouse, Format Ascites, Isotypes IgG1, Applications C, F, P, Cl 0.25 ml
SCH-MCA751APC MOUSE ANTI GUINEA PIG T LYMPHOCYTES APC, Product Type Monoclonal Antibody, Specificity T LYMPHOCYTES , Target Species Guinea Pig, Host Mouse, Format APC, Isotypes IgG1, Applications F, Clone C 100 TESTS
MCA564 MOUSE ANTI GUINEA PIG T LYMPHOCYTES, Product Type Monoclonal Antibody, Specificity T LYMPHOCYTES, Target Species Guinea Pig, Host Mouse, Format S_N, Isotypes IgM, Applications C, F, Clone MsGp 2 ml
MCA751APC MOUSE ANTI GUINEA PIG T LYMPHOCYTES APC, Product Type Monoclonal Antibody, Specificity T LYMPHOCYTES , Target Species Guinea Pig, Host Mouse, Format APC, Isotypes IgG1, Applications F, Clone C 100 TESTS
SCH-MCA564 MOUSE ANTI GUINEA PIG T LYMPHOCYTES, Product Type Monoclonal Antibody, Specificity T LYMPHOCYTES, Target Species Guinea Pig, Host Mouse, Format S_N, Isotypes IgM, Applications C, F, Clone MsGp 2 ml
MCA751 MOUSE ANTI GUINEA PIG T LYMPHOCYTES, Product Type Monoclonal Antibody, Specificity T LYMPHOCYTES , Target Species Guinea Pig, Host Mouse, Format Ascites, Isotypes IgG1, Applications C, F, P, Cl 0.25 ml
B216 L.S. Differential Medium Base (Lactobacillus Streptococcus Differential Medium Base) USE For differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, TTC reduction and Qty per Litre of Medium: 65,3
B1193 Lactobacillus Streptococcus Differential Medium Base (L.S. Differential Medium Base) USE For differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, TTC reduction and Qty per Litre of Medium: 65,3
B216 L.S. Differential Medium Base (Lactobacillus Streptococcus Differential Medium Base) USE For differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, TTC reduction and Qty per Litre of Medium: 65,3
B1193 Lactobacillus Streptococcus Differential Medium Base (L.S. Differential Medium Base) USE : For differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, TTC reduction and 5x500gm
B216 L.S. Differential Medium Base (Lactobacillus Streptococcus Differential Medium Base) USE : For differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, TTC reduction and 5x500gm
B1193 Lactobacillus Streptococcus Differential Medium Base (L.S. Differential Medium Base) USE For differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, TTC reduction and Qty per Litre of Medium: 65,3
SCH-MCA560 MOUSE ANTI GUINEA PIG LYMPHOCYTES AND LANGERHANS CELLS, Product Type Monoclonal Antibody, Specificity LYMPHOCYTES, Target Species Guinea Pig, Host Mouse, Format S_N, Isotypes IgG1, Applications 2 ml
MCA560 MOUSE ANTI GUINEA PIG LYMPHOCYTES AND LANGERHANS CELLS, Product Type Monoclonal Antibody, Specificity LYMPHOCYTES, Target Species Guinea Pig, Host Mouse, Format S_N, Isotypes IgG1, Applications 2 ml
SCH-MCA800G MOUSE ANTI RABBIT T LYMPHOCYTES, Product Type Monoclonal Antibody, Specificity T LYMPHOCYTES, Target Species Rabbit, Host Mouse, Format Purified, Isotypes IgG1, Applications C, F, IP, Clone KE 0.25 mg
SCH-MCA800 MOUSE ANTI RABBIT T LYMPHOCYTES, Product Type Monoclonal Antibody, Specificity T LYMPHOCYTES, Target Species Rabbit, Host Mouse, Format S_N, Isotypes IgG1, Applications C, F, IP, Clone KEN_5 2 ml


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur