Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :24066895
Publication Date : //

Chicken astrovirus capsid proteins produced by recombinant baculoviruses: potential use for diagnosis and vaccination.


Chicken astroviruses (CAstVs) have been characterized recently. Due to their relatively poor growth in cell culture, virus-specific antigens are not readily available for the development of diagnostic reagents and vaccines. For this purpose two capsid protein antigens, specified by the 11672 isolate of CAstV, were produced in insect cells following infection with recombinant baculoviruses. The GST-11672 capsid protein, a fusion protein comprising the capsid protein and glutathione-S-transferase (GST) as an N-terminal affinity tag, and the 11672 capsid protein alone were detected by western blotting as proteins of ~100 and 70 kDa, respectively. Immunization with the affinity-purified GST-11672 capsid protein produced a polyclonal rabbit antiserum, which reacted by indirect immunofluorescence with Group B CAstVs but which showed no reactivity with the Group A CAstV isolate, 612. When used as part of an immunoperoxidase-based immunohistochemical procedure, this rabbit antiserum facilitated the detection of CAstV antigen in formalin-fixed, paraffin-embedded kidney tissue at the sites of histopathology characteristic of nephritis. Although further evaluation with sera from commercial chickens is required, a prototype indirect antibody-detecting enzyme-linked immunosorbent assay (ELISA) based on affinity-purified GST-11672 capsid protein as coating antigen demonstrated considerable potential with low ELISA absorbance values being generated with sera from specific pathogen free (SPF) chickens, and high absorbance values being generated with serum samples from experimentally infected chickens. Immunization experiments of SPF chickens showed that, when administered as mixtures with oil adjuvant, crude cell lysates containing the GST-11672 capsid protein or the 11672 capsid protein elicited virus-specific antibody responses that were detectable by indirect immunofluorescence and by virus neutralization assays.

Authors : Lee A , Wylie M , Smyth V J , Skibinska A , Patterson I A , Forster F , Welsh M D , Todd D ,

Related products :

Catalog number Product name Quantity
ODZ-186 Astrovirus Rapid-VIDITEST for diagnosis of gastro-intestinal infection card 5 kits
ODZ-253 Astrovirus Rapid-VIDITEST for diagnosis of gastro-intestinal infection blister 5 kits
ODZ-186 Diagnosis of gastro-intestinal infection, Rapid-VIDITEST Astrovirus Card 20 tests
ODZ-253 Diagnosis of gastro-intestinal infection, Rapid-VIDITEST Astrovirus Blister 20 tests
ODZ-253 Rapid-VIDITEST: Astrovirus Blister Diagnosis of gastro-intestinal infection 20 Tests in kit
ODZ-186 Rapid-VIDITEST: Astrovirus Card Diagnosis of gastro-intestinal infection 20 Tests in kit
C01297M MAb to Astrovirus (Capsid) 1mg
MD-14-0742 Mouse Anti-Astrovirus (Capsid) 1 mg
AM00813PU-N Astrovirus Type 2 Capsid Protein 1 mg
AM00813PU-N Astrovirus Type 2 Capsid Protein 1 mg
C01297M Astrovirus Capsid ProteinMouse Purified IgG1 1mg
C01297M Astrovirus Capsid Protein host: Mouse 1mg
MD-14-0742 Mouse Anti-Astrovirus Capsid Protein Antibodies 1 mg
GWB-T00714 Anti- Astrovirus Capsid Protein Antibody - Clone B314M
'AM00813PU-N Astrovirus Type 2 (Capsid protein) IgG1 antibody Ab host: Mouse 1 mg
AM00813PU-N Astrovirus Type 2 (Capsid protein) Mouse IgG1 antibody Ab Purified 1 mg
A10S ProEasy. Vector for easy construction of recombinant baculoviruses. 25 ul
AM00813PU-N Astrovirus Type 2 (Capsid protein) Clone B314M antibody Isotype IgG1 Host Mouse 1 mg
A10S ProEasy. Vector for easy construction of recombinant baculoviruses, Host: Spodoptera frugiperda, Trichoplusia ni 25 ul
MD-05-0153P Recombinant Rubella Capsid Proteins 1 mg
228-11385-3 Recombinant Rubella Viral Capsid Proteins 1000
228-11385-2 Recombinant Rubella Viral Capsid Proteins 500
228-11385-1 Recombinant Rubella Viral Capsid Proteins 100
orb23374 Astrovirus (Capsid) antibody Monoclonal Antibodies Primary antibodies 1 mg
orb23374 Astrovirus (Capsid) antibody Monoclonal Antibody Host: Mouse 1mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur