Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :24497893
Publication Date : //

Unique Photobleaching Phenomena of the Twin-Arginine Translocase Respiratory Enzyme Chaperone DmsD.


DmsD is a chaperone of the redox enzyme maturation protein family specifically required for biogenesis of DMSO reductase in Escherichia coli. It exists in multiple folding forms, all of which are capable of binding its known substrate, the twin-arginine leader sequence of the DmsA catalytic subunit. It is important for maturation of the reductase and targeting to the cytoplasmic membrane for translocation. Here, we demonstrate that DmsD exhibits an irreversible photobleaching phenomenon upon 280 nm excitation irradiation. The phenomenon is due to quenching of the tryptophan residues in DmsD and is dependent on its folding and conformation. We also show that a tryptophan residue involved in DmsA signal peptide binding (W87) is important for photobleaching of DmsD. Mutation of W87, or binding of the DmsA twin-arginine signal peptide to DmsD in the pocket that includes W72, W80, and W91 significantly affects the degree of photobleaching. This study highlights the advantage of a photobleaching phenomenon to study protein folding and conformation changes within a protein that was once considered unusable in fluorescence spectroscopy.

Authors : Rivardo Fabrizio , Leach Thorin G H , Chan Catherine S , Winstone Tara M L , Ladner Carol L , Sarfo Kwabena J , Turner Raymond J ,

Related products :

Catalog number Product name Quantity
26-325 GATM is a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to 0.05 mg
26-283 OAT is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations of this enzyme cause the autosomal rece 0.05 mg
30-358 OAT is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations of this enzyme cause the autosomal rece 0.1 mg
26-342 ATE1 is an arginyltransferase, an enzyme that is involved in posttranslational conjugation of arginine to N-terminal aspartate or glutamate residues. Conjugation of arginine to the N-terminal aspartat 0.05 mg
29-801 Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxy 0.1 mg
26-196 A4GNT is a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Gal 0.05 mg
30-271 A4GNT is a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Gal 0.05 mg
30-272 A4GNT is a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Gal 0.05 mg
M2010 Opti-BrytTM Antifade Mountant, Fast setting antifade mountant for increased signal intensity and minimized photobleaching, 5mL
EIAAB05333 CARM1,Coactivator-associated arginine methyltransferase 1,Histone-arginine methyltransferase CARM1,Homo sapiens,Human,PRMT4,Protein arginine N-methyltransferase 4
EIAAB05332 Carm1,Coactivator-associated arginine methyltransferase 1,Histone-arginine methyltransferase CARM1,Mouse,Mus musculus,Prmt4,Protein arginine N-methyltransferase 4
EIAAB05331 Carm1,Coactivator-associated arginine methyltransferase 1,Histone-arginine methyltransferase CARM1,Prmt4,Protein arginine N-methyltransferase 4,Rat,Rattus norvegicus
DnaK-312E Protein Recombinant E.coli DnaK Chaperone Hsp70, Co-chaperone with DnaJ (385-546);BioresearchProd(Pea ) 20ug
DnaK-312E Protein: Recombinant E.coli DnaK Chaperone Hsp70, Co-chaperone with DnaJ (385-546);BioresearchProd(Pea ) 20ug
30-092 Arginase catalyzes the hydrolysis of arginine to ornithine and urea. The type I isoform of ARG1, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inheri 0.1 mg
30-093 Arginase catalyzes the hydrolysis of arginine to ornithine and urea. The type I isoform of ARG1, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inheri 0.1 mg
DnaJ-317E Protein: Recombinant E.coli DnaJ Chaperone Hsp40, Co-chaperone with DnaK (1-376) 20ug
DnaJ-317E Protein Recombinant E.coli DnaJ Chaperone Hsp40, Co-chaperone with DnaK (1-376) 20ug
HPAI-RR Human PAI_1 (substrate form _ P12 Arginine P14 Arginine double mutant) 1,0 mg
HPAI-RR Human PAI_1 (substrate form _ P12 Arginine P14 Arginine double mutant) 0,5 mg
HPAI-RR Human PAI-1 (substrate form - P12 Arginine P14 Arginine double mutant) 0.5 mg
HPAI-RR Human PAI-1 (substrate form – P12 Arginine P14 Arginine double mutant) 1.0 mg
HPAI-RR Human PAI-1 (substrate form - P12 Arginine P14 Arginine double mutant) 1.0 mg
HPAI-RR PAI - 1 Human PAI-1 (substrate form - P12 Arginine P14 Arginine double mutant) 1.0 mg
HPAI-RR Human PAI-1 (substrate form – P12 Arginine P14 Arginine double mutant) 0.5 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur