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Pubmed ID :25803499
Publication Date : //

Synergistic Effect of Neutral Protease and Clostripain on Rat Pancreatic Islet Isolation.


Islet isolation currently requires collagenase, neutral protease and other components. Thermolysin (TL) from Bacillus thermoproteolyticus is the gold standard neutral protease. However, we speculated that neutral protease derived from Clostridium histolyticum (Ch; ChNP) would be biologically superior for islet isolation. Tryptic-like activity has also been reported to be important. Therefore, we focused on clostripain (CP), since it is one of the main proteases in Clostridium histolyticum which possesses tryptic-like activity. We then examined the synergistic effects of highly purified ChNP and CP on rat islet isolation.

Authors : Dendo Mami , Maeda Hiroshi , Yamagata Youhei , Murayama Kazutaka , Watanabe Kimiko , Imura Takehiro , Inagaki Akiko , Igarashi Yasuhiro , Katoh Yasutake , Ebina Masayuki , Fujimori Keisei , Igarashi Kazuhiko , Ohuchi Noriaki , Satomi Susumu , Goto Masafumi ,

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