Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :26239052
Publication Date : //

Dendritic cells combined with anti-GITR antibody produce antitumor effects in osteosarcoma.


We attempted to enhance the antitumor effects of tumor lysate-pulsed dendritic cells by eliminating regulatory T cells. The combinatorial effects of dendritic cells and agonist anti-glucocorticoid-induced tumor necrosis factor receptor (anti-GITR) antibodies were investigated with respect to enhancement of the systemic immune response, elimination of regulatory T cells, and inhibition of tumor growth. To determine whether the combination of dendritic cells and anti‑GITR antibodies could enhance systemic immune responses and inhibit primary tumor growth in a murine osteosarcoma (LM8) model. We established the following 4 groups of C3H mice (20 mice in total): i), control IgG-treated mice; ii), tumor lysate-pulsed dendritic cell‑treated mice; iii), agonist anti-GITR antibody-treated mice; and iv), agonist anti-GITR antibody- and tumor lysate-pulsed dendritic cell‑treated mice.The mice that received the agonist anti-GITR antibodies and tumor lysate-pulsed dendritic cells displayed inhibited primary growth, prolonged life time, reduced numbers of regulatory T lymphocytes in the spleen, elevated serum interferon-γ levels, increased number of CD8+ T lymphocytes. The mice that received combined therapy had reduced level of immunosuppressive cytokines in tumor tissue and serum. Combining agonist anti-GITR antibodies with tumor lysate-pulsed dendritic cells enhanced the systemic immune response. These findings provide further support for the continued development of agonist anti-GITR antibodies as an immunotherapeutic strategy for osteosarcoma. We suggest that our proposed immunotherapy could be developed further to improve osteosarcoma treatment.

Authors : Kawano Masanori , Tanaka Kazuhiro , Itonaga Ichiro , Iwasaki Tatsuya , Miyazaki Masashi , Ikeda Shinichi , Tsumura Hiroshi ,

Related products :

Catalog number Product name Quantity
SCH-MCA1121 MOUSE ANTI HUMAN DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity DENDRITIC CELLS_B CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applications C, Clone 0.2 mg
MCA1121 MOUSE ANTI HUMAN DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity DENDRITIC CELLS_B CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applications C, Clone 0.2 mg
orb82779 Human TNF alpha Variant protein The clinical use of the potent antitumor activity of TNF-alpha has been limited by the proinflammatory side effects including fever, dose-limiting hypotension, hepatoto 1 mg
MCA2494 MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applicatio 0.2 mg
SCH-MCA2494T MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applicatio 25 µg
SCH-MCA2494 MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applicatio 0.2 mg
MCA2494T MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applicatio 25 µg
SCH-4562-0408 MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applicati 0.2 mg
4562-0408 MOUSE ANTI HUMAN FOLLICULAR DENDRITIC CELLS, Product Type Monoclonal Antibody, Specificity FOLLICULAR DENDRITIC CELLS, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applicati 0.2 mg
orb82810 Mouse MDC protein CCL22_MDC is a CC chemokine that is produced in B cells, macrophages, monocytederived dendritic cells, activated NK cells and CD4 T cells. It signals through the CCR4 receptor. MDC c 1 mg
orb82743 Human MDC protein MDC is a CC chemokine that is produced in B cells, macrophages, monocyte-derived dendritic cells, activated NK cells and CD4 T cells. It signals through the CCR4 receptor. MDC chemoa 1 mg
20-321-175230 DENDRITIC CELLS - MONOCLONAL ANTIBODY TO RAT DENDRITIC CELLS Monoclonal 0.1 mg
DS-MB-01157 Mouse Anti-Human Dendritic Cells, B cells Antibodies 0.2 mg
'SM064P Dendritic cells + Interdigitating cells IgG2a antibody Ab host: Rat 0.2 mg
'SM064 Dendritic cells + Interdigitating cells IgG2a antibody Ab host: Rat 2 ml
SM064P Dendritic cells + Interdigitating cells Rat IgG2a antibody Ab Purified 0.2 mg
SM064 Dendritic cells + Interdigitating cells Rat IgG2a antibody Ab Supernatant 2 ml
orb61300 Vorinostat Vorinostat is a histone deacetylase inhibitor and causes growth arrest and death of some transformed cells both in vitro and in vivo, with little or no toxic effects on normal cells. For re 1 g
SM1210 Dendritic cells + B-cells Mouse IgM antibody Ab Supernatant 0.2 mg
'SM1210 Dendritic cells + B-cells IgM antibody Ab host: Mouse 0.2 mg
26-760 DCAL1 is a type II transmembrane, C-type lectin-like protein expressed on dendritic cells (DCs) and B cells. It interacts with subsets of T cells as a costimulatory molecule that enhances interleukin- 0.05 mg
26-759 DCAL1 is a type II transmembrane, C-type lectin-like protein expressed on dendritic cells (DCs) and B cells. It interacts with subsets of T cells as a costimulatory molecule that enhances interleukin- 0.05 mg
20-272-191212 Follicular Dendritic Cells - Rat monoclonal [ER - MP20 ] to Follicular Dendritic Cells Monoclonal 0.05 mg
cd19 250 HUMAN CELL SURFACE MOLECULES cd B4419 dendritic cells, B cells 250 nm 2 ml
cd19 50 HUMAN CELL SURFACE MOLECULES cd B4419 dendritic cells, B cells 50 nm 2 ml


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur