Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :26517714
Publication Date : //

Identification of Host-Plant Volatiles and Characterization of Two Novel General Odorant-Binding Proteins from the Legume Pod Borer, Maruca vitrata Fabricius (Lepidoptera: Crambidae).


Chemoreception is a key feature in selection of host plant by phytophagous insects, and odorant-binding proteins (OBPs) are involved in chemical communication of both insects and vertebrates. The legume pod borer, Maruca vitrata Fabricius (Lepidoptera: Crambidae) is one of the key pest species of cowpea and widely distributed throughout tropical and subtropical regions, causing up to 80% of yield loss. In this study, we investigated the electrophysiological responses of female M. vitrata to floral volatiles from V. unguiculata. Seventeen electroantennogram-active compounds were identified from floral volatiles of V. unguiculata by coupled gas chromatography-electroantennography (GC-EAD) and gas chromatography-mass spectrometry (GC-MS). Then, we cloned two novel full-length GOBP genes (MvitGOBP1 and MvitGOBP2) from the antennae of M. vitrata using reverse transcription PCR. Protein sequence analysis indicated that they shared high sequence similarity with other Pyralididae insect GOBPs and had the typical six-cysteine signature. Real-time PCR analysis indicated that MvitGOBP1-2 mRNA was highly expressed in the antennae of female adult with several thousands-fold difference compare to other tissue. Next, the recombinant MvitGOBP1-2 was expressed in Escherichia coli and purified using Ni ion affinity chromatography. Fluorescence binding assays demonstrated that MvitGOBP1-2 had different binding affinities with 17 volatile odorant molecules including butanoic acid butyl ester, limonene, 4-ethylpropiophenone, 1H-indol-4-ol, butanoic acid octyl ester and 2-methyl-3-phenylpropanal. In the field trapping experiment, these six floral volatiles could effectively attract female moths and showed significant difference compared with the blank lure. These results suggested that MvitGOBPs and the seventeen floral volatiles are likely to function in the olfactory behavior response of female moths, which may have played crucial roles in the selection of oviposition sites. The six compounds that we have identified from the volatiles of V. unguiculata may provide useful information for exploring efficiency monitoring and integrated pest management strategies of this legume pod borer in the field.

Authors : Zhou Jing , Zhang Na , Wang Pan , Zhang Shichang , Li Daiqin , Liu Kaiyu , Wang Guoxiu , Wang Xiaoping , Ai Hui ,

Related products :

Catalog number Product name Quantity
25-780 GTP-binding proteins, or G proteins, constitute a superfamily capable of binding GTP or GDP. G proteins are activated by binding GTP and are inactivated by hydrolyzing GTP to GDP. This general mechani 0.05 mg
29-770 GTP-binding proteins, or G proteins, constitute a superfamily capable of binding GTP or GDP. G proteins are activated by binding GTP and are inactivated by hydrolyzing GTP to GDP. This general mechani 0.1 mg
EIAAB28593 Homo sapiens,Human,OBP2B,OBPIIb,Odorant-binding protein 2b,Odorant-binding protein IIb,UNQ653_PRO1283
EIAAB28592 Homo sapiens,Human,OBP2A,OBPIIa,Odorant-binding protein 2a,Odorant-binding protein IIa
27-179 OR10X1 is an odorant receptor.Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins 0.05 mg
XGC-BiRDS-ChipSeq12 Bioinformatics analysis of ChipSeq data for identification of binding sites for DNA-associated proteins Upto 12 samples
02-044 Taq SSB (Single-Stranded DNA Binding) protein Proteins, General Reagents 100 ug
02-042-5 E. coli SSB (Single-Stranded DNA Binding) protein Proteins, General Reagents 5 x 200 ug
02-042 E. coli SSB (Single-Stranded DNA Binding) protein Proteins, General Reagents 200 ug
02-044 Taq SSB (Single-Stranded DNA Binding) protein Proteins, General Reagents DNA Repair_Recombination 100 ug
02-042 E. coli SSB (Single-Stranded DNA Binding) protein Proteins, General Reagents DNA Repair_Recombination 200 ug
02-042-5 E. coli SSB (Single-Stranded DNA Binding) protein Proteins, General Reagents DNA Repair_Recombination 5 x 200 ug
SPC-312D Hsp70_Hsc70 (Plant) Host Species Rabbit Species Reactivity Antibodies will recognize Hsp70 proteins from the following samples A. thaliana, radish, barley, lettuce, fish, other mammalian and plant 100ul
SPC-312D Hsp70_Hsc70 (Plant) Host Species Rabbit Species Reactivity Antibodies will recognize Hsp70 proteins from the following samples A. thaliana, radish, barley, lettuce, fish, other mammalian and plant 100ul
SPC-312D Hsp70_Hsc70 (Plant) Host Species Rabbit Species Reactivity Antibodies will recognize Hsp70 proteins from the following samples A. thaliana, radish, barley, lettuce, fish, other mammalian and plant 100ul
SPC-312D Hsp70_Hsc70 (Plant) Host Species Rabbit Species Reactivity Antibodies will recognize Hsp70 proteins from the following samples A. thaliana, radish, barley, lettuce, fish, other mammalian and plant 100ul
OBP_RAT Rat ELISA Kit FOR Odorant-binding protein 96T
E9793h Human Odorant Binding Protein 2A ELISA Kit 96T
E12033h Human Odorant Binding Protein 2B ELISA Kit 96T
OBSCN OBP2A Gene odorant binding protein 2A
OBSL1 OBP2B Gene odorant binding protein 2B
CSB-EL016251HU Human Odorant-binding protein 2a(OBP2A) ELISA kit 96T
CSB-EL016252HU Human Odorant-binding protein 2b(OBP2B) ELISA kit 96T
EIAAB28591 OBP,Obp1f,Odorant-binding protein,Rat,Rattus norvegicus
CSB-EL016251HU Human Odorant-binding protein 2a(OBP2A) ELISA kit SpeciesHuman 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur