Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :27035848
Publication Date : //

Macrophage migration inhibitory factor promotes cardiac stem cell proliferation and endothelial differentiation through the activation of the PI3K/Akt/mTOR and AMPK pathways.


Macrophage migration inhibitory factor (MIF) has pleiotropic immune functions in a number of inflammatory diseases. Recent evidence from expression and functional studies has indicated that MIF is involved in various aspects of cardiovascular disease. In this study, we aimed to determine whether MIF supports in vitro c-kit+CD45- cardiac stem cell (CSC) survival, proliferation and differentiation into endothelial cells, as well as the possible mechanisms involved. We observed MIF receptor (CD74) expression in mouse CSCs (mCSCs) using PCR and immunofluorescence staining, and MIF secretion by mCSCs using PCR and ELISA in vitro. Increasing amounts of exogenous MIF did not affect CD74 expression, but promoted mCSC survival, proliferation and endothelial differentiation. By contrast, treatment with an MIF inhibitor (ISO-1) or siRNA targeting CD74 (CD74‑siRNA) suppressed the biological changes induced by MIF in the mCSCs. Increasing amounts of MIF increased the phosphorylation of Akt and mammalian target of rapamycin (mTOR), which are known to support cell survival, proliferation and differentiation. These effects of MIF on the mCSCs were abolished by LY294002 [a phosphoinositide 3-kinase (PI3K) inhibitor] and MK-2206 (an Akt inhibitor). Moreover, adenosine monophosphate-activated protein kinase (AMPK) phosphorylation increased following treatment with MIF. The AMPK inhibitor, compound C, partly blocked the pro-proliferative effects of MIF on the mCSCs. In conclusion, our results suggest that MIF promotes mCSC survival, proliferation and endothelial differentiation through the activation of the PI3K/Akt/mTOR and AMPK signaling pathways. Thus, MIF may prove to be a potential therapeutic factor in the treatment of heart failure and myocardial infarction by activating CSCs.

Authors : Cui Jinjin , Zhang Fengyun , Wang Yongshun , Liu Jingjin , Ming Xing , Hou Jingbo , Lv Bo , Fang Shaohong , Yu Bo ,

Related products :

Catalog number Product name Quantity
orb82808 Mouse LIF protein Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of oth 1 mg
EIAAB32003 C20orf149,Exocrine differentiation and proliferation factor,EXPDF,Homo sapiens,Human,Pancreatic progenitor cell differentiation and proliferation factor,PPDPF
EIAAB32005 Bos taurus,Bovine,EXDPF,Exocrine differentiation and proliferation factor,Pancreatic progenitor cell differentiation and proliferation factor,PPDPF
EIAAB32004 Exdpf,Exocrine differentiation and proliferation factor,Mouse,Mus musculus,Pancreatic progenitor cell differentiation and proliferation factor,Ppdpf
EIAAB32002 Exdpf,Exocrine differentiation and proliferation factor,Pancreatic progenitor cell differentiation and proliferation factor,Ppdpf,Rat,Rattus norvegicus
EIAAB30425 C8orf22,Exocrine differentiation and proliferation factor-like protein,Homo sapiens,Human,Pancreatic progenitor cell differentiation and proliferation factor-like protein
CH33 Macrophage migration inhibitory factor MIF 500
CH27 Macrophage migration inhibitory factor MIF lmg
CH33 Macrophage migration inhibitory factor MIF lmg
CH27 Macrophage migration inhibitory factor MIF 500
CK-E98613 Rat Macrophage Migration Inhibitory Factor,MIF ELISAkit
E90698Hu ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) 96T/Kit
YHB0702Ra Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit 96T
DL-MIF-Mu Mouse Macrophage Migration Inhibitory Factor (MIF) ELISA Kit 96T
E90698Mu ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) 96T/Kit
E12411313 Rat Macrophage Migration Inhibitory Factor (MIF) ELISA Kit 1
RM014 Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit 96T
DL-MIF-Hu Human Macrophage Migration Inhibitory Factor (MIF) ELISA Kit 96T
E0372Ra Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit 48T
SL0469Ra Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit 96T
E02M0209 Rat Macrophage MigRation Inhibitory Factor ELISA 96T/kit
CSB-E07293r Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit 96T
Z03159-10 Macrophage Migration Inhibitory Factor (MIF), Human 10,0ug
Z03160-10 Macrophage Migration Inhibitory Factor (MIF), Mouse 10,0ug
KN0381Ra Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit 96 WELLS


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur