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Pubmed ID :27179568
Publication Date : //

Hepatitis B plasmonic biosensor for the analysis of clinical serum samples.


A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.

Authors : Riedel Tomáš , Surman František , Hageneder Simone , Pop-Georgievski Ognen , Noehammer Christa , Hofner Manuela , Brynda Eduard , Rodriguez-Emmenegger Cesar , Dostálek Jakub ,

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