Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery
Publication Date : //
Electropermeabilization of cell membranes by micro- and nanosecond-duration stimuli has been studied extensively, whereas effects of picosecond electric pulses (psEP) remain essentially unexplored. We utilized whole-cell patch clamp and Di-8-ANEPPS voltage-sensitive dye measurements to characterize plasma membrane effects of 500 ps stimuli in rat hippocampal neurons (RHN), NG108, and CHO cells. Even a single 500-ps pulse at 190 kV/cm increased membrane conductance and depolarized cells. These effects were augmented by applying brief psEP bursts (5-125 pulses), whereas the rate of pulse delivery (8Hz - 1 kHz) played little role. psEP-treated cells displayed large inward current at negative membrane potentials but modest or no conductance changes at positive potentials. A 1-kHz burst of 25 pulses increased the whole-cell conductance in the range (-100) - (-60) mV to 22-26 nS in RHN and NG108 cells (from 3 and 0.7 nS, respectively), but only to 5 nS in CHO (from 0.3 nS). The conductance increase was reversible within about 2 min. Such pattern of cell permeabilization, with characteristic inward rectification and slow recovery, was similar to earlier reported effects of 60- and 600-ns pulses, pointing to the similarity of structural membrane rearrangements in spite of a different membrane charging mechanism.
Authors : Semenov Iurii , Xiao Shu , Pakhomov Andrei G ,
Related products :
|Catalog number||Product name||Quantity|
|40-102||Electroporation Cuvette 4mm|
|40-100||Electroporation Cuvette 1mm|
|40-101||Electroporation Cuvette 2mm|
|BG011||15% Glycerol Electroporation Buffer w per 272 mM Sucrose||1000ml|
|40-100||Electroporation Cuvette 1mm Sterile, Individ. Wrapped||50 Cuvettes/Unit|
|40-102||Electroporation Cuvette 4mm Sterile, Individ. Wrapped||50 Cuvettes/Unit|
|40-101||Electroporation Cuvette 2mm Sterile, Individ. Wrapped||50 Cuvettes/Unit|
|BG011||15 percent Glycerol Electroporation Buffer w_ 272 mM Sucrose||5x1000ml|
|BE012||Electroporation buffer (E buffer)||1000ml|
52062 Aachen Deutschland
Support Karolina Elandt
Fax: (+49) 241 56 00 47 88
Logistic :0241 40 08 90 86
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
email@example.com | Gentaur
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123
Canada Montreal +15149077481
Ceská republika Praha +420246019719
Finland Helsset +358942419041
Magyarország Budapest +3619980547
US New York+17185132983
No related Items
Related Genes :
 Electroporation by subnanosecond pulses.
 Picosecond and Terahertz Perturbation of Interfacial Water and Electropermeabilization of Biological Membranes.
 Case for applying subnanosecond high-intensity, electrical pulses to biological cells.
 Subnanosecond electric pulses cause membrane permeabilization and cell death.
 Bioelectric effects of intense ultrashort pulses.
 Modified Blumlein pulse-forming networks for bioelectrical applications.
 Analysis of intense, subnanosecond electrical pulse-induced transmembrane voltage in spheroidal cells with arbitrary orientation.
 Plasma membrane voltage changes during nanosecond pulsed electric field exposure.
Enter catalog number :