GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Mass spectrometry-based proteomics revealed Glypican-1 as a novel ADAM17 substrate.

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :27576135
Publication Date : //

Mass spectrometry-based proteomics revealed Glypican-1 as a novel ADAM17 substrate.

ADAM17 (a disintegrin and metalloproteinase 17) is a plasma membrane metalloprotease involved in proteolytic release of the extracellular domain of many cell surface molecules, a process known as ectodomain shedding. Through this process, ADAM17 is implicated in several aspects of tumor growth and metastasis in a broad range of tumors, including head and neck squamous cell carcinomas (HNSCC). In this study, mass spectrometry-based proteomics approaches revealed glypican-1 (GPC1) as a new substrate for ADAM17, and its shedding was confirmed to be metalloprotease-dependent, induced by a pleiotropic agent (PMA) and physiologic ligand (EGF), and inhibited by marimastat. In addition, immunoblotting analysis of GPC1 in the extracellular media from control and ADAM17shRNA pointed to a direct involvement of ADAM17 in the cleavage of GPC1. Moreover, mass spectrometry-based interactome analysis of GPC1 revealed biological functions and pathways related mainly to cellular movement, adhesion and proliferation, which were events also modulated by up regulation of full length and cleavage GPC1. Altogether, we showed that GPC1 is a novel ADAM17 substrate, thus the function of GPC1 may be modulated by proteolysis signaling.

Authors : Kawahara Rebeca , Granato Daniela Campos , Yokoo Sami , Domingues Romênia Ramos , Trindade Daniel Maragno , Paes Leme Adriana Franco ,

Related products :

Catalog number	Product name	Quantity

S-0030.1	Protein Sequencing by Mass Spectrometry - Complex
S-0030	Protein Sequencing by Mass Spectrometry - Standard
S-0032	Post Translational Modification Mapping (PTM) by Mass Spectrometry
S-0030.2	Quantitative Mass Spectrometry using the Label free Quantitation protocol.
LI-011	PI3K Lipid Substrate Mix 1 PI, PE, PS, PC, and SM based on PI	1mg
LI-012	PI3K Lipid Substrate Mix 2 PI-4,5-P2, PE, PS, PC, and SM based on PI-4,5-P2	100
LI-011	PI3K Lipid Substrate Mix 1 PI, PE, PS, PC, and SM based on PI	1 mg
60B450	Polyclonal Antibodies: ADAM17; Specificity: ADAM17 ; Application: IHC	0.1mg
Adam17-8788R	Recombinant Rat Adam17, His-tagged species: Rat	20
DL-ADAM17-Hu	Human A Disintegrin And Metalloprotease 17 (ADAM17) ELISA Kit	96T
DL-ADAM17-Ra	Rat A Disintegrin And Metalloprotease 17 (ADAM17) ELISA Kit	96T
29-574	Mammalian sodium-dicarboxylate cotransporters transport succinate and other Krebs cycle intermediates. They fall into 2 categories based on their substrate affinity low affinity and high affinity. Bo	0.1 mg
29-575	Mammalian sodium-dicarboxylate cotransporters transport succinate and other Krebs cycle intermediates. They fall into 2 categories based on their substrate affinity low affinity and high affinity. Bo	0.1 mg
orb81300	Human Ras-Related C3 Botulinum Toxin Substrate 2 protein Recombinant RAC2 produced in E.coli is a single, non-glycosylated polypeptide chain containing 419 amino acids and having a molecular mass of 4	5
LF-MA41954	Anti-ADAM17 (1F6), Mouse Monoclonal to ADAM17, Isotype IgG2b, Host Mouse	50
LF-PA40018	anti-ADAM17, Rabbit polyclonal to ADAM17, Isotype IgG, Host Rabbit	50 ug
LF-PA42948	Anti-ADAM17, Goat Polyclonal to ADAM17, Isotype , Host Goat	50
LI-012	PI3K Lipid Substrate Mix 2 PI_4,5_P2, PE, PS, PC, and SM based on PI_4,5_P2	100µg
LI-012	PI3K Lipid Substrate Mix 2 PI_4,5_P2, PE, PS, PC, and SM based on PI_4,5_P2	100 µg
bs-2159P	Peptides: Glypican 4(Glypican proteoglycan 4) Protein Length:12-25 amino acids.	200ug lyophilized
UB-E02323	Human Laboratory testing revealed lymphocytic choriomeningitis virus antibody(LCMV-Ab)ELISA Kit	96T
E2041Hu	Human Laboratory testing revealed lymphocytic choriomeningitis virus antibody (LCMV-Ab)ELISA Kit	48T
E2041Hu	Human Laboratory testing revealed lymphocytic choriomeningitis virus antibody (LCMV-Ab)ELISA Kit	96T
201-12-2042	Human Laboratory testing revealed lymphocytic choriomeningitis virus antibody (LCMV-Ab)ELISA Kit	96T
201-12-2042	Human Laboratory testing revealed lymphocytic choriomeningitis virus antibody (LCMV-Ab)ELISA Kit	48T

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel: 0208-080893 Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62 SWIFT RABONL2U

GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur

ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF