Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :27622921
Publication Date : 2016/09/13

Biological reference materials for extracellular vesicle studies.


Extracellular vesicles (EVs) mediate normal physiological homeostasis and pathological processes by facilitating intercellular communication. Research of EVs in basic science and clinical settings requires both methodological standardization and development of reference materials (RM). Here, we show insights and results of biological RM development for EV studies. We used a three-step approach to find and develop a biological RM. First, a literature search was done to find candidates for biological RMs. Second, a questionnaire was sent to EV researchers querying the preferences for RM and their use. Third, a biological RM was selected, developed, characterized, and evaluated. The responses to the survey demonstrated a clear and recognized need for RM optimized for the calibration of EV measurements. Based on the literature, naturally occurring and produced biological RM, such as virus particles and liposomes, were proposed as RM. However, none of these candidate RMs have properties completely matching those of EVs, such as size and refractive index distribution. Therefore, we evaluated the use of nanoerythrosomes (NanoE), vesicles produced from erythrocytes, as a potential biological RM. The strength of NanoE is their resemblance to EVs. Compared to the erythrocyte-derived EVs (eryEVs), NanoE have similar morphology, a similar refractive index (1.37), larger diameter (70% of the NanoE are over 200nm), and increased positive staining for CD235a and lipids (Di-8-ANEPPS) (58% and 67% in NanoE vs. 21% and 45% in eryEVs, respectively). Altogether, our results highlight the general need to develop and validate new RM with similar physical and biochemical properties as EVs to standardize EV measurements between instruments and laboratories.

Authors : Valkonen S , van der Pol E , Böing A , Yuana Y , Yliperttula M , Nieuwland R , Laitinen S , Siljander P R M ,

Related products :

Catalog number Product name Quantity
B402 Anaerobic Agar w_o Dextrose USE For carbohydrate fermentation studies and for studies of haemolytic activity of Clostridia, Streptococci and other organisms. Qty per Litre of Medium: 38
B402 Anaerobic Agar w_o Dextrose For carbohydrate fermentation studies and for studies of haemolytic activity of Clostridia, Streptococci and other organisms 5x500gm
orb41362 VPS16 antibody Polyclonal Rabbit polyclonal to VPS16. Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in ye 100
orb41359 VPS11 antibody Polyclonal Rabbit polyclonal to VPS11. Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in ye 100
EIAAB40625 Rat,Rattus norvegicus,Sv2,Sv2a,Synaptic vesicle glycoprotein 2A,Synaptic vesicle protein 2,Synaptic vesicle protein 2A
EIAAB40624 Calcium regulator SV2A,Kiaa0736,Mouse,Mus musculus,Sv2,Sv2a,Synaptic vesicle glycoprotein 2A,Synaptic vesicle protein 2,Synaptic vesicle protein 2A
CEV20301 Human vWF ELISA Kit High (Positive) Reference Control (calibrated against WHO reference plasma)
CEF10071 Human Factor VII ELISA Kit High (Positive) Reference Control (calibrated against WHO reference standard)
CEF10111 Human Factor XI ELISA Kit High (Positive) Reference Control (calibrated against WHO reference standard)
CEF10101 Human Factor X ELISA Kit High (Positive) Reference Control (calibrated against WHO reference standard)
CEF10091 Human Factor IX ELISA Kit High (Positive) Reference Control (calibrated against WHO reference standard)
CET10011 Human tPA ELISA Kit High (Positive) Reference Plasma Control (calibrated against WHO reference standard)
CEF10051 Human Factor V ELISA Kit High (Positive) Reference Plasma Control (calibrated against WHO reference standard)
CEA33011 Human Antithrombin III ELISA Kit High (Positive) Reference Plasma Control (calibrated against WHO reference standard)
CEF20071 Human Factor VII ELISA Kit High (Positive) Reference Plasma Control (calibrated against WHO reference standard)
CEA33031 Human Antithrombin III ELISA Kit High (Positive) Reference Plasma Control (calibrated against WHO reference standard)
CEC10011 Human C-Reactive Protein ELISA Kit High (Positive) Reference Control (calibrated against WHO reference standard)
CEF20031 Human Ferritin ELISA Kit High (Positive) Reference Plasma Control (calibrated against WHO reference standard)
CEF10121 Human Factor XII ELISA Kit High (Positive) Reference Plasma Control (calibrated against WHO reference standard)
CEA80011 Human Serum Amyloid A ELISA Kit High (Positive) Reference Plasma Control (calibrated against WHO reference standard)
CEF10131 Human Factor XIII ELISA Kit High (Positive) Reference Plasma Control (calibrated against WHO reference standard)
2004 GenoExplorer™ miRNA qPCR Reference Primer Sets (selection of human-U6, human-U44, human-5SrRNA, mouse-U6, and mouse-5SrRNA)-Reference primer as an internal control reference (3µM each). Suggest to com 100
EIAAB37393 ER-Golgi SNARE of 24 kDa,ERS24,ERS-24,Homo sapiens,Human,SEC22 vesicle-trafficking protein homolog B,SEC22 vesicle-trafficking protein-like 1,SEC22B,SEC22L1,Vesicle-trafficking protein SEC22b
EIAAB37395 ER-Golgi SNARE of 24 kDa,ERS24,ERS-24,Mouse,mSec22b,Mus musculus,SEC22 vesicle-trafficking protein homolog B,SEC22 vesicle-trafficking protein-like 1,Sec22b,Sec22l1,Vesicle-trafficking protein SEC22b
EIAAB40659 Mouse,Mus musculus,Seminal vesicle protein 6,Seminal vesicle secretory protein 6,Seminal vesicle secretory protein VI,Svp6,SVS VI,Svs6,SVSP99


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur