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Enzymatic Synthesis of Glucose-Based Fatty Acid Esters in Bisolvent Systems Containing Ionic Liquids or Deep Eutectic Solvents.
Sugar fatty acid esters (SFAEs) are biocompatible nonionic surfactants with broad applications in food, cosmetic, and pharmaceutical industries. They can be synthesized enzymatically with many advantages over their chemical synthesis. In this study, SFAE synthesis was investigated by using two reactions: (1) transesterification of glucose with fatty acid vinyl esters and (2) esterification of methyl glucoside with fatty acids, catalyzed by Lipozyme TLIM and Novozym 435 respectively. Fourteen ionic liquids (ILs) and 14 deep eutectic solvents (DESs) were screened as solvents, and the bisolvent system composed of 1-hexyl-3-methylimidazolium trifluoromethylsulfonate ([HMIm][TfO]) and 2-methyl-2-butanol (2M2B) was the best for both reactions, yielding optimal productivities (769.6 and 397.5 µmol/h/g, respectively) which are superior to those reported in the literature. Impacts of different reaction conditions were studied for both reactions. Response surface methodology (RSM) was employed to optimize the transesterification reaction. Results also demonstrated that as co-substrate, methyl glucoside yielded higher conversions than glucose, and that conversions increased with an increase in the chain length of the fatty acid moieties. DESs were poor solvents for the above reactions presumably due to their high viscosity and high polarity.
Authors : Zhao Kai-Hua , Cai Yu-Zheng , Lin Xiao-Sheng , Xiong Jun , Halling Peter J , Yang Zhen ,
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WP2316: PPAR signaling pathway
WP1020: Fatty Acid Biosynthesis
WP105: Fatty Acid Beta Oxidation 2
WP1061: Fatty Acid Beta Oxidation
WP1107: Mitochondrial LC-Fatty Acid Beta-Oxidation
WP1139: Fatty Acid Biosynthesis
WP1177: Fatty Acid Beta Oxidation
WP1207: Fatty Acid Beta Oxidation
WP1226: Mitochondrial LC-Fatty Acid Beta-Oxidation
WP1228: Fatty Acid Biosynthesis
WP1237: Fatty Acid Beta Oxidation
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WP1269: Fatty Acid Beta Oxidation
WP1307: Fatty Acid Beta Oxidation
WP133: Fatty Acid Omega Oxidation
WP1352: Fatty Acid Biosynthesis
WP137: Fatty Acid Biosynthesis, Initial Steps
WP143: Fatty Acid Beta Oxidation
WP148: Fatty Acid Beta Oxidation 2
WP16: Fatty Acid Elongation, Unsaturated
WP1647: Fatty acid biosynthesis
WP1648: Fatty acid metabolism
WP169: Fatty Acid Beta Oxidation 3
WP1817: Fatty acid, triacylglycerol, and ketone body metabolism
WP187: Fatty Acid Beta Oxidation 2
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