Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28163253
Publication Date : 2017/02/06

Apoptotic death in erythrocytes of lamprey Lampetra fluviatilis induced by ionomycin and tert-butyl hydroperoxide.


The work examined the effects of Ca(2+) overload and oxidative damage on erythrocytes of river lamprey Lampetra fluvialtilis. The cells were incubated for 3h with 0.1-5μM Ca(2+) ionophore ionomycin in combination with 2.5mM Ca(2+) and 10-100μM pro-oxidant agent tert-butyl hydroperoxide (tBHP). The sensitivity of lamprey RBCs to studied compounds was evaluated by the kinetics of their death. Both toxicants induced dose- and time dependent phosphatidylserine (PS) externalization (annexin V-FITC labeling) and loss of membrane integrity (propidium iodide uptake). Highest doses of ionomycin (1-2μM) increased the number of PS-exposed erythrocytes to 7-9% within 3h, while 100μM tBHP produced up to 50% of annexin V-FITC-positive cells. Caspase inhibitor Boc-D-FMK (50μM), calpain inhibitor PD150606 (10μM) and broad protease inhibitor leupeptin (200μM) did not prevent ionomycin-induced PS externalization, whereas tBHP-triggered apoptosis was blunted by Boc-D-FMK. tBHP-dependent death of lamprey erythrocytes was accompanied by the decrease in relative cell size, loss of cell viability, activation of caspases 9 and 3/7, and loss of mitochondrial membrane potential, but all these processes were partially attenuated by Boc-D-FMK. None of examined death-associated events were observed in ionomycin-treated erythrocytes except activation of caspase-9. Incubation with ionomycin did not alter intracellular K(+) and Na(+) content, while exposure to tBHP resulted in 80% loss of K(+) and 2.8-fold accumulation of Na(+). Thus, lamprey erythrocytes appear to be more susceptible to oxidative damage. Ca(2+) overload does not activate the cytosolic death pathways in these cells.

Authors : Agalakova Natalia I , Ivanova Tatiana I , Gusev Gennadii P , Nazarenkova Anna V , Sufiyeva Dina A ,

Related products :

Catalog number Product name Quantity
B29190 tert.-Butyl hydroperoxide 70 percent 100 ML
B29190 tert.-Butyl hydroperoxide 70 percent 500 ML
B29190 tert-Butyl hydroperoxide 70 percent 500 ML
B29190 tert.-Butyl hydroperoxide 70 percent 1000 ML
B29190 tert-Butyl hydroperoxide 70 percent 1000 ML
B29190 tert-Butyl hydroperoxide 70 percent 100 ML
B691805 [(1S)-2-[[(tert-Butyl)dimethylsilyl]oxy]-1-(hydroxymethyl)ethyl]-carbamic Acid tert-Butyl Ester C14H31NO4Si CAS: 185692-85-7 500 mg
B-01920 BUTYLATED HYDROXY TOLUENE 99 percent (B.H.T) (2,6-di-Tert-Butyl-p-Cresol) (2,6-Di-Tert-Butyl-4-Methyl Phenol) CAS: 128-37-0 500 gm
B-01920 BUTYLATED HYDROXY TOLUENE 99 percent (B.H.T) (2,6-di-Tert-Butyl-p-Cresol) (2,6-Di-Tert-Butyl-4-Methyl Phenol) CAS: 128-37-0 10 x 500 gm
16990-60-6 O-tert-butyl-N-[(2-nitrophenyl)thio]-L-seri O-tert-butyl-N-[(2-nitrop 1g
220298-96-4 tert-Butyl n-[4-(aminomethyl)benzyl]carb tert-butyl n-[4-(aminom 1g
175135-87-2 tert-Butyl-2-[(4-chlorophenyl)thio]-2-meth tert-Butyl-2-[(4-chloroph 1g
174855-53-9 Tert-butyl 4-[2-(aminomethyl)phenyl]pipe Tert-butyl 4-[2-(aminom 1g
671212-34-3 tert-butyl (4-benzylpiperazin-1-yl)carbony tert-butyl (4-benzylpiper 1g
57360-63-1 N-[4-[4-(tert-butyl)phenoxy]phenyl]-1-hyd N-[4-[4-(tert-butyl)phen 1g
186650-98-6 Tert-butyl 4-(4-cyanophenyl)tetrahydro-1( Tert-butyl 4-(4-cyanoph 1g
95715-87-0 tert-Butyl (R)-(+)-4-formyl-2,2-dimethyl-3- tert-Butyl (R)-(+)-4-form 1g
844891-10-7 Tert-butyl 4-(4-bromobenzyl)piperazine-1 Tert-butyl 4-(4-bromob 1g
344566-78-5 Tert-butyl N-[1-(4-cyanophenyl)-4-piperid Tert-butyl N-[1-(4-cyan 1g
306935-44-4 tert-butyl N-[(4-cyclopropyl-5-mercapto-4 tert-butyl N-[(4-cyclopro 1g
294659-72-6 TERT-BUTYL(6-CHLORO-3-FORMYLP TERT-BUTYL(6-CHLO 1g
156866-52-3 Tert-butyl N-(4-formylbenzyl)carbamate Tert-butyl N-(4-formylb 1g
135632-53-0 Tert-butyl N-(4-piperidinylmethyl)carbam Tert-butyl N-(4-piperidin 1g
214834-18-1 tert-Butyl 4-(aminocarbothioyl)tetrahydro Tert-butyl 4-(aminocarb 1g
85153-88-4 tert-butyl 1,1,3,3-tetramethylbutyl peroxid tert-butyl 1,1,3,3-tetram 1g


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur