Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28185046
Publication Date : //

Complete Amino Acid Sequence of a Copper/Zinc-Superoxide Dismutase from Ginger Rhizome.


Superoxide dismutase (SOD) is an antioxidant enzyme protecting cells from oxidative stress. Ginger (Zingiber officinale) is known for its antioxidant properties, however, there are no data on SODs from ginger rhizomes. In this study, we purified SOD from the rhizome of Z. officinale (Zo-SOD) and determined its complete amino acid sequence using N terminal sequencing, amino acid analysis, and de novo sequencing by tandem mass spectrometry. Zo-SOD consists of 151 amino acids with two signature Cu/Zn-SOD motifs and has high similarity to other plant Cu/Zn-SODs. Multiple sequence alignment showed that Cu/Zn-binding residues and cysteines forming a disulfide bond, which are highly conserved in Cu/Zn-SODs, are also present in Zo-SOD. Phylogenetic analysis revealed that plant Cu/Zn-SODs clustered into distinct chloroplastic, cytoplasmic, and intermediate groups. Among them, only chloroplastic enzymes carried amino acid substitutions in the region functionally important for enzymatic activity, suggesting that chloroplastic SODs may have a function distinct from those of SODs localized in other subcellular compartments. The nucleotide sequence of the Zo-SOD coding region was obtained by reverse-translation, and the gene was synthesized, cloned, and expressed. The recombinant Zo-SOD demonstrated pH stability in the range of 5-10, which is similar to other reported Cu/Zn-SODs, and thermal stability in the range of 10-60 °C, which is higher than that for most plant Cu/Zn-SODs but lower compared to the enzyme from a Z. officinale relative Curcuma aromatica.

Authors : Nishiyama Yuki , Fukamizo Tamo , Yoneda Kazunari , Araki Tomohiro ,

Related products :

Catalog number Product name Quantity
EIAAB06217 Ccs,Ccsd,Copper chaperone for superoxide dismutase,Mouse,Mus musculus,Superoxide dismutase copper chaperone
EIAAB06215 CCS,Copper chaperone for superoxide dismutase,Homo sapiens,Human,Superoxide dismutase copper chaperone
NRPC01A Recombinant Human Copper, Zinc-Superoxide Dismutase rhCu,Zn-SOD1 500mg
NRPC01A Recombinant Human Copper, Zinc-Superoxide Dismutase rhCu,Zn-SOD1 100mg
NRPC01A Recombinant Human Copper, Zinc-Superoxide Dismutase rhCu,Zn-SOD1 10mg
NRPC01A Recombinant Human Copper, Zinc-Superoxide Dismutase rhCu,Zn-SOD1 1mg x 20
EIAAB06218 Ccs,Copper chaperone for superoxide dismutase,Rat,Rattus norvegicus,Superoxide dismutase copper chaperone
EIAAB06216 CCS,Copper chaperone for superoxide dismutase,Pig,Superoxide dismutase copper chaperone,Sus scrofa
orb81377 Human Superoxide Dismutase-1 protein Recombinant Human Cu_Zn Superoxide Dismutase produced in E.coli is a single monomeric non-glycosylated polypeptide chain containing 154 amino acids and having tota 5
orb80178 Human Superoxide Dismutase (homodimer) protein Recombinant Human Cu_Zn Superoxide Dismutase produced in E.coli is homodimer, non-glycosylated polypeptide chain containing 2x 154 amino acids and having 100
CCT2 CCS Gene copper chaperone for superoxide dismutase
pro-251 Recombinant Human Copper Chaperone for Superoxide Dismutase CCS 25
CCS_PIG Pig ELISA Kit FOR Copper chaperone for superoxide dismutase 96T
E02S0145 Rat Superoxide Dismutase, Copper Chaperone ELISA 96T/kit
pro-251 Recombinant Human Copper Chaperone for Superoxide Dismutase CCS 1mg
pro-251 Recombinant Human Copper Chaperone for Superoxide Dismutase 5
pro-251 Recombinant Human Copper Chaperone for Superoxide Dismutase 25
CSB-EL004854RA Rat Copper chaperone for superoxide dismutase(CCS) ELISA kit 96T
pro-251 Recombinant Human Copper Chaperone for Superoxide Dismutase CCS 5
RPR-251 Recombinant Human Copper Chaperone for Superoxide Dismutase 5
pro-251 Recombinant Human Copper Chaperone for Superoxide Dismutase 1mg
ER1348 Superoxide Dismutase 4, Copper Chaperone Elisa Kit 96T
E8880h Human Superoxide Dismutase, Copper Chaperone ELISA 96T
E07S0145 Porcine Superoxide Dismutase, Copper Chaperone ELISA 96T/kit
E04S0145 Rabbit Superoxide Dismutase, Copper Chaperone ELISA 96T/kit


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur